公开(公告)号：US20080090250A1

公开(公告)日：2008-04-17

申请号：US11703404

申请日：2007-02-06

Applicant: Jianfu Wang ,  Weixing Hu

Inventor： Jianfu Wang ,  Weixing Hu

IPC: G01N33/53

CPC classification number: C40B30/04 ,  C07K16/00 ,  C07K16/1217 ,  C07K16/40

Abstract: The present invention relates generally to sets of digital antibodies directed against short epitopes, and use thereof in methods for protein analysis.

Abstract translation: 本发明一般涉及针对短表位的数字抗体集，以及其在蛋白质分析方法中的应用。

公开(公告)号：US20070196867A1

公开(公告)日：2007-08-23

申请号：US11529826

申请日：2006-09-29

Applicant: Helena Mancebo ,  Jeng-Horng Her ,  Samuel Li ,  Jianfu Wang

Inventor： Helena Mancebo ,  Jeng-Horng Her ,  Samuel Li ,  Jianfu Wang

IPC: G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C12P21/04 ,  C07K14/705 ,  C07K16/28 ,  C12N15/86

CPC classification number: G01N33/68 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/6845 ,  G01N33/6851 ,  G01N2333/726 ,  G01N2500/00 ,  G01N2800/52

Abstract: The present invention provides expression vectors that facilitate high levels of expression of GPCR proteins. Encompassed by the invention are methods and compositions for recombinant cell lines expressing GPCR proteins with the aid of the expression vectors of the instant invention. The recombinant cell lines of the instant invention express GPCR proteins at levels of at least about 150,000 copies of the protein per cell. The present invention also provides methods and compositions for raising antibodies against GPCR proteins using the high expressing recombinant cells of the instant invention.

Abstract translation: 本发明提供促进GPCR蛋白表达水平高的表达载体。 本发明所涵盖的是借助于本发明的表达载体表达GPCR蛋白的重组细胞系的方法和组合物。 本发明的重组细胞系以每个细胞至少约15万拷贝的蛋白质的量表达GPCR蛋白质。 本发明还提供使用本发明的高表达重组细胞提高针对GPCR蛋白的抗体的方法和组合物。