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公开(公告)号：US09758765B2
公开(公告)日：2017-09-12
申请号：US14110063
申请日：2012-04-09
CPC分类号： C12N5/0696 , C07K14/78 , C07K2319/02 , C07K2319/21 , C07K2319/42 , C07K2319/43
摘要： Provided are a modified laminin having a cell-growth regulatory molecule bound to at least one site selected from the α chain N-terminus, the α chain C-terminus, the β chain N-terminus and the γ chain N-terminus of laminin or a heterotrimeric laminin fragment, a method for culturing cells in the presence of the modified laminin, a method for establishing iPS cells in the presence of the modified laminin, and a culture substrate coated with the modified laminin. Human stem cells cultured in a xeno-free environment with the use of the modified laminin of the present invention can be provided as highly safe human stem cells applicable to regenerative medicine.
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2.发明授权公开(公告)号：US09005967B2
公开(公告)日：2015-04-14
申请号：US13497044
申请日：2011-01-21
申请人： Shinya Yamanaka , Masato Nakagawa
发明人： Shinya Yamanaka , Masato Nakagawa
IPC分类号： C12N5/02 , C07K14/435 , C12N5/074
CPC分类号： C07K14/435 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608
摘要： The present invention provides a method for improving iPS cell generation efficiency, which comprises a step of introducing a Myc variant having the following features: (1) having an activity to improve iPS cell generation efficiency which is comparative to, or greater than that of c-Myc; and (2) having a transformation activity which is lower than that of c-Myc; or a nucleic acid encoding the variant, in a nuclear reprogramming step. Also, the present invention provides a method for preparing iPS cells, which comprises a step of introducing the above Myc variant or a nucleic acid encoding the variant and a combination of nuclear reprogramming factors into somatic cells. Moreover, the present invention provides iPS cells comprising the nucleic acid encoding the Myc variant which can be obtained by the above method, and a method for preparing somatic cells which comprises inducing differentiation of the iPS cells.
摘要翻译： 本发明提供了一种提高iPS细胞产生效率的方法，其包括引入具有以下特征的Myc变体的步骤：（1）具有提高iPS细胞产生效率的活性，其与c的比较或大于 -我的C; 和（2）具有低于c-Myc的转化活性的转化活性; 或编码该变体的核酸，在核重编程步骤中。 此外，本发明提供了一种制备iPS细胞的方法，其包括将上述Myc变体或编码变体的核酸和核重编程因子的组合引入体细胞的步骤。 此外，本发明提供了包含编码可以通过上述方法获得的Myc变体的核酸的iPS细胞，以及包含诱导iPS细胞分化的体细胞的制备方法。
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公开(公告)号：US20140127806A1
公开(公告)日：2014-05-08
申请号：US14110063
申请日：2012-04-09
CPC分类号： C12N5/0696 , C07K14/78 , C07K2319/02 , C07K2319/21 , C07K2319/42 , C07K2319/43
摘要： Provided are a modified laminin having a cell-growth regulatory molecule bound to at least one site selected from the α chain N-terminus, the α chain C-terminus, the β chain N-terminus and the δ chain N-terminus of laminin or a heterotrimeric laminin fragment, a method for culturing cells in the presence of the modified laminin, a method for establishing iPS cells in the presence of the modified laminin, and a culture substrate coated with the modified laminin. Human stem cells cultured in a xeno-free environment with the use of the modified laminin of the present invention can be provided as highly safe human stem cells applicable to regenerative medicine.
摘要翻译： 提供了具有细胞生长调节分子的修饰的层粘连蛋白，其结合至至少一个选自α链N末端，α链C末端， 连锁N末端和层粘连蛋白的δ链N末端或异源三聚体层粘连蛋白片段，在修饰层粘连蛋白存在下培养细胞的方法，在修饰层粘连蛋白存在下建立iPS细胞的方法和培养基质 涂上改性层粘连蛋白。 使用本发明的改性层粘连蛋白在无异种环境中培养的人类干细胞可以被提供为适用于再生医学的高度安全的人类干细胞。
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公开(公告)号：US20120196360A1
公开(公告)日：2012-08-02
申请号：US13389359
申请日：2010-08-06
申请人： Keisuke Okita , Masato Nakagawa , Shinya Yamanaka
发明人： Keisuke Okita , Masato Nakagawa , Shinya Yamanaka
CPC分类号： C12N15/85 , C12N5/0696 , C12N2501/40 , C12N2501/48 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/608 , C12N2502/13 , C12N2506/1361 , C12N2510/00 , C12N2800/108 , C12N2800/30
摘要： Provided is a method of producing an iPS cell, comprising bringing (a) Oct3/4 or a nucleic acid that encodes the same, (b) Klf4 or a nucleic acid that encodes the same, and (c) Sox2 or a nucleic acid that encodes the same, as well as (d1) L-Myc or a nucleic acid that encodes the same and/or (d2) a functional inhibitor of p53, into contact with a somatic cell. It is preferable that (a) a nucleic acid that encodes Oct3/4, (b) a nucleic acid that encodes Klf4, (c) a nucleic acid that encodes Sox2, (d1) a nucleic acid that encodes L-Myc and (e) a nucleic acid that encodes Lin28 or Lin28b be inserted into an episomal vector having loxP sequences placed in the same orientation on the 5′ and 3′ sides of a vector constituent essential for the replication of the vector, that (d2) a nucleic acid that encodes an shRNA against p53 be inserted into a vector ensuring transient expression (plasmid vector and the like), and that all these nucleic acids be transferred to a somatic cell.
摘要翻译： 本发明提供一种iPS细胞的制造方法，其特征在于：（a）Oct3 / 4或其编码的核酸，（b）Klf4或其编码的核酸，以及（c）Sox2或所述核酸， 编码相同的，以及（d1）L-Myc或编码与p53的功能性抑制剂相同和/或（d2）的核酸与体细胞接触。 优选（a）编码Oct3 / 4的核酸，（b）编码Klf4的核酸，（c）编码Sox2的核酸，（d1）编码L-Myc的核酸和（e ）将编码Lin28或Lin28b的核酸插入具有放置在与载体复制必需的载体成分的5'和3'侧相同取向上的loxP序列的附加型载体中，（d2）核酸 将编码针对p53的shRNA插入到确保瞬时表达（质粒载体等）的载体中，并将所有这些核酸转移到体细胞。
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公开(公告)号：US06237570B1
公开(公告)日：2001-05-29
申请号：US09166576
申请日：1998-10-06
申请人： Hiromasa Aoki , Hisaharu Takeuchi , Ken Uchiyama , Masato Nakagawa , Yukinori Miyata , Shuichi Matsumoto , Masatoshi Kuroyanagi , Shinji Ueda
发明人： Hiromasa Aoki , Hisaharu Takeuchi , Ken Uchiyama , Masato Nakagawa , Yukinori Miyata , Shuichi Matsumoto , Masatoshi Kuroyanagi , Shinji Ueda
IPC分类号： F02M3704
CPC分类号： F02M61/165 , F02M47/027 , F02M55/002 , F02M55/007 , F02M55/04 , F02M63/0017 , F02M63/0225 , F02M2200/315
摘要： Pressurized fuel of a common rail is introduced into a control chamber of a fuel injector. An electromagnetic valve opens or closes a fuel discharge passage of the control chamber to adjust a hydraulic pressure of the control chamber. Through a switching leak passage, bubble-containing fuel directly returns from a valve opening of the electromagnetic valve to a low-pressure return passage without passing through an armature chamber. Through a stationary leak passage, the fuel leaking from every slide portion returns to the return passage via the armature chamber. A downstream portion of the stationary leak passage opens to the upper portion of the armature chamber. A damper element is provided downstream of the electromagnetic valve in the return passage for canceling an increased fuel pressure.
摘要翻译： 共轨的加压燃料被引入到燃料喷射器的控制室中。 电磁阀打开或关闭控制室的燃料排放通道，以调节控制室的液压。 通过切换泄漏通道，含气体的燃料直接从电磁阀的阀口返回到低压返回通道，而不通过衔铁室。 通过固定的泄漏通道，从每个滑动部分泄漏的燃料经由电枢室返回到返回通道。 固定泄漏通道的下游部分通向衔铁室的上部。 在返回通道中的电磁阀的下游设置阻尼元件，用于消除增加的燃料压力。
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6.发明授权Image processing apparatus, incubation observing apparatus, and image processing method 有权图像处理装置，孵化观察装置和图像处理方法公开(公告)号：US09063343B2
公开(公告)日：2015-06-23
申请号：US13351666
申请日：2012-01-17
申请人： Masato Nakagawa , Hirotada Watanabe , Yoichi Wada
发明人： Masato Nakagawa , Hirotada Watanabe , Yoichi Wada
CPC分类号： G02B21/367 , C12M41/14 , C12M41/36 , C12M41/48 , G02B21/14 , G06K9/0014
摘要： An image processing apparatus includes an outline extracting processing unit inputting a phase contrast image of a cell colony acquired by an observing unit, and extracting an outline of the cell colony, an extracting unit extracting a feature quantity of an outline part of the cell colony based on brightness information at an outside and brightness information at an inside of the outline on the phase contrast image, and an automatic discriminating unit automatically discriminating whether or not the cell colony is an iPS cell colony based on a discriminant criterion determined in advance and the feature quantity extracted by the extracting unit.
摘要翻译： 一种图像处理装置包括：轮廓提取处理单元，输入由观察单元获取的细胞集落的相位对比图像，并提取细胞集落的轮廓;提取单元，提取细胞集落的轮廓部分的特征量; 在外部的亮度信息和相位对比图像的轮廓内部的亮度信息，以及自动识别单元，基于事先确定的判别式标准自动判断细胞集落是否为iPS细胞集落，特征 数量由提取单元提取。
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公开(公告)号：US20120276636A1
公开(公告)日：2012-11-01
申请号：US13497044
申请日：2011-01-21
申请人： Shinya Yamanaka , Masato Nakagawa
发明人： Shinya Yamanaka , Masato Nakagawa
CPC分类号： C07K14/435 , C12N5/0696 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608
摘要： The present invention provides a method for improving iPS cell generation efficiency, which comprises a step of introducing a Myc variant having the following features: (1) having an activity to improve iPS cell generation efficiency which is comparative to, or greater than that of c-Myc; and (2) having a transformation activity which is lower than that of c-Myc; or a nucleic acid encoding the variant, in a nuclear reprogramming step. Also, the present invention provides a method for preparing iPS cells, which comprises a step of introducing the above Myc variant or a nucleic acid encoding the variant and a combination of nuclear reprogramming factors into somatic cells. Moreover, the present invention provides iPS cells comprising the nucleic acid encoding the Myc variant which can be obtained by the above method, and a method for preparing somatic cells which comprises inducing differentiation of the iPS cells.
摘要翻译： 本发明提供了一种提高iPS细胞产生效率的方法，其包括引入具有以下特征的Myc变体的步骤：（1）具有提高iPS细胞产生效率的活性，其与c的比较或大于 -我的C; 和（2）具有低于c-Myc的转化活性的转化活性; 或编码该变体的核酸，在核重编程步骤中。 此外，本发明提供了一种制备iPS细胞的方法，其包括将上述Myc变体或编码变体的核酸和核重编程因子的组合引入体细胞的步骤。 此外，本发明提供了包含编码可以通过上述方法获得的Myc变体的核酸的iPS细胞，以及包含诱导iPS细胞分化的体细胞的制备方法。
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公开(公告)号：US08166337B2
公开(公告)日：2012-04-24
申请号：US12230241
申请日：2008-08-26
申请人： Masato Nakagawa
发明人： Masato Nakagawa
IPC分类号： G06F11/00
CPC分类号： G06F11/2268 , G06F11/079
摘要： Relating with board numbers of the boards mounted with the logic circuits and mounted places on the boards and in relation to log information to be collected from the logic circuits, analysis information describing information to be processed when the log information is generated, information of a condition for which the log information is to be valid, and information of a condition for which the log information is to be invalid are defined for analyzing failures using the analysis information based on the logic circuits. Upon the realization of the failure analysis based on the logic circuits, the analysis information further describes information of the priority of the log information to realize a thorough analysis of critical failures.
摘要翻译： 关于安装有逻辑电路的板的板号和板上的安装位置以及从逻辑电路收集的日志信息，描述当生成日志信息时要处理的信息的分析信息，条件信息 定义日志信息有效的日志信息的信息和日志信息无效的条件的信息，用于基于逻辑电路使用分析信息来分析故障。 在实现基于逻辑电路的故障分析时，分析信息进一步描述了日志信息的优先级信息，以实现关键故障的彻底分析。
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公开(公告)号：US20090006896A1
公开(公告)日：2009-01-01
申请号：US12230241
申请日：2008-08-26
申请人： Masato Nakagawa
发明人： Masato Nakagawa
IPC分类号： G06F11/07
CPC分类号： G06F11/2268 , G06F11/079
摘要： Relating with board numbers of the boards mounted with the logic circuits and mounted places on the boards and in relation to log information to be collected from the logic circuits, analysis information describing information to be processed when the log information is generated, information of a condition for which the log information is to be valid, and information of a condition for which the log information is to be invalid are defined for analyzing failures using the analysis information based on the logic circuits. Upon the realization of the failure analysis based on the logic circuits, the analysis information further describes information of the priority of the log information to realize a thorough analysis of critical failures.
摘要翻译： 关于安装有逻辑电路的板的板号和板上的安装位置以及从逻辑电路收集的日志信息，描述当生成日志信息时要处理的信息的分析信息，条件信息 定义日志信息有效的日志信息的信息和日志信息无效的条件的信息，用于基于逻辑电路使用分析信息来分析故障。 在实现基于逻辑电路的故障分析时，分析信息进一步描述了日志信息的优先级信息，以实现关键故障的彻底分析。
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公开(公告)号：US5802954A
公开(公告)日：1998-09-08
申请号：US617292
申请日：1996-03-18
申请人： Hayato Ikeda , Hiromi Michiyuki , Masanobu Yokoi , Yasunori Ueda , Masato Nakagawa , Manabu Naoi
发明人： Hayato Ikeda , Hiromi Michiyuki , Masanobu Yokoi , Yasunori Ueda , Masato Nakagawa , Manabu Naoi
CPC分类号： F04B27/1063
摘要： A reciprocating piston type compressor for compressing refrigerant gas is provided with a main housing which includes a plurality of parallel cylinder bores arranged around the longitudinal axis of the cylinder block. The main housing has first and second cylinder blocks connected to each other by a plurality of bolts, the cylinder blocks including inner clamping faces and abutting faces. A plurality of pistons are slidably provided within the cylinder bores. An axially extending drive shaft is supported by the main housing for rotation through a pair of bearings. A swash plate is mounted on the drive shaft for rotation with the drive shaft. A pair of thrust bearings are provided between the swash plate and the inner clamping faces of the cylinder blocks to clamp and hold the swash plate therebetween. The clamping force on the thrust bearings from the inner clamping faces is controlled so that the clamping force does not exceed a predetermined allowable upper limit when the compressor is assembled.
摘要翻译： 一种用于压缩制冷剂气体的往复活塞式压缩机设置有主壳体，该主壳体包括围绕气缸体的纵向轴线布置的多个平行的气缸孔。 主壳体具有通过多个螺栓彼此连接的第一和第二气缸体，气缸体包括内夹紧面和邻接面。 多个活塞可滑动地设置在气缸孔内。 轴向延伸的驱动轴由主壳体支撑以通过一对轴承旋转。 旋转斜盘安装在驱动轴上以与驱动轴一起旋转。 一对推力轴承设置在旋转斜盘和气缸体的内夹紧面之间，以夹紧和保持其间的斜盘。 控制来自内夹紧面的推力轴承上的夹紧力，使得当组装压缩机时夹紧力不超过预定的允许上限。
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