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    Method for bisulfite treatment
    7.
    发明申请
    Method for bisulfite treatment 有权
    亚硫酸氢盐处理方法

    公开(公告)号：US20060058518A1

    公开(公告)日：2006-03-16

    申请号：US10540406

    申请日：2004-01-28

    申请人： Frank Bergmann ,  Christine Market-Hahn ,  Joerg Kleiber ,  Dirk Block

    发明人： Frank Bergmann ,  Christine Market-Hahn ,  Joerg Kleiber ,  Dirk Block

    IPC分类号： C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C01D5/145 ,  C12Q1/6827 ,  C12Q2527/101 ,  C12Q2523/125

    摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.

    摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。