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公开(公告)号：US5298399A
公开(公告)日：1994-03-29
申请号：US732242
申请日：1991-07-18
申请人： Takeshi Uozumi , Haruhiko Masaki , Makoto Hidaka , Akira Nakamura , Michihisa Maeda , Yasuo Yoneta
发明人： Takeshi Uozumi , Haruhiko Masaki , Makoto Hidaka , Akira Nakamura , Michihisa Maeda , Yasuo Yoneta
CPC分类号： C12N9/80
摘要： An isolated structural Bacillus sp. TB-90 (FERM BP-795) urease gene, which comprises base sequences encoding the amino acid sequences of three subunits of urease. A recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli. A process for producing urease, which comprises cultivating Escherichia coli carrying a recombinant DNA comprising Bacillus sp. TB-90 (FERM BP-795) urease gene capable of replicating in Escherichia coli and recovering urease from the culture mixture
摘要翻译： 分离结构芽孢杆菌 TB-90（FERM BP-795）脲酶基因，其包含编码脲酶的三个亚基的氨基酸序列的碱基序列。 包含芽孢杆菌属的重组DNA 能在大肠杆菌中复制的TB-90（FERM BP-795）脲酶基因。 一种生产脲酶的方法，其包括培养携带包含芽孢杆菌属（Bacillus sp。）的重组DNA的大肠杆菌。 TB-90（FERM BP-795）能够在大肠杆菌中复制的脲酶基因并从培养物中回收尿素酶
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公开(公告)号：US20070243604A1
公开(公告)日：2007-10-18
申请号：US10522366
申请日：2003-07-28
CPC分类号： C12N15/1086 , C12N15/65
摘要： A DNA fragment prepared by inserting a translation termination codon into 5′ upstream side of the active site of a lethal gene, a transformant selection marker which uses the same, and a vector into which the marker is inserted. Since this lethal gene is used as a gene marker, complete extinction of transformants having no exogenous gene can be achieved, and a transformant selection marker capable of effecting stable amplification of a vector containing an exogenous gene in a host can be obtained. In addition, a vector which can effect accurate and efficient gene analyses by DNA microarray and the like is obtained.
摘要翻译： 通过将翻译终止密码子插入到致死基因的活性位点的5'上游侧，使用其的转化体选择标记物和插入标记物的载体制备的DNA片段。 由于该致死基因用作基因标记，因此可以实现无外源基因转化体的完全消光，能够获得能够在宿主中含有外源基因的载体稳定扩增的转化体选择标记。 此外，可以获得通过DNA微阵列等进行准确有效的基因分析的载体。
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