公开(公告)号：US20050221461A1

公开(公告)日：2005-10-06

申请号：US10500943

申请日：2003-01-16

申请人： Ayrookaran Poulose ,  David Estell ,  James Kellis Jr ,  Richard Bott

发明人： Ayrookaran Poulose ,  David Estell ,  James Kellis Jr ,  Richard Bott

IPC分类号： C12N15/09 ,  C07H21/04 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/50 ,  C12N9/52 ,  C12N9/54 ,  C12N9/56 ,  C12N15/57

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or re-combinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

摘要翻译： 公开了包含衍生自天然存在或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的另外的变体。

公开(公告)号：US20070134779A1

公开(公告)日：2007-06-14

申请号：US11583597

申请日：2006-10-18

申请人： Wade Dyson ,  James Kellis ,  Ayrookaran Poulose ,  Mee-Young Yoon

发明人： Wade Dyson ,  James Kellis ,  Ayrookaran Poulose ,  Mee-Young Yoon

IPC分类号： C12P7/62 ,  C08F20/00

CPC分类号： G06K9/6828

摘要： A method is provided for enzymatically modifying a polyester resin, film, fiber, yarn, fabric or textile to modify the characteristics thereof.

摘要翻译： 提供了对聚酯树脂，薄膜，纤维，纱线，织物或织物进行酶改性以改变其特性的方法。

公开(公告)号：US20060127278A1

公开(公告)日：2006-06-15

申请号：US10508294

申请日：2003-04-25

申请人： Alice Gast ,  James Kellis ,  Joon-H Kim ,  Ayrookaran Poulose ,  Shaunak Roy

发明人： Alice Gast ,  James Kellis ,  Joon-H Kim ,  Ayrookaran Poulose ,  Shaunak Roy

IPC分类号： G01N21/64

CPC分类号： G01N21/553 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  G01N21/648 ,  G01N2021/212

摘要： The present disclosure relates to a device for measuring surface plasmon resonance and fluorescence of a sample, a system for determining the rate of catalytic activity of an enzyme, a method of determining the rate of catalytic activity of an enzyme, and a method of measuring the adsorption and reactivity of a substance, all of which use SPR and SPEF methods simultaneously. This invention also relates generally to systems and methods for measuring diffusion and reactivity of macromolecules on a surface.

摘要翻译： 本公开涉及一种用于测量样品的表面等离子体共振和荧光的装置，用于确定酶的催化活性速率的系统，确定酶的催化活性速率的方法以及测量方法 物质的吸附和反应性，所有这些都同时使用SPR和SPEF方法。 本发明还涉及用于测量表面上大分子的扩散和反应性的系统和方法。