公开(公告)号：US20180149647A1

公开(公告)日：2018-05-31

申请号：US15830777

申请日：2017-12-04

申请人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

发明人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

IPC分类号： G01N33/561 ,  G01N33/543 ,  G01N27/447 ,  G01N33/53

CPC分类号： G01N33/561 ,  G01N27/447 ,  G01N33/5302 ,  G01N33/54326

摘要： A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

公开(公告)号：US09835623B2

公开(公告)日：2017-12-05

申请号：US14597999

申请日：2015-01-15

申请人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

发明人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

IPC分类号： G01N33/561 ,  G01N33/53 ,  G01N33/543 ,  G01N27/447

CPC分类号： G01N33/561 ,  G01N27/447 ,  G01N33/5302 ,  G01N33/54326

摘要： A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

公开(公告)号：US20150198593A1

公开(公告)日：2015-07-16

申请号：US14597999

申请日：2015-01-15

申请人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

发明人： Andrew Barry ,  Laurel Provencher ,  Seth Cohen ,  I-Jane Chen ,  Jun Yan ,  Jingjing Wang

IPC分类号： G01N33/561

CPC分类号： G01N33/561 ,  G01N27/447 ,  G01N33/5302 ,  G01N33/54326

摘要： A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip; electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region; applying a magnetic field to the magnetic holding region to fix the protein-attached beads in place within the magnetic holding region; binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.

摘要翻译： 一种微流体Western印迹方法和系统，包括用于蛋白质免疫测定的微流体蛋白质印迹法，该方法包括将包含蛋白质的样品引入到芯片上; 电泳分离蛋白质; 将分离的蛋白质与珠粒结合以形成附着蛋白的珠粒，珠粒是磁性的; 将附着蛋白的珠子流入磁性保持区域; 将磁场施加到磁性保持区域以将附着蛋白质的珠子固定在磁性保持区域内的适当位置; 结合初次抗体与蛋白质连接珠上的靶蛋白; 结合第一抗体的结合二抗; 并检测结合的二抗。