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公开(公告)号：US20070269854A1
公开(公告)日：2007-11-22
申请号：US11573797
申请日：2005-08-05
摘要： The invention concerns a method and a device for measuring an enzymatic activity in a body fluid. The method comprises the following steps: a) providing a support having a conductive surface whereon is immobilized a substrate specific of the enzymatic activity to be measured, the substrate comprising an electroactive residue; b) reacting a sample of the body fluid with the substrate so as to bring about an enzymatic reaction generating an electroactive product; c) detecting the amount of electroactive product by amperometric measurement using a support in contact with the medium containing the enzyme. The invention is useful in particular for measuring enzymatic activities related to blood coagulation.
摘要翻译： 本发明涉及用于测量体液中的酶活性的方法和装置。 该方法包括以下步骤：a）提供具有导电表面的载体，其固定有待测量的酶活性特异性的底物，所述底物包含电活性残基; b）使体液样品与底物反应，以产生产生电活性产物的酶反应; c）使用与含有该酶的培养基接触的载体，通过电流计测量检测电活性产物的量。 本发明特别用于测量与凝血有关的酶活性。
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2.发明授权Process for the production of a deposit of inorganic nanoparticles having micro gaps onto a light-transparent support 有权用于将具有微隙的无机纳米颗粒的沉积物生产到透光载体上的方法公开(公告)号：US08945670B2
公开(公告)日：2015-02-03
申请号：US13806478
申请日：2011-07-18
CPC分类号： B05D1/007 , C03C17/001 , C03C17/006 , C03C2217/425 , G02B1/11 , Y10T428/24355
摘要： The invention relates to a process for the production of a transparent support covered with a deposit of inorganic nanoparticles, said deposit having micro gaps, comprising the following steps: application of a solution of polyelectrolyte having ionized functional groups to a transparent support, followed by at least one washing and drying step to form a charged polyelectrolyte deposit on said support; application of a solution of polymer microparticles having ionized groups with charges opposed to those of the polyelectrolyte deposit to said polyelectrolyte deposit, followed by at least one washing step for the formation of a deposit of charged polymer microparticles on the polyelectrolyte deposit; covering the deposit of charged polymer microparticles with a solution of polyelectrolyte having ionized functional groups with charges opposed to those of the charged polymer microparticles of step (b), followed by at least one washing and drying step to form a charged polyelectrolyte deposit; application of a solution of ionized inorganic nanoparticles having surface charges opposed to those of the charges of the polyelectrolyte of step (c) to the charged polyelectrolyte deposit, followed by at least one washing step to form at least one layer of ionized inorganic nanoparticles; removal of the deposit of polymer microparticles of step (b) and the deposits of polyelectrolyte of steps (a) and (c) to obtain the deposit of inorganic nanoparticles having micro gaps.
摘要翻译： 本发明涉及一种制备覆盖有无机纳米颗粒沉积物的透明载体的方法，所述沉积物具有微隙，包括以下步骤：将具有离子化官能团的聚电解质溶液施加到透明载体上，随后在 至少一个洗涤和干燥步骤以在所述载体上形成带电的聚电解质沉积物; 将具有与聚电解质沉积物相反的电荷的电离基团的聚合物微粒溶液施加到所述聚电解质沉积物上，随后至少一个洗涤步骤用于在聚电解质沉积物上形成带电聚合物微粒的沉积物; 用具有离子化官能团的聚电解质溶液覆盖带电聚合物微粒的沉积物，电荷与步骤（b）的带电聚合物微粒的电荷相反，然后进行至少一个洗涤和干燥步骤以形成带电的聚电解质沉积物; 将具有与步骤（c）的聚电解质的电荷相反的表面电荷的电离无机纳米颗粒溶液施加到带电的聚电解质沉积物中，随后进行至少一个洗涤步骤以形成至少一层电离的无机纳米颗粒; 去除步骤（b）的聚合物微粒的沉积物和步骤（a）和（c）的聚电解质的沉积物，以获得具有微隙的无机纳米颗粒的沉积。
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3.发明申请PROCESS FOR THE PRODUCTION OF A DEPOSIT OF INORGANIC NANOPARTICLES HAVING MICRO GAPS ONTO A LIGHT-TRANSPARENT SUPPORT 有权生产具有微小透镜的无机纳米颗粒沉积在光透明支持上的方法公开(公告)号：US20130108832A1
公开(公告)日：2013-05-02
申请号：US13806478
申请日：2011-07-18
IPC分类号： B05D1/00
CPC分类号： B05D1/007 , C03C17/001 , C03C17/006 , C03C2217/425 , G02B1/11 , Y10T428/24355
摘要： The invention relates to a process for the production of a transparent support covered with a deposit of inorganic nanoparticles, said deposit having micro gaps, comprising the following steps: application of a solution of polyelectrolyte having ionised functional groups to a transparent support, followed by at least one washing and drying step to form a charged polyelectrolyte deposit on said support; application of a solution of polymer microparticles having ionised groups with charges opposed to those of the polyelectrolyte deposit to said polyelectrolyte deposit, followed by at least one washing step for the formation of a deposit of charged polymer microparticles on the polyelectrolyte deposit; covering the deposit of charged polymer microparticles with a solution of polyelectrolyte having ionised functional groups with charges opposed to those of the charged polymer microparticles of step (b), followed by at least one washing and drying step to form a charged polyelectrolyte deposit; application of a solution of ionised inorganic nanoparticles having surface charges opposed to those of the charges of the polyelectrolyte of step (c) to the charged polyelectrolyte deposit, followed by at least one washing step to form at least one layer of ionised inorganic nanoparticles; removal of the deposit of polymer microparticles of step (b) and the deposits of polyelectrolyte of steps (a) and (c) to obtain the deposit of inorganic nanoparticles having micro gaps.
摘要翻译： 本发明涉及一种生产覆盖有无机纳米颗粒沉积物的透明支撑体的方法，所述沉积物具有微隙，包括以下步骤：将具有电离官能团的聚电解质溶液施加到透明载体上，随后在 至少一个洗涤和干燥步骤以在所述载体上形成带电的聚电解质沉积物; 将具有与聚电解质沉积物相反的电荷的电离基团的聚合物微粒溶液施加到所述聚电解质沉积物上，随后至少一个洗涤步骤用于在聚电解质沉积物上形成带电聚合物微粒的沉积物; 用具有电离官能团的聚电解质溶液覆盖带电聚合物微粒的沉积物，其电荷与步骤（b）的带电聚合物微粒的电荷相反，然后进行至少一个洗涤和干燥步骤以形成带电的聚电解质沉积物; 将具有与步骤（c）的聚电解质的电荷相反的表面电荷的电离无机纳米颗粒溶液施加到带电的聚电解质沉积物中，随后进行至少一个洗涤步骤以形成至少一层电离无机纳米颗粒; 去除步骤（b）的聚合物微粒的沉积物和步骤（a）和（c）的聚电解质的沉积物，以获得具有微隙的无机纳米颗粒的沉积。
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