公开(公告)号：US08338643B2

公开(公告)日：2012-12-25

申请号：US12312648

申请日：2007-11-26

申请人： Shinji Takeoka ,  Naoya Takeda ,  Hitoshi Kurumizaka ,  Isao Sakane ,  Namiko Ikegaya ,  Yosuke Obata ,  Syunsuke Saito

发明人： Shinji Takeoka ,  Naoya Takeda ,  Hitoshi Kurumizaka ,  Isao Sakane ,  Namiko Ikegaya ,  Yosuke Obata ,  Syunsuke Saito

IPC分类号： C07C251/00 ,  C07C233/00 ,  C07C253/00 ,  C07C237/00 ,  C07C239/00 ,  C07C321/00 ,  C07C323/00 ,  C07C381/00

CPC分类号： C07C237/22 ,  C07C237/08 ,  C07C237/12 ,  C07K5/021 ,  C07K5/06086 ,  C07K5/0815

摘要： The present invention provides a reagent for introducing a protein or gene into a cell. The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (wherein in formula (I)-1: L is a single bond, —CONH—, or —S—S—; M1 is —(CH2)k— or —(CH2CH2O)k— (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).

摘要翻译： 本发明提供了将蛋白质或基因导入细胞的试剂。 本发明的试剂是例如将蛋白质或基因导入细胞的试剂，其包含含有下式（I）-1表示的阳离子型氨基酸型脂质的组合物（式中，式 I）-1：L是单键，-CONH-或-S-S-; M 1是 - （CH 2）k - 或 - （CH 2 CH 2 O）k-（其中k是0和14之间的整数）;和 m1和m2各自独立地为11-21之间的整数（在这方面，当提供用于将基因导入细胞的试剂时，m1和m2均为15的情况除外））。

公开(公告)号：US20100291672A1

公开(公告)日：2010-11-18

申请号：US12312648

申请日：2007-11-26

申请人： Shinji Takeoka ,  Naoya Takeda ,  Hitoshi Kurumizaka ,  Isao Sakane ,  Namiko Ikegaya ,  Yosuke Obata ,  Syunsuke Saito

发明人： Shinji Takeoka ,  Naoya Takeda ,  Hitoshi Kurumizaka ,  Isao Sakane ,  Namiko Ikegaya ,  Yosuke Obata ,  Syunsuke Saito

IPC分类号： C12N5/07 ,  C07C229/26

CPC分类号： C07C237/22 ,  C07C237/08 ,  C07C237/12 ,  C07K5/021 ,  C07K5/06086 ,  C07K5/0815

摘要： The present invention provides a reagent for introducing a protein or gene into a cell. The reagent of the present invention is, for example, a reagent for introducing a protein or gene into a cell, which comprises a composition comprising a cationic amino acid type lipid represented by the following formula (I)-1: (wherein in formula (I)-1: L is a single bond, —CONH—, or —S—S—; M1 is —(CH2)k— or —(CH2CH2O)k— (wherein k is an integer between 0 and 14); and m1 and m2 are each independently an integer between 11 and 21 (in this regard, when providing a reagent for introducing a gene into a cell, the case where both m1 and m2 are 15 is excluded)).

摘要翻译： 本发明提供了将蛋白质或基因导入细胞的试剂。 本发明的试剂是例如将蛋白质或基因导入细胞的试剂，其包含含有下式（I）-1表示的阳离子型氨基酸型脂质的组合物（式中，式 I）-1：L是单键，-CONH-或-S-S-; M 1是 - （CH 2）k - 或 - （CH 2 CH 2 O）k-（其中k是0和14之间的整数）;和 m1和m2各自独立地为11-21之间的整数（在这方面，当提供用于将基因导入细胞的试剂时，m1和m2均为15的情况除外））。

公开(公告)号：US20100068766A1

公开(公告)日：2010-03-18

申请号：US12529616

申请日：2008-03-07

申请人： Hitoshi Kurumizaka ,  Motoki Takaku ,  Shinichi Machida

发明人： Hitoshi Kurumizaka ,  Motoki Takaku ,  Shinichi Machida

IPC分类号： C12P19/34 ,  C12N9/90 ,  C07H21/04 ,  C12N9/99

CPC分类号： C12N9/22 ,  C12N15/10

摘要： It is an object of the present invention to provide a composition for catalyzing the cleavage of a single-stranded DNA and the binding of such single-stranded DNA. The present invention provides a composition for cleaving a single-stranded DNA and/or binding the 5′-terminus of such single-stranded DNA to the 3′-terminus thereof, which comprises an Ev1 protein. Moreover, the present invention also provides a composition for cleaving a single-stranded DNA and/or binding the 5′-terminus of such single-stranded DNA to the 3′-terminus thereof, which further comprises a Rad51B protein and/or a DNA topoisomerase type I protein, as well as the Ev1 protein.

摘要翻译： 本发明的目的是提供用于催化单链DNA的切割和这种单链DNA的结合的组合物。 本发明提供了包含Ev1蛋白的用于将单链DNA切割和/或结合这样的单链DNA的5'末端的3'末端的组合物。 此外，本发明还提供了用于将单链DNA切割和/或将这种单链DNA的5'末端与其3'末端结合的组合物，其还包含Rad51B蛋白和/或DNA 拓扑异构酶I型蛋白，以及Ev1蛋白。

公开(公告)号：US20070031849A1

公开(公告)日：2007-02-08

申请号：US10838572

申请日：2004-05-05

申请人： Hitoshi Kurumizaka ,  Wataru Kagawa ,  Shigeyuki Yokoyama

发明人： Hitoshi Kurumizaka ,  Wataru Kagawa ,  Shigeyuki Yokoyama

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/22

CPC分类号： C12Q1/25 ,  C07K2299/00 ,  G01N33/6803 ,  G01N2500/00 ,  G16B15/00

摘要： A crystal of DNA recombination/repair protein, Rad52 protein is prepared and the three-dimensional structure thereof is determined by X-ray crystallography with the use of the same. The obtained three-dimensional structure of Rad52 protein can be used for screening a variety of mutant enzymes or compounds which binds to the enzyme complex. The mutant Rad52 protein complex, or the compound that binds to the complex so as to modulate the DNA recombinational repairing activity, which are screened by the method of the present invention, can be used for diagnosis and treatment of diseases induced by DNA damages, and detection of the DNA recombinational repairing activity in vivo. Moreover, these substances are usable in drug discovery targeting to Rad52, and gene transfer to a specific site by homologous recombination, etc.

摘要翻译： 制备了DNA重组/修复蛋白Rad52蛋白的晶体，并利用X射线晶体学确定其三维结构。 获得的Rad52蛋白的三维结构可用于筛选与酶复合物结合的各种突变酶或化合物。 通过本发明的方法筛选的突变型Rad52蛋白复合物或与复合物结合以调节DNA重组修复活性的化合物可用于诊断和治疗由DNA损伤引起的疾病，以及 检测体内DNA重组修复活性。 此外，这些物质可用于针对Rad52的药物发现，通过同源重组等将基因转移到特定位点。