公开(公告)号：US5670706A

公开(公告)日：1997-09-23

申请号：US47413

申请日：1993-04-19

申请人： Bernardus J. C. Cornelissen ,  Leo Sjoerd Melchers ,  Elisabeth J. S. Meulenhoff ,  Jeroen S. C. van Roekel ,  Marianne Beatrix Sela-Buurlage ,  Alexandra Aleida Vloemans ,  Charles Peter Woloshuk ,  John Ferdinand Bol ,  Hubertus J. M. Linthorst

发明人： Bernardus J. C. Cornelissen ,  Leo Sjoerd Melchers ,  Elisabeth J. S. Meulenhoff ,  Jeroen S. C. van Roekel ,  Marianne Beatrix Sela-Buurlage ,  Alexandra Aleida Vloemans ,  Charles Peter Woloshuk ,  John Ferdinand Bol ,  Hubertus J. M. Linthorst

IPC分类号： A01H5/00 ,  A01H1/00 ,  A01N65/00 ,  C12N20060101 ,  C12N1/20 ,  C12N1/21 ,  C12N9/24 ,  C12N15/00 ,  C12N15/09 ,  C12N15/29 ,  C12N15/52 ,  C12N15/56 ,  C12N15/63 ,  C12N15/82 ,  A01H4/00

CPC分类号： C12N15/8282 ,  A01N65/38 ,  C12N9/244 ,  C12N9/2442 ,  C12Y302/01006 ,  C12Y302/01014 ,  C12Y302/01039 ,  C12Y302/01058

摘要： Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and .beta.-1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a .beta.-1,3-glucanase.

摘要翻译： 植物具有改善的抗病原菌的抗性。 它们用一种或多种基本上包含编码植物几丁质酶和β-1,3-葡聚糖酶的一种或多种基因的多核苷酸遗传转化。 优选的是所述水解酶的细胞内形式，特别优选的是通过修饰编码所述酶的基因而靶向植物的外胚层空间的那些形式。 特别优选的是表现出编码几丁质酶的至少一种基因和编码β-1,3-葡聚糖酶的一种基因的相对过表达的植物。

公开(公告)号：US6087560A

公开(公告)日：2000-07-11

申请号：US801563

申请日：1997-02-18

申请人： Bernardus J. C. Cornelissen ,  Leo Sjoerd Melchers ,  Elisabeth J. S. Meulenhoff ,  Jeroen S. C. van Roekel ,  Marianne Beatrix Sela-Buurlage ,  Alexandra Aleida Vloemans ,  Charles Peter Woloshuk ,  John Ferdinand Bol ,  Hubertus J. M. Linthorst

发明人： Bernardus J. C. Cornelissen ,  Leo Sjoerd Melchers ,  Elisabeth J. S. Meulenhoff ,  Jeroen S. C. van Roekel ,  Marianne Beatrix Sela-Buurlage ,  Alexandra Aleida Vloemans ,  Charles Peter Woloshuk ,  John Ferdinand Bol ,  Hubertus J. M. Linthorst

IPC分类号： C12N9/24 ,  C12N15/82 ,  A01H5/00 ,  C12N1/21

CPC分类号： C12Y302/01039 ,  A01N65/38 ,  C12N15/8282 ,  C12N9/2405 ,  C12N9/2442 ,  C12Y302/01014

摘要： Plants are provided with improved resistance against pathogenic fungi. They are genetically transformed with one or more polynucleotides which essentially comprise one or more genes encoding plant chitinases and .beta.-1,3-glucanases. Preferred are the intracellular forms of the said hydrolytic enzymes, especially preferred are those forms which are targeted to the apoplastic space of the plant by virtue of the modification of the genes encoding the said enzymes. Particularly preferred are plants exhibiting a relative overexpression of at least one gene encoding a chitinase and one gene encoding a .beta.-1,3-glucanase.

摘要翻译： 植物具有改善的抗病原菌的抗性。 它们用一种或多种基本上包含编码植物几丁质酶和β-1,3-葡聚糖酶的一种或多种基因的多核苷酸遗传转化。 优选的是所述水解酶的细胞内形式，特别优选的是通过修饰编码所述酶的基因而靶向植物的外胚层空间的那些形式。 特别优选的是表现出编码几丁质酶的至少一种基因和编码β-1,3-葡聚糖酶的一种基因的相对过表达的植物。