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公开(公告)号:US20070148767A1
公开(公告)日:2007-06-28
申请号:US11318882
申请日:2005-12-28
申请人: Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
发明人: Mei-Ju Yang , Wann-Hsin Chen , Pei-Ju Lin , Chun-Hung Chen , Hsing-Wen Sung
CPC分类号: C12N5/0068 , C12N5/0606 , C12N5/067 , C12N2533/78
摘要: Provided herein are methods for producing multicellular spheroids or embryoid bodies suitable for providing cells in large scale for various medical applications. In one embodiment, a method of forming embryoid bodies is provided, which comprises culturing undifferentiated HES cells in a culture vessel pre-coated with cellulose and/or its derivatives. In another embodiment, a method of forming hepatic spheroids is provided, which comprises culturing hepatocytes in a culture vessel pre-coated with cellulose and/or its derivatives.
摘要翻译: 本文提供了适用于大规模提供用于各种医疗应用的细胞的多细胞球体或胚状体的方法。 在一个实施方案中,提供了形成胚状体的方法,其包括在预先包被纤维素和/或其衍生物的培养容器中培养未分化的HES细胞。 在另一个实施方案中,提供了形成肝球体的方法,其包括在预先涂有纤维素和/或其衍生物的培养容器中培养肝细胞。
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公开(公告)号:US20070128722A1
公开(公告)日:2007-06-07
申请号:US11293991
申请日:2005-12-05
申请人: Pei-Ju Lin , Cheng-Yi Wu , Hui-Ti Lin , Wannhsin Chen
发明人: Pei-Ju Lin , Cheng-Yi Wu , Hui-Ti Lin , Wannhsin Chen
IPC分类号: C12N5/08
CPC分类号: C12N5/0665 , C12N5/0663 , C12N2502/13
摘要: Provided herein are methods of proliferating human mesenchymal stem cells obtained from human cord blood and/or human bone marrow aspirates comprising culturing the human mesenchymal stem cells in an environment containing extracellular matrix isolated form human fibroblasts.
摘要翻译: 本文提供了从人脐带血和/或人骨髓抽吸物获得的增殖人间充质干细胞的方法,包括在分离自人成纤维细胞的细胞外基质的环境中培养人间充质干细胞。
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