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公开(公告)号：US07157571B2
公开(公告)日：2007-01-02
申请号：US11030110
申请日：2005-01-07
Applicant: Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin , Mei-Hwei Fan-Chiang
Inventor： Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin , Mei-Hwei Fan-Chiang
CPC classification number: G01N33/5067 , C12N15/85 , C12N2830/008 , C12N2830/85 , G01N2800/52
Abstract: The present invention relates to a chimeric regulatory sequence with hepatoma cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer of 5′ flanking region of human α-fetoprotein (AFP) gene.
Abstract translation: 本发明涉及具有肝癌细胞特异性的嵌合调节序列。 嵌合调节序列包括近端调节序列和人甲胎蛋白（AFP）基因的5'侧翼区的远端增强子。
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公开(公告)号：US20070128722A1
公开(公告)日：2007-06-07
申请号：US11293991
申请日：2005-12-05
Applicant: Pei-Ju Lin , Cheng-Yi Wu , Hui-Ti Lin , Wannhsin Chen
Inventor： Pei-Ju Lin , Cheng-Yi Wu , Hui-Ti Lin , Wannhsin Chen
IPC: C12N5/08
CPC classification number: C12N5/0665 , C12N5/0663 , C12N2502/13
Abstract: Provided herein are methods of proliferating human mesenchymal stem cells obtained from human cord blood and/or human bone marrow aspirates comprising culturing the human mesenchymal stem cells in an environment containing extracellular matrix isolated form human fibroblasts.
Abstract translation: 本文提供了从人脐带血和/或人骨髓抽吸物获得的增殖人间充质干细胞的方法，包括在分离自人成纤维细胞的细胞外基质的环境中培养人间充质干细胞。
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Patent Agency Ranking
公开(公告)号：US20070072172A1
公开(公告)日：2007-03-29
申请号：US11600796
申请日：2006-11-17
Applicant: Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin
Inventor： Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin
CPC classification number: G01N33/5067 , C12N15/85 , C12N2830/008 , C12N2830/85 , G01N2800/52
Abstract: The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer 5 flanking region of human α-fetoprotein (AFP) gene. The chimeric regulatory sequence is useful in purified specific lineages, such as liver cells, from other cell lineages.
Abstract translation: 本发明涉及具有肝细胞特异性的嵌合调节序列。 嵌合调节序列包括近端调节序列和人甲胎蛋白（AFP）基因的侧翼区域的远端增强子5。 嵌合调节序列可用于来自其他细胞谱系的纯化特异谱系，如肝细胞。
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公开(公告)号：US20050158852A1
公开(公告)日：2005-07-21
申请号：US11008929
申请日：2004-12-13
Applicant: Mei-Chih Wang , Mei-Hwei Fan-Chiang , Hui-Ti Lin , Chin-Yu Lin
Inventor： Mei-Chih Wang , Mei-Hwei Fan-Chiang , Hui-Ti Lin , Chin-Yu Lin
IPC: C12N5/0735 , C12N5/08
CPC classification number: C12N5/0606 , C12N2501/70 , C12N2502/13 , C12N2502/1323 , C12N2510/00 , C12N2533/50 , C12N2533/52 , C12N2533/54
Abstract: The present invention discloses a method for improving growth and survival of single human embryonic stem cells. The method includes the step of obtaining a single undifferentiated HES cell; mixing the single undifferentiated cell with an extracellular matrix (ECM) to encompass the cell; and inoculating the mixture onto feeder cells with a nutrient medium in a growth environment. Therefore the single cells can survive, proliferate and grow in vitro.
Abstract translation: 本发明公开了一种改善单人胚胎干细胞生长和存活的方法。 该方法包括获得单个未分化HES细胞的步骤; 将单个未分化细胞与细胞外基质（ECM）混合以包围细胞; 并在生长环境中用营养培养基将混合物接种到饲养细胞上。 因此，单细胞可以在体外存活，增殖和生长。
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公开(公告)号：US07935527B2
公开(公告)日：2011-05-03
申请号：US11008929
申请日：2004-12-13
Applicant: Mei-Chih Wang , Mei-Hwei Fan-Chiang , Hui-Ti Lin , Chin-Yu Lin
Inventor： Mei-Chih Wang , Mei-Hwei Fan-Chiang , Hui-Ti Lin , Chin-Yu Lin
IPC: C12N5/00
CPC classification number: C12N5/0606 , C12N2501/70 , C12N2502/13 , C12N2502/1323 , C12N2510/00 , C12N2533/50 , C12N2533/52 , C12N2533/54
Abstract: The present invention discloses a method for improving growth and survival of single human embryonic stem cells. The method includes the step of obtaining a single undifferentiated HES cell; mixing the single undifferentiated cell with an extracellular matrix (ECM) to encompass the cell; and inoculating the mixture onto feeder cells with a nutrient medium in a growth environment. Therefore the single cells can survive, proliferate and grow in vitro.
Abstract translation: 本发明公开了一种改善单人胚胎干细胞生长和存活的方法。 该方法包括获得单个未分化HES细胞的步骤; 将单个未分化细胞与细胞外基质（ECM）混合以包围细胞; 并在生长环境中用营养培养基将混合物接种到饲养细胞上。 因此，单细胞可以在体外存活，增殖和生长。
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公开(公告)号：US07972851B2
公开(公告)日：2011-07-05
申请号：US11600796
申请日：2006-11-17
Applicant: Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin
Inventor： Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin
CPC classification number: G01N33/5067 , C12N15/85 , C12N2830/008 , C12N2830/85 , G01N2800/52
Abstract: The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer 5′ flanking region of human α-fetoprotein (AFP) gene. The chimeric regulatory sequence is useful in purified specific lineages, such as liver cells, from other cell lineages.
Abstract translation: 本发明涉及具有肝细胞特异性的嵌合调节序列。 嵌合调节序列包括近端调节序列和人甲胎蛋白（AFP）基因的远侧增强子5'侧翼区域。 嵌合调节序列可用于来自其他细胞谱系的纯化特异谱系，如肝细胞。
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公开(公告)号：US20060068370A1
公开(公告)日：2006-03-30
申请号：US11030110
申请日：2005-01-07
Applicant: Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin , Mei-Hwei Fan-Chiang
Inventor： Mei-Chih Wang , Chin-Yu Lin , Hui-Ti Lin , Mei-Hwei Fan-Chiang
CPC classification number: G01N33/5067 , C12N15/85 , C12N2830/008 , C12N2830/85 , G01N2800/52
Abstract: The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer of 5′ flanking region of human α-fetoprotein (AFP) gene. The chimeric regulatory sequence is useful in purified specific cell lineages, such as liver cells, from other cell lineages.
Abstract translation: 本发明涉及具有肝细胞特异性的嵌合调节序列。 嵌合调节序列包括近端调节序列和人甲胎蛋白（AFP）基因的5'侧翼区的远端增强子。 嵌合调节序列可用于来自其他细胞谱系的纯化特异性细胞谱系，例如肝细胞。
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