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    Ratiometric fluorescent pH sensor for non-invasive monitoring
    1.
    发明授权
    Ratiometric fluorescent pH sensor for non-invasive monitoring 有权
    比例式荧光pH传感器，用于非侵入性监测

    公开(公告)号：US07390462B2

    公开(公告)日：2008-06-24

    申请号：US10609720

    申请日：2003-06-30

    申请人： Govind Rao ,  Iordan V. Kostov ,  Haley R. Kermis ,  Peter Harms

    发明人： Govind Rao ,  Iordan V. Kostov ,  Haley R. Kermis ,  Peter Harms

    IPC分类号： G01N21/00 ,  G01N21/64 ,  G01N21/66 ,  G01N21/76 ,  G01N33/50 ,  G01N31/16 ,  G01N31/22 ,  G01J1/48

    CPC分类号： G01N21/6428 ,  C09B57/001 ,  G01N31/221

    摘要： The present invention provides ratiometric fluorescent pH sensors for non-invasive, continuous monitoring of pH in such applications as fermentation processes. The ratiometric fluorescent pH sensors comprise a fluorescent dye that exhibits a shift in excitation wavelength with a corresponding shift in pH in the local environment of said fluorescent dye. Ratiometric measurements of the emission intensities at dual excitation maxima correlate to pH. Also provided is a fluorescent dye 6-methacryloyl-8-hydroxy-1,3-pyrene disulfonic acid (MA-HPDS). Further provided are systems and methods to non-invasively and continuously monitor pH.

    摘要翻译： 本发明提供了比例式荧光pH传感器，用于在诸如发酵过程的应用中非侵入性，连续监测pH。 比例荧光pH传感器包括荧光染料，其在所述荧光染料的局部环境中具有相应的pH偏移的激发波长的移动。 双重激发最大值的发射强度的比例测量与pH相关。 还提供了6-甲基丙烯酰基-8-羟基-1,3-芘二磺酸荧光染料（MA-HPDS）。 还提供了非侵入性和连续监测pH的系统和方法。

    Device for discrimination of fluorescence lifetimes and uses therefor
    2.
    发明授权
    Device for discrimination of fluorescence lifetimes and uses therefor 失效

    公开(公告)号：US07041986B2

    公开(公告)日：2006-05-09

    申请号：US10389110

    申请日：2003-03-14

    申请人： Govind Rao ,  Peter Harms ,  Iordan V. Kostov

    发明人： Govind Rao ,  Peter Harms ,  Iordan V. Kostov

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6408 ,  G01N2021/6432

    摘要： The present invention provides methods for determining fluorescence lifetime of a fluorophor or an optical sensor using frequency domain gated detection. The method comprises the steps of exciting the fluorophor with frequency modulated light from a pulsed light source, gating a photodetector during excitation of the fluorophore, detecting light emitted from the fluorophor with the photodetector, where the emission light exhibits a phase shift in frequency from that of the excitation light, converting the detected excitation light to an amplified electric signal, and evaluating the amplified electric signal as a measure of fluorescence lifetime of the fluorophor. Also provided is a device with which to use the methods disclosed herein.