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1.发明申请METHODS AND SYSTEMS FOR REDUCING DNA FRAGMENTATION IN A POPULATION OF SPERM CELLS 审中-公开减少细胞中DNA分解的方法和系统公开(公告)号：US20120308998A1
公开(公告)日：2012-12-06
申请号：US13519114
申请日：2010-10-28
CPC分类号： C12N5/0612 , G01N21/6428 , G01N21/645
摘要： A method and system for sorting sperm samples according to different levels of DNA fragmentation and methods of using populations with low levels of DNA fragmentation to improve fertility and success rates of assisted reproductive procedures, including artificial insemination, in vitro fertilization, intracytoplasmic injection, and other related techniques.
摘要翻译： 根据不同DNA片段水平对精子样本进行分选的方法和系统，以及使用DNA片段低水平的群体的方法和方法，以改善辅助生殖程序（包括人工授精，体外受精，胞质内注射等）的生殖力和成功率 相关技术。
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公开(公告)号：US08492086B2
公开(公告)日：2013-07-23
申请号：US12513560
申请日：2007-11-08
申请人： Jaime Gosalvez Berenguer , José Luis Fernandez Garcia , Vicente Goyanes Villaescusa , German Bau Arevalo , Lourdes Muriel Rios , Monica Cartelle Gestal
发明人： Jaime Gosalvez Berenguer , José Luis Fernandez Garcia , Vicente Goyanes Villaescusa , German Bau Arevalo , Lourdes Muriel Rios , Monica Cartelle Gestal
IPC分类号： C07H21/00
CPC分类号： C12Q1/6841 , C12N15/1003
摘要： The invention relates to a method for determining DNA integrity in microorganisms and to a kit for evaluating DNA integrity in same. Since cell death results in DNA fragmentation, the inventive method can be used to determine DNA fragmentation levels in microorganisms clearly, simply, quickly and precisely.
摘要翻译： 本发明涉及一种确定微生物中DNA完整性的方法以及用于评估其完整性的试剂盒。 由于细胞死亡导致DNA片段化，本发明的方法可用于清楚地，简单地，快速和准确地测定微生物中的DNA断裂水平。
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公开(公告)号：US07993827B2
公开(公告)日：2011-08-09
申请号：US10586298
申请日：2005-01-26
CPC分类号： C12Q1/6827 , C12Q2527/137
摘要： The present invention describes a method for the determination of DNA fragmentation in animal sperm. It particularly refers to a procedure to evaluate the integrity of the chromatin/DNA of the sperm by means of a treatment of the sample with a denaturing solution followed, optionally by a stain; a subsequent treatment with a lysis solution that does not contain a protein denaturing detergent, followed, optionally, by a stain; and an evaluation of the integrity of the chromatin/DNA. The present invention also refers to a Kit to evaluate the quality of the sperm of animals which includes a DNA denaturing solution and a lysis solution that does not contain a protein denaturing detergent.
摘要翻译： 本发明描述了确定动物精子中DNA断裂的方法。 特别是指通过用变性溶液处理样品，随后通过染色来评估精子的染色质/ DNA的完整性的程序。 随后用不含蛋白质变性洗涤剂的裂解溶液处理，随后任选地通过染色剂处理; 并评估染色质/ DNA的完整性。 本发明还涉及用于评价动物精子质量的试剂盒，其包括DNA变性溶液和不含蛋白质变性洗涤剂的裂解液。
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公开(公告)号：US20140206573A1
公开(公告)日：2014-07-24
申请号：US14234875
申请日：2012-07-26
申请人： José Luis Fernandesz Garcia , Jaime Gosalvez Berenguer , German Bou Arevalo , María Tamaoyo Novas , Rebeca Santisto Brandariz
发明人： José Luis Fernandesz Garcia , Jaime Gosalvez Berenguer , German Bou Arevalo , María Tamaoyo Novas , Rebeca Santisto Brandariz
摘要： The present invention relates to a method for evaluating the integrity of the cell wall of the bacteria present in a culture in the presence of an antibiotic acting on the bacterial cell wall which, from a practical point of view, allows quickly determining if a bacterium is sensitive or resistant to an antibiotic acting on the bacterial cell wall. Likewise, the present invention also relates to a lysis solution applicable in the preceding method, specifically affecting bacteria having the cell wall damaged by the action of an antibiotic acting on the bacterial cell wall, which allows distinguishing bacteria sensitive to said antibiotic from those resistant to said antibiotic.
摘要翻译： 本发明涉及一种用于评估存在于作用于细菌细胞壁的抗生素存在下的培养物中存在的细菌的细胞壁的完整性的方法，从实践的角度来看，其允许快速确定细菌是否为 对抗细菌细胞壁作用的抗生素敏感或抗性。 同样地，本发明还涉及适用于上述方法的裂解液，特别是影响细胞壁受到作用于细菌细胞壁的抗生素作用而损伤细菌的细菌，其允许将对所述抗生素敏感的细菌与抗 说抗生素
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公开(公告)号：US20070281298A1
公开(公告)日：2007-12-06
申请号：US10586298
申请日：2005-01-26
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2527/137
摘要： The present invention describes a method for the determination of DNA fragmentation in animal sperm. It particularly refers to a procedure to evaluate the integrity of the chromatin/DNA of the sperm by means of a treatment of the sample with a denaturing solution followed, optionally by a stain; a subsequent treatment with a lysis solution that does not contain a protein denaturing detergent, followed, optionally, by a stain; and an evaluation of the integrity of the chromatin/DNA. The present invention also refers to a Kit to evaluate the quality of the sperm of animals which includes a DNA denaturing solution and a lysis solution that does not contain a protein denaturing detergent.
摘要翻译： 本发明描述了确定动物精子中DNA断裂的方法。 特别是指通过用变性溶液处理样品，随后通过染色来评估精子的染色质/ DNA的完整性的程序。 随后用不含蛋白质变性洗涤剂的裂解溶液处理，随后任选地通过染色剂处理; 并评估染色质/ DNA的完整性。 本发明还涉及用于评价动物精子质量的试剂盒，其包括DNA变性溶液和不含蛋白质变性洗涤剂的裂解液。
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公开(公告)号：US09976170B2
公开(公告)日：2018-05-22
申请号：US14234875
申请日：2012-07-26
申请人： Jose Luis Fernandez Garcia , Jaime Gosalvez Berenguer , German Bou Arevalo , Maria Tamayo Novas , Rebeca Santiso Brandariz
发明人： Jose Luis Fernandez Garcia , Jaime Gosalvez Berenguer , German Bou Arevalo , Maria Tamayo Novas , Rebeca Santiso Brandariz
摘要： The present invention relates to a method for evaluating the integrity of the cell wall of the bacteria present in a culture in the presence of an antibiotic acting on the bacterial cell wall which, from a practical point of view, allows quickly determining if a bacterium is sensitive or resistant to an antibiotic acting on the bacterial cell wall. Likewise, the present invention also relates to a lysis solution applicable in the preceding method, specifically affecting bacteria having the cell wall damaged by the action of an antibiotic acting on the bacterial cell wall, which allows distinguishing bacteria sensitive to said antibiotic from those resistant to said antibiotic.
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公开(公告)号：US20100129803A1
公开(公告)日：2010-05-27
申请号：US12513560
申请日：2007-11-08
申请人： Jaime Gosalvez Berenguer , José Luis Fernandez Garcia , Vicente Goyanes Villaescusa , German Bau Arevalo , Lourdes Muriel Rios , Monica Cartelle Gestal
发明人： Jaime Gosalvez Berenguer , José Luis Fernandez Garcia , Vicente Goyanes Villaescusa , German Bau Arevalo , Lourdes Muriel Rios , Monica Cartelle Gestal
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12N15/1003
摘要： The invention relates to a method for determining DNA integrity in microorganisms and to a kit for evaluating DNA integrity in same. Since cell death results in DNA fragmentation, the inventive method can be used to determine DNA fragmentation levels in microorganisms clearly, simply, quickly and precisely.
摘要翻译： 本发明涉及一种确定微生物中DNA完整性的方法以及用于评估其完整性的试剂盒。 由于细胞死亡导致DNA片段化，本发明的方法可用于清楚地，简单地，快速和准确地测定微生物中的DNA断裂水平。
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