公开(公告)号：US20120052505A1

公开(公告)日：2012-03-01

申请号：US13214782

申请日：2011-08-22

申请人： Young-Tae CHANG ,  Hyung Ho HA ,  Nam Young KANG ,  Seong Wook YUN ,  Sung Jin PARK ,  Young-Hoon AHN

发明人： Young-Tae CHANG ,  Hyung Ho HA ,  Nam Young KANG ,  Seong Wook YUN ,  Sung Jin PARK ,  Young-Hoon AHN

IPC分类号： G01N21/64 ,  C12Q1/68 ,  C12Q1/42 ,  C12Q1/04

CPC分类号： G01N33/5073

摘要： The present invention relates to methods of detecting, in a sample, embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells. These methods include providing a sample potentially containing such cells and providing a rosamine derivative compound of the formula (I): where the rosamine derivative compound selectively produces fluorescent signals for embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells. These methods also include the steps of contacting the sample with the rosamine derivative compound and detecting the presence of the embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells based on fluorescent signals emitted by the sample following said contacting.

摘要翻译： 本发明涉及在样品中检测胚胎干细胞，诱导多能干细胞和/或经历重编程以产生诱导多能干细胞的细胞的方法。 这些方法包括提供潜在地含有这种细胞的样品并提供式（I）的rosamine衍生物化合物：其中所述rosamine衍生物化合物选择性地产生用于胚胎干细胞，诱导多能干细胞和/或经历重编程产生的细胞的荧光信号 诱导多能干细胞。 这些方法还包括以下步骤：使样品与rosamine衍生物化合物接触并检测胚胎干细胞，诱导的多能干细胞和/或经历重编程的细胞的存在，以基于由样品发射的荧光信号产生诱导多能干细胞 接下来说

公开(公告)号：US08735150B2

公开(公告)日：2014-05-27

申请号：US13214782

申请日：2011-08-22

申请人： Young-Tae Chang ,  Hyung Ho Ha ,  Nam Young Kang ,  Seong Wook Yun ,  Sung Jin Park ,  Young-Hoon Ahn

发明人： Young-Tae Chang ,  Hyung Ho Ha ,  Nam Young Kang ,  Seong Wook Yun ,  Sung Jin Park ,  Young-Hoon Ahn

IPC分类号： C12N5/00 ,  G01N33/567

CPC分类号： G01N33/5073

摘要： The present invention relates to methods of detecting, in a sample, embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells. These methods include providing a sample potentially containing such cells and providing a rosamine derivative compound of the formula (I): where the rosamine derivative compound selectively produces fluorescent signals for embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells. These methods also include the steps of contacting the sample with the rosamine derivative compound and detecting the presence of the embryonic stem cells, induced pluripotent stem cells, and/or cells undergoing reprogramming to produce induced pluripotent stem cells based on fluorescent signals emitted by the sample following said contacting.

摘要翻译： 本发明涉及在样品中检测胚胎干细胞，诱导多能干细胞和/或经历重编程以产生诱导多能干细胞的细胞的方法。 这些方法包括提供潜在地含有这种细胞的样品并提供式（I）的rosamine衍生物化合物：其中所述rosamine衍生物化合物选择性地产生用于胚胎干细胞，诱导多能干细胞和/或经历重编程产生的细胞的荧光信号 诱导多能干细胞。 这些方法还包括以下步骤：使样品与rosamine衍生物化合物接触并检测胚胎干细胞，诱导的多能干细胞和/或经历重编程的细胞的存在，以基于由样品发射的荧光信号产生诱导多能干细胞 接下来说