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公开(公告)号：US10564160B2
公开(公告)日：2020-02-18
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/569
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
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公开(公告)号：US4061625A
公开(公告)日：1977-12-06
申请号：US697003
申请日：1976-06-17
IPC分类号： C07K1/06 , C07K5/087 , C07C103/52
CPC分类号： C07K5/0812 , C07K1/064 , C12Q2337/12 , C12Q2337/30 , G01N2333/974 , Y10S530/802
摘要： Substrate with high specificity to thrombin and thrombin-like enzymes having the formula: ##STR1## and salts thereof, wherein R.sub.1 is H or OH; R.sub.2 is nitrophenyl; and n is 1, 2 or 3 provided along with the method of use.
摘要翻译： 对具有下式的凝血酶和凝血酶样酶具有高特异性的底物及其盐，其中R 1是H或OH; R2是硝基苯基; 和n是1,2或3，与使用方法一起提供。
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公开(公告)号：US07820633B2
公开(公告)日：2010-10-26
申请号：US10441779
申请日：2003-05-19
申请人： Lucilla Steinaa , Jesper Haaning , Iben Dalum , Peter Birk , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Anand Gautam , Søren Mouritsen
发明人： Lucilla Steinaa , Jesper Haaning , Iben Dalum , Peter Birk , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Anand Gautam , Søren Mouritsen
IPC分类号： A61K31/70 , A61K31/711 , C12N15/00 , C07H21/04
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
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公开(公告)号：US07807441B2
公开(公告)日：2010-10-05
申请号：US11202516
申请日：2005-08-11
申请人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Haaning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
发明人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Haaning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
IPC分类号： C12N1/00 , C12N1/10 , C12N1/14 , C12N1/16 , C12N1/20 , C12N5/10 , C12N5/14 , C12N5/16 , C12N15/00 , A61K38/00 , C07K14/00
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。
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公开(公告)号：US20110244477A1
公开(公告)日：2011-10-06
申请号：US13061085
申请日：2009-08-28
IPC分类号： G01N33/53 , G01N33/566
CPC分类号： G01N33/56972 , G01N33/6854 , G01N2333/16 , G01N2469/20 , G01N2800/24
摘要： An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.
摘要翻译： 描述了一种改进的测定法，其中表面具有固定化抗Ig抗体而不是抗原，并且使用含有几种可能标记之一的可溶性抗原探针检测特异性抗体分泌细胞（ASC）。 该方法具有更少的背景的改善的灵敏度，并且也更具代表性，因为抗原结合不使用固定的抗原。 该测定对于测量针对HIV的抗体分泌细胞，用于确定感染是否与老的或潜伏期相比是急性的，用于测定表位并测量相同反应中针对不同抗原的ASC的测定。
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公开(公告)号：US07005498B1
公开(公告)日：2006-02-28
申请号：US09806703
申请日：1999-10-05
申请人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
发明人： Lucilla Steinaa , Søren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Gregorius Nielsen , Gunilla Karlsson , Peter Birk Rasmussen
IPC分类号： C07K9/00
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。
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公开(公告)号：US20060008465A1
公开(公告)日：2006-01-12
申请号：US11202516
申请日：2005-08-11
申请人： Lucilla Steinaa , Soren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Nielsen , Gunilla Karlsson , Peter Rasmussen
发明人： Lucilla Steinaa , Soren Mouritsen , Anand Gautam , Iben Dalum , Jesper Hanning , Dana Leach , Klaus Nielsen , Gunilla Karlsson , Peter Rasmussen
CPC分类号： C07K16/3069 , A61K39/0011 , C07K14/50 , C07K14/705 , C07K14/70539 , C07K14/71 , C07K2317/34 , C12N2799/021 , Y02A50/412
摘要： A method is disclosed for inducing cell-mediated immunity against cellular antigens. More specifically, the invention provides for a method for inducing cytotoxic T-lymphocyte immunity against weak antigens, notably self-proteins. The method entails that antigen presenting cells are induced to present at least one CTL epitope of the weak antigen and at the same time presenting at least one foreign T-helper lymphocyte epitope. In a preferred embodiment, the antigen is a cancer specific antigen, e.g. PSM, Her2, or FGF8b. The method can be exercised by using traditional polypeptide vaccination, but also by using live attenuated vaccines or nucleic acid vaccination. The invention furthermore provides immunogenic analogues of PSM, Her2 and FGF8b, as well as nucleic acid molecules encoding these analogues. Also vectors and transformed cells are disclosed. The invention also provides for a method for identification of immunogenic analogues of weak or non-immunogenic antigens.
摘要翻译： 公开了一种用于诱导针对细胞抗原的细胞介导的免疫的方法。 更具体地，本发明提供了一种诱导针对弱抗原，特别是自身蛋白的细胞毒性T淋巴细胞免疫的方法。 该方法需要抗原呈递细胞被诱导以呈现弱抗原的至少一个CTL表位，并且同时呈现至少一种外来T辅助淋巴细胞表位。 在优选的实施方案中，抗原是癌特异性抗原，例如 PSM，Her2或FGF8b。 该方法可以通过使用传统的多肽疫苗接种，也可以通过使用活减毒疫苗或核酸接种来进行。 本发明还提供了PSM，Her2和FGF8b的免疫原性类似物以及编码这些类似物的核酸分子。 还公开了载体和转化的细胞。 本发明还提供了用于鉴定弱或非免疫原性抗原的免疫原性类似物的方法。
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