公开(公告)号：US09063343B2

公开(公告)日：2015-06-23

申请号：US13351666

申请日：2012-01-17

申请人： Masato Nakagawa ,  Hirotada Watanabe ,  Yoichi Wada

发明人： Masato Nakagawa ,  Hirotada Watanabe ,  Yoichi Wada

IPC分类号： G06K9/00 ,  G02B21/36 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  G02B21/14

CPC分类号： G02B21/367 ,  C12M41/14 ,  C12M41/36 ,  C12M41/48 ,  G02B21/14 ,  G06K9/0014

摘要： An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.

摘要翻译： 一种图像处理装置包括：轮廓提取处理单元，输入由观察单元获取的细胞集落的相位对比图像，并提取细胞集落的轮廓;提取单元，提取细胞集落的轮廓部分的特征量; 在外部的亮度信息和相位对比图像的轮廓内部的亮度信息，以及自动识别单元，基于事先确定的判别式标准自动判断细胞集落是否为iPS细胞集落，特征 数量由提取单元提取。

公开(公告)号：US20120133755A1

公开(公告)日：2012-05-31

申请号：US13251673

申请日：2011-10-03

申请人： Hideo KURODA ,  Hirotada Watanabe ,  Yoichi Wada ,  Yasujiro Kiyota ,  Ryuji Koshiba ,  Takayuki Uozumi

发明人： Hideo KURODA ,  Hirotada Watanabe ,  Yoichi Wada ,  Yasujiro Kiyota ,  Ryuji Koshiba ,  Takayuki Uozumi

IPC分类号： H04N7/18

CPC分类号： G02B21/367 ,  G01N21/6486 ,  G02B21/16 ,  G02B21/365 ,  G06K9/00134 ,  G06K9/0014

摘要： A biological specimen observation apparatus whereby observation of a biological specimen can be performed accurately. In macro observation, a biological change region is extracted from a macro image, a micro observation point corresponding to an extracted biological change region is registered, and an object for tracking is identified. In micro observation, it is judged from the micro image whether or not biological change has continued in the biological change region at the micro observation point, and the registered micro observation point is updated on the basis of this judgment result. It is possible to carry out both macro observation for detecting a biological change region and micro observation for observing the progress of growth of a partial minute region where biological change has been exhibited, and to carry out accurate observation of a biological specimen.

摘要翻译： 可以准确地进行生物样本的观察的生物样本观察装置。 在宏观观察中，从宏观图像提取生物变化区域，登记与提取的生物体变化区域对应的微观观察点，并且识别跟踪对象。 在微观观察中，从微观图像判断微观察点处的生物变化区域的生物变化是否持续，并且基于该判断结果来更新登记的微观察点。 可以进行用于检测生物变化区域的宏观观察和用于观察已经显示生物学变化的部分微小区域的生长进展的微观察，并进行生物样本的准确观察。

公开(公告)号：US20120114219A1

公开(公告)日：2012-05-10

申请号：US13351666

申请日：2012-01-17

申请人： Masato NAKAGAWA ,  Hirotada WATANABE ,  Yoichi WADA

发明人： Masato NAKAGAWA ,  Hirotada WATANABE ,  Yoichi WADA

IPC分类号： G06K9/46

CPC分类号： G02B21/367 ,  C12M41/14 ,  C12M41/36 ,  C12M41/48 ,  G02B21/14 ,  G06K9/0014

摘要： An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.

摘要翻译： 一种图像处理装置包括：轮廓提取处理单元，输入由观察单元获取的细胞集落的相位对比图像，并提取细胞集落的轮廓;提取单元，提取细胞集落的轮廓部分的特征量; 在外部的亮度信息和相位对比图像的轮廓内部的亮度信息，以及自动识别单元，基于事先确定的判别式标准自动判断细胞集落是否为iPS细胞集落，特征 数量由提取单元提取。

公开(公告)号：US09442282B2

公开(公告)日：2016-09-13

申请号：US13251673

申请日：2011-10-03

申请人： Hideo Kuroda ,  Hirotada Watanabe ,  Yoichi Wada ,  Yasujiro Kiyota ,  Ryuji Koshiba ,  Takayuki Uozumi

发明人： Hideo Kuroda ,  Hirotada Watanabe ,  Yoichi Wada ,  Yasujiro Kiyota ,  Ryuji Koshiba ,  Takayuki Uozumi

IPC分类号： G02B21/36 ,  G01N21/64 ,  G02B21/16

CPC分类号： G02B21/367 ,  G01N21/6486 ,  G02B21/16 ,  G02B21/365 ,  G06K9/00134 ,  G06K9/0014

摘要： A biological specimen observation apparatus whereby observation of a biological specimen can be performed accurately. In macro observation, a biological change region is extracted from a macro image, a micro observation point corresponding to an extracted biological change region is registered, and an object for tracking is identified. In micro observation, it is judged from the micro image whether or not biological change has continued in the biological change region at the micro observation point, and the registered micro observation point is updated on the basis of this judgment result. It is possible to carry out both macro observation for detecting a biological change region and micro observation for observing the progress of growth of a partial minute region where biological change has been exhibited, and to carry out accurate observation of a biological specimen.

摘要翻译： 可以准确地进行生物样本的观察的生物样本观察装置。 在宏观观察中，从宏观图像提取生物变化区域，登记与提取的生物体变化区域对应的微观观察点，并且识别跟踪对象。 在微观观察中，从微观图像判断微观察点处的生物变化区域的生物变化是否持续，并且基于该判断结果来更新登记的微观察点。 可以进行用于检测生物变化区域的宏观观察和用于观察已经显示生物学变化的部分微小区域的生长进展的微观察，并进行生物样本的准确观察。