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公开(公告)号：US5221608A
公开(公告)日：1993-06-22
申请号：US428494
申请日：1989-10-26
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , C12Q1/6876 , C12Q1/703 , Y10S435/808 , Y10S436/805
摘要： Method for treating nucleic acid that is to be amplified such that the nucleic acid is, after amplification, subsequently unamplifiable. Nucleic acid amplification is performed in a reaction containing means in the presence of an isopsoralen. The reaction is thereafter irradiated to render the amplified nucleic acid substantially unamplifiable.
摘要翻译： 用于处理待扩增的核酸使得核酸在扩增后随后不可扩增的方法。 在含有补骨脂素的装置中进行核酸扩增。 然后将反应物照射以使扩增的核酸基本上不可扩增。
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公开(公告)号：US5139940A
公开(公告)日：1992-08-18
申请号：US427303
申请日：1989-10-26
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , Y10S435/81
摘要： The invention is directed to isopsoralen compound compositions that contain a variety of substitutions which yet permit their binding to nucleic acid polymers. Reaction conditions that activate these bound isopsoralens results in covalent crosslinking to the nucleic acid polymers. The resultant complex is inhibited from being a template for the template-directed enzymatic synthesis of a complementary nucleic acid polymer. Beside the isopsoralens compositions, the invention includes their synthesis, the nucleic acid complex production and detection, and the above inhibitory method of use of the isopsoralens.
摘要翻译： 本发明涉及含有多种替代物的异补骨脂素化合物组合物，其可以与核酸聚合物结合。 激活这些结合的异补骨脂素的反应条件导致与核酸聚合物的共价交联。 所得复合物被抑制为用于互补核酸聚合物的模板定向酶合成的模板。 除了异消消旋体组合物之外，本发明包括它们的合成，核酸复合体的产生和检测以及上述异丙屈肌酸酯的抑制方法。
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公开(公告)号：US5532146A
公开(公告)日：1996-07-02
申请号：US263020
申请日：1994-06-20
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , Y10S435/81
摘要： Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
摘要翻译： 连接酶扩增产物的灭菌方法，包括连接扩增反应（LAR）的产物，涉及在扩增后处理核酸，使得这种核酸不能作为进一步扩增的模板。 杀菌化合物，特别是光活化化合物的随机和位点特异性添加使随后的扩增产物不可扩增。 还使用自消毒引物，允许基于连接酶的扩增产物的均匀检测。
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公开(公告)号：US5532145A
公开(公告)日：1996-07-02
申请号：US979789
申请日：1992-11-20
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70 , C07H21/04
CPC分类号： C12Q1/6848 , B01L7/00 , B01L7/52 , C07D311/18 , C07D493/04 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6876 , C12Q1/703 , Y10S435/81
摘要： Method useful for solving the problem of contamination of protein with nucleic acid. Rendering amplifiable contaminating nucleic acid in enzyme preparations substantially unamplifiable prior to use of the enzyme.
摘要翻译： 用于解决核酸污染蛋白质问题的方法。 在使用酶之前，在酶制剂中呈现可扩增的污染性核酸基本上是不可扩增的。
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