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20 条记录 (耗时 0.026 秒)

    Marker for Selecting Transformant with The Use of Lethal Gene
    1.
    发明申请
    Marker for Selecting Transformant with The Use of Lethal Gene 审中-公开
    使用致命基因选择转化体的标记

    公开(公告)号：US20070243604A1

    公开(公告)日：2007-10-18

    申请号：US10522366

    申请日：2003-07-28

    申请人： Masayuki Machida ,  Haruhiko Masaki ,  Sumiko Kunihiro ,  Hiroko Hagiwara

    发明人： Masayuki Machida ,  Haruhiko Masaki ,  Sumiko Kunihiro ,  Hiroko Hagiwara

    IPC分类号： C12N15/00 ,  C07H21/04

    CPC分类号： C12N15/1086 ,  C12N15/65

    摘要： A DNA fragment prepared by inserting a translation termination codon into 5′ upstream side of the active site of a lethal gene, a transformant selection marker which uses the same, and a vector into which the marker is inserted. Since this lethal gene is used as a gene marker, complete extinction of transformants having no exogenous gene can be achieved, and a transformant selection marker capable of effecting stable amplification of a vector containing an exogenous gene in a host can be obtained. In addition, a vector which can effect accurate and efficient gene analyses by DNA microarray and the like is obtained.

    摘要翻译： 通过将翻译终止密码子插入到致死基因的活性位点的5'上游侧，使用其的转化体选择标记物和插入标记物的载体制备的DNA片段。 由于该致死基因用作基因标记，因此可以实现无外源基因转化体的完全消光，能够获得能够在宿主中含有外源基因的载体稳定扩增的转化体选择标记。 此外，可以获得通过DNA微阵列等进行准确有效的基因分析的载体。

    Nucleic acid library and protein library
    2.
    发明申请
    Nucleic acid library and protein library 审中-公开
    核酸文库和蛋白质文库

    公开(公告)号：US20060099602A1

    公开(公告)日：2006-05-11

    申请号：US10953688

    申请日：2004-09-29

    申请人： Hideji Tajima ,  Masayuki Machida ,  Hiroko Hagiwara ,  Sumiko Kunihiro

    发明人： Hideji Tajima ,  Masayuki Machida ,  Hiroko Hagiwara ,  Sumiko Kunihiro

    IPC分类号： C40B40/08

    CPC分类号： C12N15/1065 ,  C12N15/1086 ,  C12N15/1093

    摘要： In order to achieve an object of providing a nucleic acid library and a protein library capable of supplying plural species of proteins correlated with the nucleic acid coding therefor at once, and analyzing immediately after the supply the plural species of protein in parallel, the present invention provides a nucleic acid library comprising not less than two species of library units present in a state in which they are separated from one another, wherein each library unit comprises a nucleic acid that can express not less than one species of protein in an in vitro transcription/translation system or an in vitro translation system, and a first protein trapper that is set contiguously to the nucleic acid, each library unit is set on the surface of a solid support, and the nucleic acid contained in each library unit is immobilized on the surface of the solid support where each library unit is set, and a protein library obtainable by subjecting at once not less than two species of library units possessed by the nucleic acid library to an in vitro transcription/translation system or an in vitro translation system to express at once the nucleic acids contained in the library units.

    摘要翻译： 为了实现提供能够一次提供与其编码的核酸相关的多种蛋白质的核酸文库和蛋白质文库的目的，并且在供给多种蛋白质之后立即分析本发明 提供了一种核酸文库，其包含不少于两种存在于它们彼此分离的状态的文库单元，其中每个文库单元包含在体外转录中可表达不少于一种蛋白质的核酸 /翻译系统或体外翻译系统，以及与核酸相邻设置的第一蛋白捕获器，每个文库单元设置在固体支持物的表面上，并且每个文库单元中所含的核酸被固定在 设置每个文库单元的固体支持物的表面，以及通过一次不少于两种图书馆进行获得的蛋白质文库 y单位由核酸文库所具有的体外转录/翻译系统或体外翻译系统一次性表达文库单位中所含的核酸。

    TRANSCRIPTION REGULATORY FACTORS FOR MANNANASES OR CELLULASES, AND GENES FOR THE TRANSCRIPTION REGULATORY FACTOR
    7.
    发明申请
    TRANSCRIPTION REGULATORY FACTORS FOR MANNANASES OR CELLULASES, AND GENES FOR THE TRANSCRIPTION REGULATORY FACTOR 有权
    转基因生殖因子及细胞因子的转录因子及转录因子

    公开(公告)号：US20120142051A1

    公开(公告)日：2012-06-07

    申请号：US13202279

    申请日：2010-03-02

    申请人： Masahiro Ogawa ,  Yasuji Koyama ,  Masayuki Machida

    发明人： Masahiro Ogawa ,  Yasuji Koyama ,  Masayuki Machida

    IPC分类号： C07K14/38 ,  C12N15/63 ,  C12N9/24 ,  C12N1/15 ,  C12N1/21 ,  C12P21/02 ,  C12N15/31 ,  C12N1/19

    CPC分类号： C12N9/2434 ,  C07K14/38 ,  C12N9/2451 ,  C12N9/2488

    摘要： Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.

    摘要翻译： 公开的是：能够调节甘露聚糖酶或纤维素酶基因的转录或表达的转录调节因子，如下所述; 和别的。 具体公开的是选自以下蛋白质（a），（b）和（c）的蛋白质：（a）包含SEQ ID NO：2所示氨基酸序列的蛋白质; （b）包含在SEQ ID NO：2所示的氨基酸序列中缺失，取代或添加一个或几个氨基酸残基（例如1至5个氨基酸残基）而产生的氨基酸序列的蛋白质，其能够 调节甘露聚糖酶或纤维素酶基因的转录; 和（c）蛋白质，其包含与SEQ ID NO：2所示的氨基酸序列具有70％或更高序列同一性的氨基酸序列，并且能够调节甘露聚糖酶或纤维素酶的基因的转录，或部分 蛋白质的片段。 还具体公开了编码蛋白质的基因等。

    Method of degrading plastic and process for producing useful substance using the same
    10.
    发明申请
    Method of degrading plastic and process for producing useful substance using the same 审中-公开
    降解塑料的方法和使用其制造有用物质的方法

    公开(公告)号：US20060106120A1

    公开(公告)日：2006-05-18

    申请号：US10532423

    申请日：2003-09-17

    申请人： Keietsu Abe ,  Katsuya Gomi ,  Yohei Yamagata ,  Fumihiko Hasegawa ,  Hiroshi Maeda ,  Tasuku Nakajima ,  Masayuki Machida

    发明人： Keietsu Abe ,  Katsuya Gomi ,  Yohei Yamagata ,  Fumihiko Hasegawa ,  Hiroshi Maeda ,  Tasuku Nakajima ,  Masayuki Machida

    IPC分类号： C08J11/04

    CPC分类号： C12N9/242 ,  B29B17/00 ,  C07K14/375 ,  C08J11/105 ,  C08J2367/02 ,  C08J2367/04 ,  C12N9/18 ,  Y02W30/62 ,  Y02W30/702

    摘要： A method of degrading a plastic in the presence of a biosurfactant; a method of degrading a plastic by contacting the plastic with a microorganism; a process for producing a useful substance from a plastic which comprises degrading the plastic by contacting the plastic with a microorganism and further converting the components of the thus degraded plastic with the use of a microorganism; a method of degrading a plastic by contacting the plastic with a microorganism in the coexistence of a biosurfactant and/or a plastic-degrading enzyme and thus degrading the plastic under the action of the microorganism; a transformant microorganism having been recombined with at least one DNA selected from among a DNA containing a gene encoding a surface active substance, a DNA containing a gene encoding a plastic-degrading enzyme and a DNA containing a gene encoding a useful substance; novel genes as described above; and proteins encoded thereby.

    摘要翻译： 在生物表面活性剂存在下降解塑料的方法; 通过使塑料与微生物接触来降解塑料的方法; 一种从塑料制造有用物质的方法，包括通过使塑料与微生物接触来降解塑料，并且通过使用微生物进一步转化由此降解的塑料的组分; 通过在生物表面活性剂和/或塑性降解酶的共存下使塑料与微生物接触从而在微生物的作用下降解塑料来降解塑料的方法; 已经与选自包含编码表面活性物质的DNA的DNA，含有编码塑性降解酶的基因的DNA和含有编码有用物质的基因的DNA重组的转化体微生物; 如上所述的新基因; 和由此编码的蛋白质。