公开(公告)号：US20180312869A1

公开(公告)日：2018-11-01

申请号：US15766432

申请日：2016-10-06

申请人： INSTITUTE FOR BASIC SCIENCE ,  SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION ,  AICT

发明人： Jin Soo KIM ,  Jungeun KIM ,  Sunghwa CHOE ,  Je Wook WOO ,  Soon II KWON

IPC分类号： C12N15/82

CPC分类号： A01H5/12 ,  C12N15/8213 ,  C12N15/8298

摘要： The present invention relates to method for preparing a plant from a protoplast comprising knocking-out or knocking-in one or more the endogenous gene of the protoplast, and the plant regenerated from a genome-modified protoplast prepared by the above method.

公开(公告)号：US20130254934A1

公开(公告)日：2013-09-26

申请号：US13992485

申请日：2011-12-09

申请人： Takeshi Nakano ,  Hideo Nakashita ,  Tadao Asami ,  Michiko Yasuda ,  Ayumi Yamagami

发明人： Takeshi Nakano ,  Hideo Nakashita ,  Tadao Asami ,  Michiko Yasuda ,  Ayumi Yamagami

IPC分类号： C12N15/82

CPC分类号： C12N15/8281 ,  C07K14/415 ,  C12N15/8279 ,  C12N15/8298

摘要： It is intended to identify a novel gene involved in the induction of pathogen resistance by brassinosteroid and to prepare a disease-resistant plant with enhanced disease resistance by introducing the gene into plant cells of interest. Pathogen-resistant brassinosteroid mutants are isolated, and four genes obtained on the basis of the analysis of causative genes of the mutants were introduced into plant cells of interest to prepare transgenic plants.

摘要翻译： 旨在鉴定涉及通过油菜素类固醇诱导病原体抗性的新基因，并通过将基因导入目的植物细胞来制备具有增强的抗病性的抗病植物。 分离病原菌抗性油菜素固醇突变体，并将基于突变体致病基因分析获得的四个基因导入目的植物细胞中以制备转基因植物。

公开(公告)号：US20130007910A1

公开(公告)日：2013-01-03

申请号：US13473426

申请日：2012-05-16

申请人： Michael Hothorn ,  Joanne Chory

发明人： Michael Hothorn ,  Joanne Chory

IPC分类号： C12N9/12 ,  G06F19/16 ,  G01N33/566

CPC分类号： C07K14/415 ,  C12N15/8261 ,  C12N15/8298 ,  Y02A40/146

摘要： Provided herein is the crystal structure for the brassinosteroid receptor BRI1, as well as strategies for modulating its activity.

摘要翻译： 本文提供了油菜素甾体受体BRI1的晶体结构，以及调节其活性的策略。

公开(公告)号：US07132524B2

公开(公告)日：2006-11-07

申请号：US10303996

申请日：2002-11-26

申请人： Setsuko Komatsu ,  Guangxiao Yang

发明人： Setsuko Komatsu ,  Guangxiao Yang

IPC分类号： C12N15/29

CPC分类号： C07K14/415 ,  C12N15/8298

摘要： The objective of the present invention is to isolate and identify genes responsive to plant hormones, such as, brassinolide, and to provide these genes and their use. The present inventors found two novel genes (referred to as OsBLE1 gene and OsBLE2 gene) whose expressions are markedly increased by brassinolide and auxin, using DNA microarray techniques and Northern blotting. Transformed rice plants were produced using Agrobacterium EHA101 which comprise antisense polynucleotide against OsBLE1 and OsBLE2 under the control of the CaMV35S promoter in a binary vector pIG121-Hm. As a result, the transformed rice plants exhibited inhibition in stem and leaf growth as compared with controls which carried the vector alone.

公开(公告)号：US5907082A

公开(公告)日：1999-05-25

申请号：US560398

申请日：1995-11-17

申请人： Sharman O'Neill ,  Jeanette Nadeau

发明人： Sharman O'Neill ,  Jeanette Nadeau

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00 ,  C07H21/04

CPC分类号： C07K14/415 ,  C12N15/8233 ,  C12N15/8287 ,  C12N15/8297 ,  C12N15/8298

摘要： The present invention provides nucleic acid sequences from ovule-specific genes. The nucleic acids are useful in targeting gene expression to ovules or in modulating ovule development.

摘要翻译： 本发明提供了来自胚珠特异性基因的核酸序列。 核酸可用于靶向胚珠的基因表达或调节胚珠发育。

公开(公告)号：US09512440B2

公开(公告)日：2016-12-06

申请号：US13773150

申请日：2013-02-21

申请人： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

发明人： Hongqing Guo ,  Yanhai Yin

IPC分类号： C12N15/87 ,  C12N15/82

CPC分类号： C12N15/8261 ,  C12N15/8281 ,  C12N15/8298 ,  Y02A40/146

摘要： The invention provides the disclosure of a novel plant growth pathway involving several receptor like kinases. The pathway was shown to work largely independent of brassinosteroids and involves several members of the CrRLK1L family of receptor kinases. According to the invention, HERK1, HERK2, FER, THE1 and the brassinosteroid BES1 may be modulated to influence plant growth and elongation. The invention includes methods, and transformed plants, cells tissues and seeds with increased cellular elongation and other related yield traits.

摘要翻译： 本发明提供涉及几种受体类激酶的新型植物生长途径的公开。 该途径被证明在很大程度上独立于油菜素类固醇，并且涉及CrRLK1L家族受体激酶的几个成员。 根据本发明，可以调节HERK1，HERK2，FER，THE1和油菜素甾体BES1以影响植物的生长和伸长。 本发明包括方法和转化的植物，细胞组织和具有增加的细胞延伸和其他相关产量性状的种子。

公开(公告)号：US08461413B2

公开(公告)日：2013-06-11

申请号：US12524746

申请日：2008-01-30

申请人： Valerie Frankard

发明人： Valerie Frankard

IPC分类号： A01H1/00 ,  A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/63 ,  C12N15/29

CPC分类号： C12N15/8298

摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for increasing seed yield in plants by increasing expression in a plant of a nucleic acid sequence encoding a Dwarf1 (DWF1) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a DWF1 polypeptide, which plants have increased seed yield relative to control plants. The invention also provides constructs useful in performing the methods of the invention.

摘要翻译： 本发明一般涉及分子生物学领域，涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地，本发明涉及通过增加植物中编码Dwarf1（DWF1）多肽的核酸序列的表达来提高植物中种子产量的方法。 本发明还涉及具有增加的编码DWF1多肽的核酸序列表达的植物，该植物相对于对照植物具有增加的种子产量。 本发明还提供了用于实施本发明方法的构建体。

公开(公告)号：US08008542B2

公开(公告)日：2011-08-30

申请号：US12008398

申请日：2008-01-09

申请人： Xuelu Wang ,  Joanne Chory

发明人： Xuelu Wang ,  Joanne Chory

IPC分类号： A01H1/00 ,  A01H5/00

CPC分类号： C12N15/8261 ,  C07K14/415 ,  C12N15/8298 ,  Y02A40/146

摘要： This invention provides recombinant cells and transgenic plants that display selectively increased or decreased response to brassinosteroids, resulting in increased yield. Methods of modulating brassinosteroid responses, and of modulating plant phenotypes, are provided.

摘要翻译： 本发明提供重组细胞和转基因植物，其显示选择性增加或减少对油菜素固醇的反应，导致产量增加。 提供了调节油菜素类固醇反应和调节植物表型的方法。

公开(公告)号：US20110072537A1

公开(公告)日：2011-03-24

申请号：US11111760

申请日：2005-04-22

申请人： Ousama M. Zaghmout

发明人： Ousama M. Zaghmout

IPC分类号： C12N15/11 ,  A01H5/00

CPC分类号： C12N15/8298 ,  C12N15/8273 ,  C12N15/8286 ,  Y02A40/162

摘要： A method for genetically modifying a turfgrass plant cell such that a plant, produced from said cell, is characterized as having modulated brassinolide activity as compared with a wild-type plant, said method comprising: introducing at least one exogenous BAS1 polynucleotide, homologue or functional fragment thereof, into a plant cell to obtain a transformed plant cell; and growing the transformed plant cell under conditions which permit expression of BAS1 gene product, homologue or functional fragment thereof, thereby producing a plant having modulated brassinolide activity, wherein the plant cell is a turf grass cell wherein said genetically modified plant further comprising a drought resistance, salt resistance, insect resistance and the like.

摘要翻译： 一种用于遗传修饰草坪草植物细胞的方法，使得从所述细胞产生的植物的特征在于与野生型植物相比具有调制的油菜素内酯活性，所述方法包括：引入至少一种外源性的BAS1多核苷酸，同系物或功能性 其片段进入植物细胞以获得转化的植物细胞; 以及在允许表达BAS1基因产物，同源物或其功能片段的条件下生长转化的植物细胞，从而产生具有调制油菜素内酯活性的植物，其中所述植物细胞是草坪草细胞，其中所述转基因植物还包含抗旱性 ，耐盐性，抗虫性等。

公开(公告)号：US20100011464A1

公开(公告)日：2010-01-14

申请号：US12524746

申请日：2008-01-30

申请人： Valerie Frankard

发明人： Valerie Frankard

IPC分类号： A01H1/00 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8298

摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for increasing seed yield in plants by increasing expression in a plant of a nucleic acid sequence encoding a Dwarf1 (DWF1) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding a DWF1 polypeptide, which plants have increased seed yield relative to control plants. The invention also provides constructs useful in performing the methods of the invention.

摘要翻译： 本发明一般涉及分子生物学领域，涉及增强植物中各种经济上重要的产量相关性状的方法。 更具体地，本发明涉及通过增加植物中编码Dwarf1（DWF1）多肽的核酸序列的表达来提高植物中种子产量的方法。 本发明还涉及具有增加的编码DWF1多肽的核酸序列表达的植物，该植物相对于对照植物具有增加的种子产量。 本发明还提供了用于实施本发明方法的构建体。