Method of forming multicellular spheroids from the cultured cells
    1.
    发明申请
    Method of forming multicellular spheroids from the cultured cells 审中-公开
    从培养细胞形成多细胞球体的方法

    公开(公告)号:US20070148767A1

    公开(公告)日:2007-06-28

    申请号:US11318882

    申请日:2005-12-28

    IPC分类号: C12N5/08 C12N5/06

    摘要: Provided herein are methods for producing multicellular spheroids or embryoid bodies suitable for providing cells in large scale for various medical applications. In one embodiment, a method of forming embryoid bodies is provided, which comprises culturing undifferentiated HES cells in a culture vessel pre-coated with cellulose and/or its derivatives. In another embodiment, a method of forming hepatic spheroids is provided, which comprises culturing hepatocytes in a culture vessel pre-coated with cellulose and/or its derivatives.

    摘要翻译: 本文提供了适用于大规模提供用于各种医疗应用的细胞的多细胞球体或胚状体的方法。 在一个实施方案中,提供了形成胚状体的方法,其包括在预先包被纤维素和/或其衍生物的培养容器中培养未分化的HES细胞。 在另一个实施方案中,提供了形成肝球体的方法,其包括在预先涂有纤维素和/或其衍生物的培养容器中培养肝细胞。