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    Uses of Dinucleotide Polyphosphate Derivatives
    3.
    发明申请
    Uses of Dinucleotide Polyphosphate Derivatives 审中-公开
    二核苷酸多磷酸衍生物的用途

    公开(公告)号：US20080319184A1

    公开(公告)日：2008-12-25

    申请号：US11883661

    申请日：2006-02-01

    申请人： Andrew David Miller ,  Michael Wright ,  Julian Alexander Tanner ,  Natalya Lozovaya

    发明人： Andrew David Miller ,  Michael Wright ,  Julian Alexander Tanner ,  Natalya Lozovaya

    IPC分类号： C07H21/02

    CPC分类号： A61K31/7084 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04

    摘要： The present invention provides the use of analogues and derivatives of dinucleoside polyphosphates with formula (I) or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for use in one or more of: the treatment of ischemia, inducing ischemic tolerance, modulating cerebral ischemia, to delay the onset of a hypoxic depolarisation stage when ischemic events are initiated; as a neurological protection agent; as a tissue protection agent; the treatment of pain; and the treatment of inflammation, wherein X, is selected from wherein X1 and X2 are independently selected from H, Cl, Br and F; each Y is independently selected from S and O; each Z is independently selected from —CX3X4—,—NH—,—O—; wherein X3 and X4 are selected from H, Cl, Br and F; B1 and B2 are independently selected from adenine, guanine, xanthine, thymine, uracil, cytosine and inosine; S1 and S2 are independently selected from ribose, open chain ribose, 2′-deoxyribose, 3′deoxyribose and arabinofuranoside. V is selected from 0, 1, 2, 3, 4 and 5; W is selected from 0, 1, 2, 3, 4 and 5; and V plus W is an integer from 2 to 6.

    摘要翻译： 本发明提供了式（I）的二核苷多聚磷酸酯类似物和衍生物或其药学上可接受的盐在制备用于以下一种或多种中的一种或多种药物中的用途：治疗缺血，诱导缺血耐受，调节脑 缺血，当缺血事件发生时延迟缺氧去极化阶段的发作; 作为神经保护剂; 作为组织保护剂; 治疗疼痛; 和炎症的治疗，其中X选自其中X 1和X 2独立地选自H，Cl，Br和F; 每个Y独立地选自S和O; 每个Z独立地选自-CX 3 X 4 - ， - NH - ， - O-; 其中X3和X4选自H，Cl，Br和F; B1和B2独立地选自腺嘌呤，鸟嘌呤，黄嘌呤，胸腺嘧啶，尿嘧啶，胞嘧啶和肌苷; S1和S2独立地选自核糖，开链核糖，2'-脱氧核糖，3'-脱氧核糖和阿拉伯呋喃糖苷。 V选自0，1，2，3，4和5; W选自0，1，2，3，4和5; V加W为2〜6的整数。

    Method for constructing and modifying large DNA molecules
    4.
    发明授权
    Method for constructing and modifying large DNA molecules 有权
    构建和修饰大DNA分子的方法

    公开(公告)号：US07781190B2

    公开(公告)日：2010-08-24

    申请号：US10892579

    申请日：2004-07-16

    申请人： Jian-Dong Huang ,  Xin-Mei Zhang ,  Julian Alexander Tanner

    发明人： Jian-Dong Huang ,  Xin-Mei Zhang ,  Julian Alexander Tanner

    IPC分类号： C12N15/64 ,  C12N15/70

    CPC分类号： C12N15/10

    摘要： This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.

    摘要翻译： 本发明提供了一种组合重叠DNA分子的方法，包括：（a）提供第一和第二DNA片段，第一DNA片段具有与第二DNA片段中的区域同源的区域; （b）用选择标记标记第一个DNA片段; （c）将第一DNA序列克隆到检索载体中以形成DNA载体复合物; （d）使DNA-载体复合物线性化; 和（e）使用同源重组将来自DNA-载体复合物的第一个DNA片段插入第二个DNA片段以形成组合的DNA分子; 和（f）除去选择性标记，从而产生组合的DNA分子。 本发明还提供了用于检索并将选定的DNA分子插入靶DNA分子的载体。