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公开(公告)号：US20050089950A1
公开(公告)日：2005-04-28
申请号：US10504258
申请日：2003-02-20
CPC分类号： C12N15/1003
摘要： The present invention relates to the coarse clarification of lysed cell material from microorganisms and particularly to a method of obtaining nucleic acids.
摘要翻译： 本发明涉及从微生物中裂解的细胞材料的粗略澄清，特别涉及获得核酸的方法。
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公开(公告)号：US07214508B2
公开(公告)日：2007-05-08
申请号：US10504258
申请日：2003-02-20
CPC分类号： C12N15/1003
摘要： The present invention relates to the coarse clarification of lysed cell material from microorganisms and particularly to a method of obtaining nucleic acids. In certain embodiments, the present invention provides a method of coarsely clarifying cell lysate from microorganisms that comprises: lysing the microorganisms under the normal atmospheric pressure to form a flocculent precipitate in the lysate, compressing the flocculent precipitate in the lysate by reducing or increasing the pressure of the atmosphere surrounding the lysate in relation to the normal atmospheric pressure to form a compressed flocculent phase and a liquid phase, and separating the two phases.
摘要翻译： 本发明涉及从微生物中裂解的细胞材料的粗略澄清，特别涉及获得核酸的方法。 在某些实施方案中，本发明提供了从微生物粗略澄清细胞裂解物的方法，其包括：在正常大气压下裂解微生物以在裂解物中形成絮状沉淀物，通过降低或增加压力来压缩裂解物中的絮状沉淀物 的相对于正常大气压的裂解物周围的气氛，形成压缩的絮凝相和液相，并分离两相。
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公开(公告)号：US09458494B2
公开(公告)日：2016-10-04
申请号：US13703205
申请日：2011-06-14
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： Provided is a method for extracting nucleic acids from a wax-embedded sample, and use of particular solvents for removing wax from a wax-embedded sample for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample.
摘要翻译： 本发明提供从蜡嵌入样品中提取核酸的方法，使用特定溶剂从蜡嵌入样品中除去蜡，从交联的蜡嵌入样品中提取，分离和/或纯化核酸。
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公开(公告)号：US20130280787A1
公开(公告)日：2013-10-24
申请号：US13703205
申请日：2011-06-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1003
摘要： The present invention relates to a method for extracting nucleic acids from a wax-embedded sample, the use of particular solvents for removing wax from a wax-embedded sample in a method for extracting, isolating and/or purifying nucleic acids from a crosslinked wax-embedded sample as well as to a kit for extracting, isolating and/or purifying nucleic acids from a wax-embedded sample.
摘要翻译： 本发明涉及一种从嵌入蜡的样品中提取核酸的方法，在从交联的蜡 - 样品中提取，分离和/或纯化核酸的方法中使用特定溶剂从蜡包埋的样品中除去蜡， 嵌入样品以及用于从蜡嵌入样品中提取，分离和/或纯化核酸的试剂盒。
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