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    Method for bisulfite treatment
    1.
    发明申请
    Method for bisulfite treatment 有权
    亚硫酸氢盐处理方法

    公开(公告)号：US20060058518A1

    公开(公告)日：2006-03-16

    申请号：US10540406

    申请日：2004-01-28

    申请人： Frank Bergmann ,  Christine Market-Hahn ,  Joerg Kleiber ,  Dirk Block

    发明人： Frank Bergmann ,  Christine Market-Hahn ,  Joerg Kleiber ,  Dirk Block

    IPC分类号： C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C01D5/145 ,  C12Q1/6827 ,  C12Q2527/101 ,  C12Q2523/125

    摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.

    摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。

    Method for bisulfite treatment
    2.
    发明授权
    Method for bisulfite treatment 有权
    亚硫酸氢盐处理方法

    公开(公告)号：US07413855B2

    公开(公告)日：2008-08-19

    申请号：US10540406

    申请日：2004-01-28

    申请人： Frank Bergmann ,  Christine Markert-Hahn ,  Joerg Kleibler ,  Dirk Block

    发明人： Frank Bergmann ,  Christine Markert-Hahn ,  Joerg Kleibler ,  Dirk Block

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C07H21/04 ,  C01D5/145 ,  C12Q1/6827 ,  C12Q2527/101 ,  C12Q2523/125

    摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.

    摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。

    Dna decontamination method
    4.
    发明申请
    Dna decontamination method 有权
    Dna去污方法

    公开(公告)号：US20090208930A1

    公开(公告)日：2009-08-20

    申请号：US11658569

    申请日：2005-09-02

    申请人： Frank Bergmann ,  Christine Markert-Hahn

    发明人： Frank Bergmann ,  Christine Markert-Hahn

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6848 ,  C12Q1/6858 ,  C12Q2523/125 ,  C12Q2521/331

    摘要： The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.

    摘要翻译： 本发明涉及一种用于扩增样品中的甲基化靶核酸的方法，同时避免通过使其失活来扩增非甲基化靶核酸。 这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。 本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。

    Process for detecting nucleic acids by mass determination
    5.
    发明授权
    Process for detecting nucleic acids by mass determination 有权
    通过质量测定检测核酸的方法

    公开(公告)号：US06235476B1

    公开(公告)日：2001-05-22

    申请号：US09242536

    申请日：1999-03-17

    申请人： Frank Bergmann ,  Rupert Herrmann ,  Uwe Kobold

    发明人： Frank Bergmann ,  Rupert Herrmann ,  Uwe Kobold

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6872 ,  C12Q1/6827 ,  Y10T436/143333

    摘要： A method for detecting nucleic acids by binding a probe P to a partial sequence S contained in the nucleic acid to produce a binding product B1, degrading the nucleic acid to produce a binding product B2 containing a partial nucleic acid F of a defined length and detecting the binding product B2 or the partial nucleic acid F a part based on its mass is particularly suitable for the parallel detection of nucleic acid of different sequences.

    摘要翻译： 通过将探针P与核酸中所含的部分序列S结合以产生结合产物B1来检测核酸的方法，使核酸降解以产生含有限定长度的部分核酸F的结合产物B2并检测 结合产物B2或部分核酸F基于其质量的部分特别适用于不同序列的核酸的平行检测。

    Use of TDE for isolation of nucleic acids
    9.
    发明授权
    Use of TDE for isolation of nucleic acids 有权
    使用TDE分离核酸

    公开(公告)号：US08124338B2

    公开(公告)日：2012-02-28

    申请号：US12481807

    申请日：2009-06-10

    申请人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    发明人： Horst Donner ,  Frank Bergmann ,  Nina Lassonczyk ,  Manfred Watzele ,  Marcus Schmid

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12N15/1006

    摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

    摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。

    METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR
    10.
    发明申请
    METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR 审中-公开
    用于检测RNA分子的方法，与其相关的试剂盒及其用途

    公开(公告)号：US20110151444A1

    公开(公告)日：2011-06-23

    申请号：US12968322

    申请日：2010-12-15

    申请人： Andreas Albers ,  Waltraud Ankenbauer ,  Frank Bergmann ,  Stefanie Froehner ,  Dieter Heindl

    发明人： Andreas Albers ,  Waltraud Ankenbauer ,  Frank Bergmann ,  Stefanie Froehner ,  Dieter Heindl

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2525/207 ,  C12Q2525/186 ,  C12Q2525/155

    摘要： Described is a method for the detection of a RNA molecule, the method involving the steps of providing a sample containing the RNA molecule; hybridizing to the RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. Also described is a kit containing a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety.

    摘要翻译： 描述了用于检测RNA分子的方法，该方法包括提供含有RNA分子的样品的步骤; 与RNA分子杂交第一多核苷酸; 扩展第一多核苷酸以产生第一链cDNA; 将第二多核苷酸与第一链cDNA杂交; 延伸第一链cDNA以产生延伸反应产物; 通过聚合酶链反应扩增延伸反应产物; 并通过实时荧光读数检测扩增产物。 还描述了包含本发明中定义的第一和第二多核苷酸的试剂盒，一组dNTP，逆转录酶和检测部分。