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    Method for the enzymatic preparation of aromatic substances
    6.
    发明授权
    Method for the enzymatic preparation of aromatic substances 失效
    芳香物质酶法制备方法

    公开(公告)号：US5712132A

    公开(公告)日：1998-01-27

    申请号：US574786

    申请日：1995-12-19

    Applicant: Jean Mane ,  Joseph Zucca

    Inventor： Jean Mane ,  Joseph Zucca

    IPC: C12P7/24 ,  C12P17/04 ,  C12P7/42 ,  C12N9/02

    CPC classification number: C12P7/24 ,  C12P17/04

    Abstract: A method for preparing aromatic substances having formula (I), wherein a substrate of formula (II) or an isomer of these precursors, also termed a substrate, in particular selected from those of formula (III), in which formulae R.sub.1 may be a --CH3, --CH2OH, --CHO, --COOH, or (a) radical, R.sub.2 may be a --H, --OH or --O--CH3 radical R.sub.3 may be a --H, --OH or --O--CH3 radical, and R.sub.2 and R.sub.3 may form a methylenedioxy bridge (b), is exposed to the activity of a lipoxygenase. ##STR1##

    Abstract translation: 一种制备具有式（I）的芳族物质的方法，其中式（II）的底物或这些前体的异构体，也称为底物，特别是选自式（III）的那些，其中式R1可以是 -CH 3，-CH 2 OH，-CHO，-COOH或（a）基团，R 2可以是-H，-OH或-O-CH 3基团R 3可以是-H，-OH或-O-CH 3基团，和 R2和R3可以形成亚甲二氧基桥（b），暴露于脂氧合酶的活性。

    System for the production of aromatic molecules in Streptomyces
    7.
    发明授权
    System for the production of aromatic molecules in Streptomyces 有权
    在链霉菌中生产芳香族分子的系统

    公开(公告)号：US08344119B2

    公开(公告)日：2013-01-01

    申请号：US12528000

    申请日：2008-02-20

    Applicant: Fanny Lambert ,  Joseph Zucca ,  Jean Mane

    Inventor： Fanny Lambert ,  Joseph Zucca ,  Jean Mane

    IPC: C07H21/04 ,  C12N1/21 ,  C12N9/04 ,  C12P7/24 ,  C12P7/42 ,  C12P7/22

    CPC classification number: C12P7/42 ,  C12N9/0006 ,  C12P7/22 ,  C12P7/24

    Abstract: A process for the production of natural ferulic acid, coniferyl alcohol and/or vanillin, includes the bio-conversion of eugenol by a bacteria belonging to the Streptomyces genes including at least one nucleotide sequence SEQ ID NO:1 or SEQ ID NO:8 or any nucleotide sequence having at least 70%, preferably 80% and very preferably 90%, identity with the sequence SEQ ID NO:1 or SEQ ID NO:8.

    Abstract translation: 生产天然阿魏酸，松柏醇和/或香草醛的方法包括由属于链霉菌基因的细菌对丁子香酚的生物转化，包括至少一个核苷酸序列SEQ ID NO：1或SEQ ID NO：8或 与序列SEQ ID NO：1或SEQ ID NO：8具有至少70％，优选80％，非常优选90％同一性的任何核苷酸序列。

    Stereoselective Preparation of Gamma-Lactones
    8.
    发明申请
    Stereoselective Preparation of Gamma-Lactones 有权
    立体选择性制备γ-内酯

    公开(公告)号：US20080108116A1

    公开(公告)日：2008-05-08

    申请号：US11666734

    申请日：2005-11-02

    Applicant: Joseph Zucca

    Inventor： Joseph Zucca

    IPC: C12P17/04

    CPC classification number: C07D307/33 ,  C12P17/04

    Abstract: Method for stereoselectively preparing a (R) or (S) gamma-lactone consists in microbially biosynthesising gamma-lactone, wherein the biosynthesis is carried out from a substrate selected from C5 to C20 fatty acids with the aid of a strain selected from those enabling a stereoselective hydroxylation in C4 of the substrate, in particular Aspergollus sp or Mortierella sp. The biosynthesis of the gamma-lactone and the use thereof in perfumery and for food flavouring agents are also disclosed.

    Abstract translation: 用于立体选择性制备（R）或（S）γ-内酯的方法在于微生物合成γ-内酯，其中所述生物合成是从选自C5至C20脂肪酸的底物借助于选自以下的菌株进行的： 底物C4中的立体选择性羟基化，特别是Aspergollus sp或Mortierella sp。 还公开了γ-内酯的生物合成及其在香料和食品调味剂中的用途。

    Biochemical process for preparing aromatic substances
    9.
    发明授权
    Biochemical process for preparing aromatic substances 失效
    芳香物质生化工艺

    公开(公告)号：US5861286A

    公开(公告)日：1999-01-19

    申请号：US860876

    申请日：1997-09-25

    Applicant: Jean Mane ,  Joseph Zucca

    Inventor： Jean Mane ,  Joseph Zucca

    IPC: C07H15/203 ,  C07C45/28 ,  C07C47/54 ,  C07C47/565 ,  C07C47/575 ,  C12P7/24 ,  C12P19/44 ,  C07C47/58

    CPC classification number: C12P7/24 ,  C12P19/44

    Abstract: The present invention relates to a process for the preparation of aromatic substances having formula (I), characterized in that a substrate having formula (II) is subjected to an oxydation in the presence of at least one protein and at least one metal ion, formula wherein R.sub.1 may be a radical --H, --CH.sub.3, --CH.sub.2 OH, --CHO, --COOH, --OCH.sub.3, or --COO--CH(COOH)--CH.sub.2 --C.sub.6 H.sub.3 (OH).sub.2 ; R.sub.2 may be a radical --H, --OH, or --OCH.sub.3 ; R.sub.3 may be a radical --H, --OH, --OCH.sub.3, or O-glucosid; R.sub.4 may be a radical --H, --OH, or --OCH.sub.3. ##STR1##

    Abstract translation: PCT No.PCT / FR96 / 00082 Sec。 371日期：1997年9月25日 102（e）1997年9月25日PCT PCT 1996年1月18日PCT公布。 WO96 / 22381 PCT出版物 日期：1996年7月25日本发明涉及一种制备具有式（I）的芳香族物质的方法，其特征在于：在至少一种蛋白质存在下使具有式（II）的底物进行氧化反应 一种金属离子，其中R 1可以是基团-H，-CH 3，-CH 2 OH，-CHO，-COOH，-OCH 3或-COO-CH（COOH）-CH 2 -C 6 H 3（OH）2; R 2可以是基团-H，-OH或-OCH 3; R3可以是自由基-H，-OH，-OCH3或O-葡萄糖; R 4可以是基团-H，-OH或-OCH 3。

    Stereoselective preparation of gamma-lactones
    10.
    发明授权
    Stereoselective preparation of gamma-lactones 有权
    γ-内酯的立体选择性制备

    公开(公告)号：US07863023B2

    公开(公告)日：2011-01-04

    申请号：US11666734

    申请日：2005-11-02

    Applicant: Joseph Zucca ,  Jean Mane

    Inventor： Joseph Zucca ,  Jean Mane

    IPC: C12P17/04

    CPC classification number: C07D307/33 ,  C12P17/04

    Abstract: Method for stereoselectively preparing a (R) or (S) gamma-lactone consists in microbially biosynthesizing gamma-lactone, wherein the biosynthesis is carried out from a substrate selected from C5 to C20 fatty acids with the aid of a strain selected from those enabling a stereoselective hydroxylation in C4 of the substrate, in particular Aspergollus sp or Mortierella sp. The biosynthesis of the gamma-lactone and the use thereof in perfumery and for food flavouring agents are also disclosed.

    Abstract translation: 用于立体选择性制备（R）或（S）γ-内酯的方法在于微生物合成γ-内酯，其中所述生物合成是在选自C5至C20脂肪酸的底物的帮助下从选自以下的菌株中进行的： 底物C4中的立体选择性羟基化，特别是Aspergollus sp或Mortierella sp。 还公开了γ-内酯的生物合成及其在香料和食品调味剂中的用途。

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