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公开(公告)号：US07090979B2
公开(公告)日：2006-08-15
申请号：US10718856
申请日：2003-11-21
申请人： Raymond P. Mariella, Jr. , Allen T. Christian , James D. Tucker , John M. Dzenitis , Alexandros P. Papavasiliou
发明人： Raymond P. Mariella, Jr. , Allen T. Christian , James D. Tucker , John M. Dzenitis , Alexandros P. Papavasiliou
摘要： A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.
摘要翻译： 制备非常长的双链合成多核苷酸的方法。 提供多种短寡核苷酸。 短寡核苷酸相互杂交。 寡核苷酸的酶连接提供了预定序列的连续的PCR-准备DNA片段。
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公开(公告)号：US06783943B2
公开(公告)日：2004-08-31
申请号：US10032017
申请日：2001-12-18
IPC分类号： C12Q168
CPC分类号： C12Q1/6844 , C12Q1/6806 , C12Q2543/10 , C12Q2531/125 , C12Q2521/319 , C12Q2521/301
摘要： Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
摘要翻译： 滚环扩增（RCA）已被用于检测分离的核酸中的点突变，但其在细胞学制备中的应用是有问题的。 通过用限制酶和外切核酸酶的组合预处理细胞，我们在溶液中原位表达RCA，并原位检测基因拷贝数和单碱基突变。 它还可以检测和定量单个细胞中的转录RNA，使其成为基于细胞的测定的通用工具。
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公开(公告)号：US06432650B1
公开(公告)日：2002-08-13
申请号：US09705471
申请日：2000-11-02
IPC分类号： C12Q168
CPC分类号： C12Q1/6876 , C12Q2600/142 , C12Q2600/156 , C12Q2600/158
摘要： Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.
摘要翻译： 描述了原位扩增染色体DNA以增加染色体或染色体区域相关DNA的量。 原位染色体DNA的扩增提供了来自单个解剖的染色体片段的荧光原位杂交（FISH）绘画探针的合成，从低拷贝mRNA产生cDNA文库并在比较基因组杂交（CGH）方法中得到改进。
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