公开(公告)号：US07029630B2

公开(公告)日：2006-04-18

申请号：US10879351

申请日：2004-06-30

申请人： Iordan V. Kostov

发明人： Iordan V. Kostov

IPC分类号： G01N21/64

CPC分类号： G01N21/80 ,  G01N21/77 ,  G01N21/84 ,  G01N2021/6439 ,  G01N2021/773 ,  G01N2021/775 ,  G01N2021/7786 ,  G01N2021/7796

摘要： The present invention relates to optical ion sensors, including fluorescence optical ion sensors for use in liquid media in the fields of biology, biotechnology, chemistry, medicine, etc. The present invention provides for optical ion sensors that may be attached to dry hydrophilic or hydrophobic surfaces so as to allow continuous sensing. The optical sensors of the present invention may be sterilized and stored for extended periods of time before use.

公开(公告)号：US07041986B2

公开(公告)日：2006-05-09

申请号：US10389110

申请日：2003-03-14

申请人： Govind Rao ,  Peter Harms ,  Iordan V. Kostov

发明人： Govind Rao ,  Peter Harms ,  Iordan V. Kostov

IPC分类号： G01N21/64

CPC分类号： G01N21/6408 ,  G01N2021/6432

摘要： The present invention provides methods for determining fluorescence lifetime of a fluorophor or an optical sensor using frequency domain gated detection. The method comprises the steps of exciting the fluorophor with frequency modulated light from a pulsed light source, gating a photodetector during excitation of the fluorophore, detecting light emitted from the fluorophor with the photodetector, where the emission light exhibits a phase shift in frequency from that of the excitation light, converting the detected excitation light to an amplified electric signal, and evaluating the amplified electric signal as a measure of fluorescence lifetime of the fluorophor. Also provided is a device with which to use the methods disclosed herein.

公开(公告)号：US07390462B2

公开(公告)日：2008-06-24

申请号：US10609720

申请日：2003-06-30

申请人： Govind Rao ,  Iordan V. Kostov ,  Haley R. Kermis ,  Peter Harms

发明人： Govind Rao ,  Iordan V. Kostov ,  Haley R. Kermis ,  Peter Harms

IPC分类号： G01N21/00 ,  G01N21/64 ,  G01N21/66 ,  G01N21/76 ,  G01N33/50 ,  G01N31/16 ,  G01N31/22 ,  G01J1/48

CPC分类号： G01N21/6428 ,  C09B57/001 ,  G01N31/221

摘要： The present invention provides ratiometric fluorescent pH sensors for non-invasive, continuous monitoring of pH in such applications as fermentation processes. The ratiometric fluorescent pH sensors comprise a fluorescent dye that exhibits a shift in excitation wavelength with a corresponding shift in pH in the local environment of said fluorescent dye. Ratiometric measurements of the emission intensities at dual excitation maxima correlate to pH. Also provided is a fluorescent dye 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS). Further provided are systems and methods to non-invasively and continuously monitor pH.

摘要翻译： 本发明提供了比例式荧光pH传感器，用于在诸如发酵过程的应用中非侵入性，连续监测pH。 比例荧光pH传感器包括荧光染料，其在所述荧光染料的局部环境中具有相应的pH偏移的激发波长的移动。 双重激发最大值的发射强度的比例测量与pH相关。 还提供了6-甲基丙烯酰基-8-羟基-1,3-芘二磺酸荧光染料（MA-HPDS）。 还提供了非侵入性和连续监测pH的系统和方法。