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公开(公告)号：US08470603B2
公开(公告)日：2013-06-25
申请号：US12298591
申请日：2007-04-30
CPC分类号： G01N33/9493 , A61K38/02 , G01N33/15 , G01N33/68 , G01N33/6806 , G01N33/6821 , G01N33/6848 , Y10T436/24 , Y10T436/25125
摘要： The presently disclosed subject matter provides methods for evaluating and characterizing peptides, peptide mixtures, and polypeptide mixtures. More particularly, the presently disclosed subject matter provides methods for evaluating or characterizing complex peptide or polypeptide mixtures comprising glutamic acid, alanine, tyrosine, and lysine, e.g., Copolymer-1 or glatiramer acetate, including, but not limited to, methods of identifying, isolating, quantifying, and purifying amino acids, peptides, polypeptides, and combinations thereof having a diethylamide group instead of a carboxyl group present on the C-terminus. The presently disclosed methods can be used to determine the mole percent of polypeptides having a diethylamide group at a C-terminus thereof and can be used to evaluate one or more properties of a sample of one polypeptide mixture as compared to one or more properties of a different sample of a polypeptide mixture.
摘要翻译： 目前公开的主题提供了用于评估和表征肽，肽混合物和多肽混合物的方法。 更具体地，本发明公开的主题提供了用于评估或表征包含谷氨酸，丙氨酸，酪氨酸和赖氨酸的复合肽或多肽混合物的方法，例如共聚物-1或乙酸格拉默，包括但不限于鉴定， 分离，定量和纯化具有二乙基酰胺基团而不是C末端存在的羧基的氨基酸，肽，多肽及其组合。 目前公开的方法可以用于确定在C末端具有二乙酰胺基团的多肽的摩尔百分比，并且可以用于评估一种多肽混合物的样品的一种或多种性质，与一种或多种性质的 不同样品的多肽混合物。
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公开(公告)号：US07968082B1
公开(公告)日：2011-06-28
申请号：US12011308
申请日：2008-01-25
IPC分类号： A61B5/055 , A61K31/727
CPC分类号： A61K31/727 , Y10S505/844
摘要： Methods for analyzing mixtures of polysaccharides, for example heparin such as enoxaparin are described. In some instances, the mixtures are analyzed using 1D NMR and/or 2D NMR.
摘要翻译： 描述了分析多糖混合物的方法，例如肝素如依诺肝素。 在一些情况下，使用一维NMR和/或二维NMR分析混合物。
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公开(公告)号：US08663999B2
公开(公告)日：2014-03-04
申请号：US13491764
申请日：2012-06-08
申请人： Ian Christopher Parsons , Jonathan Lansing , Daniela Becatti , Scott Bailey , Carlos J. Bosques
发明人： Ian Christopher Parsons , Jonathan Lansing , Daniela Becatti , Scott Bailey , Carlos J. Bosques
CPC分类号： G01N33/68 , G01N33/6848 , G01N2400/38 , G01R33/54
摘要： The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.
摘要翻译： 本公开提供用于表征N-连接聚糖混合物的核磁共振（NMR）方法。 不限于此，本公开的方法可用于表征单糖组成，支化，岩藻糖基化，硫酸化，磷酸化，唾液酸化连接，杂质的存在和/或标记过程的效率（例如，用诸如2-AB的荧光团标记 ）。 在某些实施方案中，可以定量地使用这些方法。 在某些实施方案中，所述方法可与酶消化组合以进一步表征聚糖混合物。
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公开(公告)号：US08216851B2
公开(公告)日：2012-07-10
申请号：US12595940
申请日：2008-04-15
申请人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccati , Scott Bailey , Carlos J. Bosques
发明人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccati , Scott Bailey , Carlos J. Bosques
CPC分类号： G01N33/68 , G01N33/6848 , G01N2400/38 , G01R33/54
摘要： The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.
摘要翻译： 本公开提供用于表征N-连接聚糖混合物的核磁共振（NMR）方法。 不限于此，本公开的方法可用于表征单糖组成，支化，岩藻糖基化，硫酸化，磷酸化，唾液酸化连接，杂质的存在和/或标记过程的效率（例如，用诸如2-AB的荧光团进行标记 ）。 在某些实施方案中，可以定量地使用这些方法。 在某些实施方案中，所述方法可与酶消化组合以进一步表征聚糖混合物。
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5.发明授权Comparative analysis of protein conformations by using 2D NOESY NMR spectra 有权通过使用2D NOESY NMR光谱对蛋白质构象进行比较分析公开(公告)号：US09182467B2
公开(公告)日：2015-11-10
申请号：US12595867
申请日：2008-04-15
IPC分类号： G01N24/08 , G01R33/48 , G01R33/465 , G01R33/56 , G01N24/00 , G01R33/00 , G01R33/46 , G01R33/12
CPC分类号： G01R33/4633 , G01N24/08 , G01N24/087 , G01R33/1269 , G01R33/465 , G01R33/48 , G01R33/5605
摘要： The present disclosure provides a method for determining the relative conformations of a protein provided in different protein preparations, comprising steps of: (i) obtaining a first 2D NOESY NMR spectrum of a first protein preparation; (ii) obtaining a second 2D NOESY NMR spectrum of a second protein preparation; and (iii); determining whether a protein has a different conformation in the first and second protein preparations by comparing one or more cross-peaks in the first 2D NOESY NMR spectrum with one or more corresponding cross-peaks in the second 2D NOESY NMR spectrum.
摘要翻译： 本公开提供了用于确定不同蛋白质制剂中提供的蛋白质的相对构象的方法，包括以下步骤：（i）获得第一蛋白质制剂的第一2D NOESY NMR光谱; （ii）获得第二种蛋白质制剂的第二种二维核磁共振谱; 和（iii）; 通过将第一2D NOESY NMR光谱中的一个或多个交叉峰与第二2D NOESY NMR光谱中的一个或多个相应的交叉峰进行比较来确定蛋白质在第一和第二蛋白质制剂中是否具有不同的构象。
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公开(公告)号：US20090237078A1
公开(公告)日：2009-09-24
申请号：US12298591
申请日：2007-04-30
CPC分类号： G01N33/9493 , A61K38/02 , G01N33/15 , G01N33/68 , G01N33/6806 , G01N33/6821 , G01N33/6848 , Y10T436/24 , Y10T436/25125
摘要： The presently disclosed subject matter provides methods for evaluating and characterizing peptides, peptide mixtures, and polypeptide mixtures. More particularly, the presently disclosed subject matter provides methods for evaluating or characterizing complex peptide or polypeptide mixtures comprising glutamic acid, alanine, tyrosine, and lysine, e.g., Copolymer-1 or glatiramer acetate, including, but not limited to, methods of identifying, isolating, quantifying, and purifying amino acids, peptides, polypeptides, and combinations thereof having a diethylamide group instead of a carboxyl group present on the C-terminus. The presently disclosed methods can be used to determine the mole percent of polypeptides having a diethylamide group at a C-terminus thereof and can be used to evaluate one or more properties of a sample of one polypeptide mixture as compared to one or more properties of a different sample of a polypeptide mixture.
摘要翻译： 目前公开的主题提供了用于评估和表征肽，肽混合物和多肽混合物的方法。 更具体地，本发明公开的主题提供了用于评估或表征包含谷氨酸，丙氨酸，酪氨酸和赖氨酸的复合肽或多肽混合物的方法，例如共聚物-1或乙酸格拉默，包括但不限于鉴定， 分离，定量和纯化具有二乙基酰胺基团而不是C末端存在的羧基的氨基酸，肽，多肽及其组合。 目前公开的方法可以用于确定在C末端具有二乙酰胺基团的多肽的摩尔百分比，并且可以用于评估一种多肽混合物的样品的一种或多种性质，与一种或多种性质的 不同样品的多肽混合物。
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公开(公告)号：US20130069645A1
公开(公告)日：2013-03-21
申请号：US13491764
申请日：2012-06-08
申请人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccatti , Scott Bailey , Carlos J. Bosques
发明人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccatti , Scott Bailey , Carlos J. Bosques
IPC分类号： G01R33/54
CPC分类号： G01N33/68 , G01N33/6848 , G01N2400/38 , G01R33/54
摘要： The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.
摘要翻译： 本公开提供用于表征N-连接聚糖混合物的核磁共振（NMR）方法。 不限于此，本公开的方法可用于表征单糖组成，支化，岩藻糖基化，硫酸化，磷酸化，唾液酸化连接，杂质的存在和/或标记过程的效率（例如，用诸如2-AB的荧光团进行标记 ）。 在某些实施方案中，可以定量地使用这些方法。 在某些实施方案中，所述方法可与酶消化组合以进一步表征聚糖混合物。
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公开(公告)号：US20100279269A1
公开(公告)日：2010-11-04
申请号：US12595940
申请日：2008-04-15
申请人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccati , Scott Bailey , Carlos J. Bosques
发明人： Ian Christopher Parsons , Jonathan Lansing , Daniela Beccati , Scott Bailey , Carlos J. Bosques
CPC分类号： G01N33/68 , G01N33/6848 , G01N2400/38 , G01R33/54
摘要： The present disclosure provides nuclear magnetic resonance (NMR) methods for characterizing mixtures of N-linked glycans. Without limitation, methods of the present disclosure may be useful in characterizing monosaccharide composition, branching, fucosylation, sulfation, phosphorylation, sialylation linkages, presence of impurities and/or efficiency of a labeling procedure (e.g., labeling with a fluorophore such as 2-AB). In certain embodiments, the methods can be used quantitatively. In certain embodiments, the methods can be combined with enzymatic digestion to further characterize glycan mixtures.
摘要翻译： 本公开提供用于表征N-连接聚糖混合物的核磁共振（NMR）方法。 不限于此，本公开的方法可用于表征单糖组成，支化，岩藻糖基化，硫酸化，磷酸化，唾液酸化连接，杂质的存在和/或标记过程的效率（例如，用诸如2-AB的荧光团进行标记 ）。 在某些实施方案中，可以定量地使用这些方法。 在某些实施方案中，所述方法可与酶消化组合以进一步表征聚糖混合物。
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9.发明申请COMPARATIVE ANALYSIS OF PROTEIN CONFORMATIONS BY USING 2D NOESY NMR SPECTRA 有权使用二维核磁共振谱对蛋白质结构进行比较分析公开(公告)号：US20100188084A1
公开(公告)日：2010-07-29
申请号：US12595867
申请日：2008-04-15
IPC分类号： G01R33/48
CPC分类号： G01R33/4633 , G01N24/08 , G01N24/087 , G01R33/1269 , G01R33/465 , G01R33/48 , G01R33/5605
摘要： The present disclosure provides a method for determining the relative conformations of a protein provided in different protein preparations, comprising steps of: (i) obtaining a first 2D NOESY NMR spectrum of a first protein preparation; (ii) obtaining a second 2D NOESY NMR spectrum of a second protein preparation; and (iii); determining whether a protein has a different conformation in the first and second protein preparations by comparing one or more cross-peaks in the first 2D NOESY NMR spectrum with one or more corresponding cross-peaks in the second 2D NOESY NMR spectrum.
摘要翻译： 本公开提供了用于确定不同蛋白质制剂中提供的蛋白质的相对构象的方法，包括以下步骤：（i）获得第一蛋白质制剂的第一2D NOESY NMR光谱; （ii）获得第二种蛋白质制剂的第二种二维核磁共振谱; 和（iii）; 通过将第一2D NOESY NMR光谱中的一个或多个交叉峰与第二2D NOESY NMR光谱中的一个或多个相应的交叉峰进行比较来确定蛋白质在第一和第二蛋白质制剂中是否具有不同的构象。
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