公开(公告)号：US07425309B2

公开(公告)日：2008-09-16

申请号：US10602981

申请日：2003-06-24

Applicant: John D. Roback ,  Christopher D. Hillyer

Inventor： John D. Roback ,  Christopher D. Hillyer

IPC: G01N33/00

CPC classification number: G01N35/028 ,  B01L3/5025 ,  B01L3/50255 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0851 ,  G01N2035/00356 ,  G01N2035/00485 ,  G01N2035/0097

Abstract: An immunological or immunohematological assay system is disclosed that includes a vessel capable of containing an assay sample, an incubator, a sample separation system, an image acquisition system, and a pipettor. The immunological assay system may also include a washer. Also disclosed is an immunological assay method that includes the steps of placing a immunological assay sample in a vessel, which may include a filter; adding testing reagents to the vessel; incubating the sample and reagent mixture in the vessel; separating the sample and reagent mixture in the vessel into components that have and have not reacted; and analyzing the vessel to determine the presence of interactions between the sample and reagents. The bottom of the vessel is preferably of a material that aids in spreading out the reacted components of the sample evenly over the bottom of the vessel so that interactions can more easily be analyzed.

Abstract translation: 公开了免疫或免疫血液学测定系统，其包括能够包含测定样品，培养箱，样品分离系统，图像采集系统和移液器的容器。 免疫测定系统还可以包括洗衣机。 还公开了一种免疫测定方法，其包括将免疫测定样品置于容器中的步骤，容器可以包括过滤器; 向该容器加入测试试剂; 将样品和试剂混合物孵育在容器中; 将容器中的样品和试剂混合物分离成具有且没有反应的组分; 并分析血管以确定样品和试剂之间的相互作用的存在。 容器的底部优选是有助于将样品的反应组分均匀地扩散到容器底部的材料，使得可以更容易地分析相互作用。

公开(公告)号：US08486408B2

公开(公告)日：2013-07-16

申请号：US13121016

申请日：2009-10-02

Applicant: Mohammad S. Hossain ,  Andrew T. Gewitz ,  John D. Roback ,  Edmund K. Waller

Inventor： Mohammad S. Hossain ,  Andrew T. Gewitz ,  John D. Roback ,  Edmund K. Waller

IPC: A61K35/28 ,  A61K39/085

CPC classification number: A61K38/164 ,  A61K35/28 ,  A61K39/00 ,  A61K39/001 ,  A61K39/085 ,  Y02A50/466 ,  A61K2300/00

Abstract: Methods are disclosed for treating or preventing graft versus host disease in a subject. The methods include selecting a subject in need of treatment for graft versus host disease; and administering to the subject a therapeutically effective amount of a TLR5 agonist such as a flagellin polypeptide, or a polynucleotide encoding the flagellin, thereby treating or preventing graft versus host disease in the subject. Methods are also disclosed for reducing susceptibility to an opportunistic infection in a subject who is a bone marrow transplant recipient. The methods include selecting a subject who has had a bone marrow or hematopoietic stem cell transplant; and administering to the subject a therapeutically effective amount of a TLR5 agonist such as a flagellin polypeptide or a polynucleotide encoding the polypeptide, and administering to the subject an effective amount antigen of the opportunistic infection, thereby reducing the susceptibility to the opportunistic infection in the subject.

Abstract translation: 公开了用于治疗或预防受试者中移植物抗宿主病的方法。 所述方法包括选择需要治疗的受试者与移植物抗宿主病; 并向受试者施用治疗有效量的TLR5激动剂如鞭毛蛋白多肽或编码鞭毛蛋白的多核苷酸，从而治疗或预防受试者中的移植物抗宿主病。 还公开了减少作为骨髓移植受体的受试者的机会性感染的易感性的方法。 所述方法包括选择具有骨髓或造血干细胞移植的受试者; 并向受试者施用治疗有效量的TLR5激动剂如鞭毛蛋白多肽或编码该多肽的多核苷酸，并向受试者施用有效量的机会感染抗原，从而降低对受试者机会性感染的易感性 。

公开(公告)号：US20110206699A1

公开(公告)日：2011-08-25

申请号：US13121016

申请日：2009-10-02

Applicant: Mohammad S. Hossain ,  Andrew T. Gewitz ,  John D. Roback ,  Edmund K. Waller

Inventor： Mohammad S. Hossain ,  Andrew T. Gewitz ,  John D. Roback ,  Edmund K. Waller

IPC: A61K39/395 ,  A61K39/02 ,  A61K39/112 ,  A61P37/06

CPC classification number: A61K38/164 ,  A61K35/28 ,  A61K39/00 ,  A61K39/001 ,  A61K39/085 ,  Y02A50/466 ,  A61K2300/00

Abstract: Methods are disclosed for treating or preventing graft versus host disease in a subject. The methods include selecting a subject in need of treatment for graft versus host disease; and administering to the subject a therapeutically effective amount of a TLR5 agonist such as a flagellin polypeptide, or a polynucleotide encoding the flagellin, thereby treating or preventing graft versus host disease in the subject. Methods are also disclosed for reducing susceptibility to an opportunistic infection in a subject who is a bone marrow transplant recipient. The methods include selecting a subject who has had a bone marrow or hematopoietic stem cell transplant; and administering to the subject a therapeutically effective amount of a TLR5 agonist such as a flagellin polypeptide or a polynucleotide encoding the polypeptide, and administering to the subject an effective amount antigen of the opportunistic infection, thereby reducing the susceptibility to the opportunistic infection in the subject.

Abstract translation: 公开了用于治疗或预防受试者中移植物抗宿主病的方法。 所述方法包括选择需要治疗的受试者与移植物抗宿主病; 并向受试者施用治疗有效量的TLR5激动剂如鞭毛蛋白多肽或编码鞭毛蛋白的多核苷酸，从而治疗或预防受试者中的移植物抗宿主病。 还公开了减少作为骨髓移植受体的受试者的机会性感染的易感性的方法。 所述方法包括选择具有骨髓或造血干细胞移植的受试者; 并向受试者施用治疗有效量的TLR5激动剂如鞭毛蛋白多肽或编码该多肽的多核苷酸，并向受试者施用有效量的机会感染抗原，从而降低对受试者机会性感染的易感性 。

公开(公告)号：US07713708B2

公开(公告)日：2010-05-11

申请号：US11679589

申请日：2007-02-27

Applicant: John D. Roback ,  Christopher D. Hillyer

Inventor： John D. Roback ,  Christopher D. Hillyer

IPC: G01N33/53

CPC classification number: B01L3/50255 ,  B01L3/5025 ,  B01L2300/0829 ,  B01L2300/0851 ,  G01N35/028 ,  G01N2035/00356 ,  G01N2035/00485 ,  G01N2035/0097 ,  Y10T436/11 ,  Y10T436/111666

Abstract: An immunological or immunohematological assay system is disclosed that includes a filter vessel capable of containing an assay sample, an incubator, a sample separation system, an image acquisition system, and a robotic pipettor. The immunological assay system may also include a washer. Also disclosed is an immunological assay method that includes the steps of placing a immunological assay sample in a filter vessel, which includes a filter, adding testing reagents to the filter vessel, incubating the sample and reagent mixture in the filter vessel, separating the sample and reagent mixture in the filter vessel into components above and below the filter, and analyzing the filter vessel to determine the presence of interactions between the sample and reagents.

Abstract translation: 公开了免疫学或免疫血液学测定系统，其包括能够包含测定样品，培养箱，样品分离系统，图像采集系统和机器人移液器的过滤器容器。 免疫测定系统还可以包括洗衣机。 还公开了一种免疫测定方法，其包括以下步骤：将免疫测定样品置于过滤容器中，所述过滤容器包括过滤器，向过滤器容器中加入测试试剂，将样品和试剂混合物孵育在过滤容器中，分离样品和 将过滤容器中的试剂混合物转化成过滤器上方和下方的组分，并分析过滤器容器以确定样品与试剂之间的相互作用的存在。

公开(公告)号：US07189357B2

公开(公告)日：2007-03-13

申请号：US09773826

申请日：2001-01-31

Applicant: John D. Roback ,  Christopher D. Hillyer

Inventor： John D. Roback ,  Christopher D. Hillyer

IPC: G01N35/00

CPC classification number: B01L3/50255 ,  B01L3/5025 ,  B01L2300/0829 ,  B01L2300/0851 ,  G01N35/028 ,  G01N2035/00356 ,  G01N2035/00485 ,  G01N2035/0097 ,  Y10T436/11 ,  Y10T436/111666

Abstract: An immunological or immunohematological assay system is disclosed that includes a filter vessel capable of containing an assay sample, an incubator, a sample separation system, an image acquisition system, and a robotic pipettor. The immunological assay system may also include a washer. Also disclosed is an immunological assay method that includes the steps of placing a immunological assay sample in a filter vessel, which includes a filter, adding testing reagents to the filter vessel, incubating the sample and reagent mixture in the filter vessel, separating the sample and reagent mixture in the filter vessel into components above and below the filter, and analyzing the filter vessel to determine the presence of interactions between the sample and reagents.

Abstract translation: 公开了免疫学或免疫血液学测定系统，其包括能够包含测定样品，培养箱，样品分离系统，图像采集系统和机器人移液器的过滤器容器。 免疫测定系统还可以包括洗衣机。 还公开了一种免疫测定方法，其包括以下步骤：将免疫测定样品置于过滤容器中，所述过滤容器包括过滤器，向过滤器容器中加入测试试剂，将样品和试剂混合物孵育在过滤容器中，分离样品和 将过滤容器中的试剂混合物转化成过滤器上方和下方的组分，并分析过滤器容器以确定样品与试剂之间的相互作用的存在。