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公开(公告)号：US5532146A
公开(公告)日：1996-07-02
申请号：US263020
申请日：1994-06-20
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , Y10S435/81
摘要： Methods for sterilization of ligase-based amplification products, including products of the Ligation Amplification Reaction (LAR), involving treatment of nucleic acid after amplification to render such nucleic acid incapable of serving as a template for further amplification. Both random and site-specific addition of sterilizing compounds, and more specifically, photoactivation compounds, render amplification product subsequently unamplifiable. Self-sterilizing primers are also employed allowing for a homogenous detection of ligase-based amplification product.
摘要翻译： 连接酶扩增产物的灭菌方法，包括连接扩增反应（LAR）的产物，涉及在扩增后处理核酸，使得这种核酸不能作为进一步扩增的模板。 杀菌化合物，特别是光活化化合物的随机和位点特异性添加使随后的扩增产物不可扩增。 还使用自消毒引物，允许基于连接酶的扩增产物的均匀检测。
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公开(公告)号：US5565320A
公开(公告)日：1996-10-15
申请号：US131324
申请日：1993-10-04
申请人： George D. Cimino , Lily Lin
发明人： George D. Cimino , Lily Lin
CPC分类号： C12Q1/6848 , C12Q1/686 , C12Q1/703
摘要： A quality control assay to verify adequate treatment of blood product following decontamination with furocoumarins and light. The methods involve measuring the inhibition of template-dependent enzymatic synthesis of nucleic acid following the addition of compounds that add covalently to nucleic acid.
摘要翻译： 一种质量控制测定，用于在用呋喃香豆素和光清除后验证血液制品的充分治疗。 这些方法涉及在加入与核酸共价加成的化合物之后测量对模板依赖的酶合成的抑制。
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3.发明授权Methods for inactivating bacteria in blood preparations with 8-methoxypsoralen 失效用8-甲氧基补骨脂素灭活血液制剂中细菌的方法
公开(公告)号：US5288605A
公开(公告)日：1994-02-22
申请号：US844790
申请日：1992-03-02
IPC分类号： A61J3/00 , A01N1/02 , A61K31/37 , A61K35/14 , A61K41/00 , A61L2/00 , A61M1/36 , A61M37/00 , A61N1/30
CPC分类号： A61K31/37 , A01N1/0215 , A01N1/0242 , A01N1/0294 , A61K41/0019 , A61L2/0011 , A61M1/3616 , A61M1/3683 , A61M1/3686
摘要： Methods and compositions are described for treating contaminants in material intended for in vivo use, and in particular blood and blood products for human use. Contaminants in blood cell preparations are inactivated using 8-methoxypsoralen prior to long term storage and transfusion.
摘要翻译： 描述了用于治疗旨在体内使用的材料中的污染物的方法和组合物，特别是用于人类的血液和血液制品。 血液细胞制剂中的污染物在长期储存和输血之前使用8-甲氧基补骨脂素灭活。
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公开(公告)号：US07025877B1
公开(公告)日：2006-04-11
申请号：US09325599
申请日：1999-06-03
申请人： Serge de Gheldere , Mahmood Mohiuddin , Peyton S. Metzel , George D. Cimino , Derek J. Hei , Michael S. Clarke
发明人： Serge de Gheldere , Mahmood Mohiuddin , Peyton S. Metzel , George D. Cimino , Derek J. Hei , Michael S. Clarke
IPC分类号： B01D15/00
CPC分类号： A61J1/10 , A61L2/0023 , A61L2/0035 , A61L2/0047 , A61L2/007 , A61L2/0082 , A61L2/24 , A61L2/26 , A61L2202/122 , A61L2202/14 , A61L2202/22 , A61M1/0218 , A61M1/0236 , A61M1/0277 , A61M1/3681 , A61M1/3686 , B01D15/00
摘要： Apparatus, systems and methods are disclosed for treating a biological fluid with light. A container of biological fluid is introduced into a fluid treatment chamber where it is contacted with light provided by one or more light sources in proximity to the fluid treatment chamber. A drawer for holding containers of biological fluid introduces the containers into the chamber. Containers for holding the biological fluid are marked by the apparatus to indicate the status of the treatment.
摘要翻译： 公开了用于用光处理生物流体的装置，系统和方法。 将生物流体的容器引入到流体处理室中，在该处理室中，与液体处理室附近的一个或多个光源提供的光接触。 用于容纳生物流体的抽屉将容器引入腔室。 用于保持生物流体的容器由装置标记以指示处理的状态。
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公开(公告)号：US06680025B2
公开(公告)日：2004-01-20
申请号：US10236373
申请日：2002-09-06
IPC分类号： A61L200
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902 , C12Q2523/101 , C12Q2521/531
摘要： A device containing a light source, a sample support and a temperature control compartment. The sample support is positioned to support multiple sample vessels for irradiation by the light source. The temperature control compartment maintains the temperature of the sample vessels within a desired range. The light source, sample support and temperature control compartment are all contained within an opaque housing.
摘要翻译： 包含光源，样品支架和温度控制室的装置。 样品支架定位成支撑多个样品容器用于照射光源。 温度控制室将样品容器的温度保持在所需的范围内。 光源，样品支架和温度控制室都包含在不透明的外壳内。
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公开(公告)号：US06461567B1
公开(公告)日：2002-10-08
申请号：US09878542
申请日：2001-06-11
IPC分类号： A61L200
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902 , C12Q2523/101 , C12Q2521/531
摘要： A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.
摘要翻译： 一种包括光源，样品架和温度控制室的装置。 样品支架相对于光源以固定的关系支撑血管。 温度控制室围绕样品保持器和容器，使得温度控制流体可以有效地将样品容器的温度保持在期望的温度范围内。
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公开(公告)号：US06258319B1
公开(公告)日：2001-07-10
申请号：US09148520
申请日：1998-09-04
IPC分类号： A61L200
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902 , C12Q2523/101 , C12Q2521/531
摘要： A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.
摘要翻译： 一种包括光源，样品架和温度控制室的装置。 样品支架相对于光源以固定的关系支撑血管。 温度控制室围绕样品保持器和容器，使得温度控制流体可以有效地将样品容器的温度保持在期望的温度范围内。
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8.发明授权Identification of allele specific nucleic acid sequences by hybridization with crosslinkable oligonucleotide probes 失效通过与可交联寡核苷酸探针杂交鉴定等位基因特异性核酸序列
公开(公告)号：US5652096A
公开(公告)日：1997-07-29
申请号：US231440
申请日：1994-04-20
申请人： George D. Cimino
发明人： George D. Cimino
CPC分类号： C12Q1/703 , C12Q1/6813 , C12Q1/6816 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6876 , Y10S435/803 , Y10S435/82
摘要： Hybridization method for discriminating between complementarity and partial complementarity of DNA base sequences. Kinetic covalent entrapment of PCR-amplified target DNA is achieved through the use of crosslinkable probes. A crosslinking site is introduced into the target DNA via the PCR amplification process. Problems with renaturation of target DNA in the PCR process and in the hybridization reaction are minimized.
摘要翻译： 用于区分DNA碱基序列的互补性和部分互补性的杂交方法。 通过使用可交联的探针实现PCR扩增的靶DNA的动力学共价捕获。 通过PCR扩增过程将交联位点引入目标DNA。 PCR过程中和杂交反应中靶DNA复性的问题最小化。
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公开(公告)号：US5503721A
公开(公告)日：1996-04-02
申请号：US320126
申请日：1994-10-07
IPC分类号： B01J19/12 , B01L7/00 , C07D493/04 , C07D519/00 , C12Q1/68 , G11C11/412 , H01L27/11
CPC分类号： B01L7/52 , B01J19/123 , B01L7/00 , C07D493/04 , C12Q1/6848 , G11C11/412 , H01L27/11 , Y10S204/902
摘要： A method for activating photoreactive compounds by supporting sample vessels containing a photoreactive compound at a selected temperature and irradiating the sample vessels while holding the sample vessels at a fixed relationship with the irradiating source so as to uniformly irradiate the compound.
摘要翻译： 一种通过在选定温度下支持含有光反应性化合物的样品容器来激活光反应性化合物的方法，并且在将样品容器与照射源保持固定关系的同时照射样品容器，以均匀地照射该化合物。
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公开(公告)号：US5221608A
公开(公告)日：1993-06-22
申请号：US428494
申请日：1989-10-26
IPC分类号： B01L7/00 , C07D311/18 , C07D493/04 , C07D519/00 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/68 , C12Q1/70
CPC分类号： C07D493/04 , B01L7/00 , B01L7/52 , C07D311/18 , C07H21/00 , C12N15/10 , C12P19/34 , C12Q1/6848 , C12Q1/6876 , C12Q1/703 , Y10S435/808 , Y10S436/805
摘要： Method for treating nucleic acid that is to be amplified such that the nucleic acid is, after amplification, subsequently unamplifiable. Nucleic acid amplification is performed in a reaction containing means in the presence of an isopsoralen. The reaction is thereafter irradiated to render the amplified nucleic acid substantially unamplifiable.
摘要翻译： 用于处理待扩增的核酸使得核酸在扩增后随后不可扩增的方法。 在含有补骨脂素的装置中进行核酸扩增。 然后将反应物照射以使扩增的核酸基本上不可扩增。
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