公开(公告)号：US20130264221A1

公开(公告)日：2013-10-10

申请号：US13994348

申请日：2011-12-16

申请人： Min Gon Kim ,  Jun Hyoung Ahn ,  Yun Ju Sung ,  Yong Beom Shin ,  Hyo Arm Joung

发明人： Min Gon Kim ,  Jun Hyoung Ahn ,  Yun Ju Sung ,  Yong Beom Shin ,  Hyo Arm Joung

IPC分类号： G01N27/327 ,  A61B5/1486

CPC分类号： G01N27/3278 ,  A61B5/1486 ,  B82Y5/00 ,  B82Y15/00 ,  G01N27/3275 ,  G01N33/54346 ,  G01N33/54366

摘要： A membrane electrode includes a novel sensor combining a filtering function of a membrane and a signal measuring ability of an electrode. A target material may be measured by filtration through the membrane. A small amount of target materials may be detected with high sensitivity using an amplified electrical signal by increasing electrical conductivity by reducing metal ions on the membrane, and thus the target material may be subject to quantitative analysis. In addition, only a target material selectively binding to a receptor may be filtrated by passing a sample through the membrane after a receptor material is fixed to the electrode, and thus may be used to detect an electrical signal. In addition, the sensor may measure a signal in various methods such as electrical conductivity, impedance, etc.

摘要翻译： 膜电极包括结合膜的过滤功能和电极的信号测量能力的新型传感器。 目标材料可以通过膜过滤来测量。 使用放大的电信号，可以通过降低膜上的金属离子来提高导电性，可以以高灵敏度检测少量的目标材料，因此可以对靶材进行定量分析。 此外，只有选择性地结合受体的靶材料可以在将受体材料固定到电极之后通过样品通过膜来过滤，因此可以用于检测电信号。 此外，传感器可以以各种方法测量信号，例如导电性，阻抗等。

公开(公告)号：US09465003B2

公开(公告)日：2016-10-11

申请号：US13994348

申请日：2011-12-16

申请人： Min Gon Kim ,  Jun Hyoung Ahn ,  Yun Ju Sung ,  Yong Beom Shin ,  Hyo Arm Joung

发明人： Min Gon Kim ,  Jun Hyoung Ahn ,  Yun Ju Sung ,  Yong Beom Shin ,  Hyo Arm Joung

IPC分类号： G01N27/327 ,  G01N33/543 ,  A61B5/1486 ,  B82Y5/00 ,  B82Y15/00

CPC分类号： G01N27/3278 ,  A61B5/1486 ,  B82Y5/00 ,  B82Y15/00 ,  G01N27/3275 ,  G01N33/54346 ,  G01N33/54366

摘要： A membrane electrode includes a novel sensor combining a filtering function of a membrane and a signal measuring ability of an electrode. A target material may be measured by filtration through the membrane. A small amount of target materials may be detected with high sensitivity using an amplified electrical signal by increasing electrical conductivity by reducing metal ions on the membrane, and thus the target material may be subject to quantitative analysis. In addition, only a target material selectively binding to a receptor may be filtrated by passing a sample through the membrane after a receptor material is fixed to the electrode, and thus may be used to detect an electrical signal. In addition, the sensor may measure a signal in various methods such as electrical conductivity, impedance, etc.

摘要翻译： 膜电极包括结合膜的过滤功能和电极的信号测量能力的新型传感器。 目标材料可以通过膜过滤来测量。 使用放大的电信号，可以通过降低膜上的金属离子来提高导电性，可以以高灵敏度检测少量的目标材料，因此可以对靶材进行定量分析。 此外，只有选择性地结合受体的靶材料可以在将受体材料固定到电极之后通过样品通过膜来过滤，因此可以用于检测电信号。 此外，传感器可以以各种方法测量信号，例如导电性，阻抗等。

公开(公告)号：US20110201027A1

公开(公告)日：2011-08-18

申请号：US12933114

申请日：2009-03-18

申请人： Min Gon Kim ,  Yong Beom Shin ,  Bong Hyun Chung ,  Jun Hyoung Ahn

发明人： Min Gon Kim ,  Yong Beom Shin ,  Bong Hyun Chung ,  Jun Hyoung Ahn

IPC分类号： G01N33/53 ,  C12M1/34 ,  G01N1/28

CPC分类号： G01N33/5438

摘要： The present invention relates to a biosensor capable of a trace amount of sample and a fabrication method thereof. More specifically, the invention relates to a method for fabricating a biosensor, the method comprising: immobilizing a receptor molecule, which binds selectively to a target substance, on an electrically insulated nano-electrode chip; binding an enzyme to the receptor molecule; and treating the bound enzyme with metal ions and depositing the metal ions on the nano-electrode surface, and to a biosensor fabricated thereby. According to the invention, a precipitate is produced on the nano-electrode surface by a precipitation between the enzyme and the metal ions, and the produced precipitate electrically connects the nano-electrodes together, thereby increasing electrical conductivity. Thus, the invention is useful for quantitative analysis of trace amounts and/or various concentrations of target substances.

摘要翻译： 本发明涉及能够追踪微量样品的生物传感器及其制造方法。 更具体地说，本发明涉及一种制造生物传感器的方法，该方法包括：在电绝缘的纳米电极芯片上固定与靶物质选择性结合的受体分子; 将酶与受体分子结合; 并用金属离子处理结合的酶并将金属离子沉积在纳米电极表面上，以及由其制造的生物传感器。 根据本发明，通过酶和金属离子之间的沉淀在纳米电极表面上产生沉淀物，并且所产生的沉淀物将纳米电极电连接在一起，从而增加导电性。 因此，本发明可用于痕量和/或各种浓度的目标物质的定量分析。