公开(公告)号：US20140256563A1

公开(公告)日：2014-09-11

申请号：US14119266

申请日：2012-05-22

申请人： Huijuan Luo ,  Guanyu Ji ,  Hui Jiang ,  Mingzhi Wu ,  Jihua Sun ,  Renhua Wu ,  Junwen Wang ,  Fei Gao

发明人： Huijuan Luo ,  Guanyu Ji ,  Hui Jiang ,  Mingzhi Wu ,  Jihua Sun ,  Renhua Wu ,  Junwen Wang ,  Fei Gao

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/06 ,  C12Q2523/125 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/159

摘要： Provided is a high throughput methylation detection method, particularly a combined sequence capture and bisulfite sequencing method. The method accurately and effectively analyzes the methylation status of the target area in several samples simultaneously, lowers the difficulty of probe design, enhances operation and application feasibility, and enables high throughput methylation detection of high accuracy on interested target sequences and areas in a complete genome. The method is targeted and conserves energy and time.

摘要翻译： 提供了高通量甲基化检测方法，特别是组合的序列捕获和亚硫酸氢盐测序方法。 该方法同时精确有效地分析了几个样本中目标区域的甲基化状态，降低了探针设计的难度，提高了操作和应用的可行性，并且使得在完整基因组中有兴趣的靶序列和区域具有高准确度的高通量甲基化检测 。 该方法是针对性的，节约能源和时间。

公开(公告)号：US08323473B2

公开(公告)日：2012-12-04

申请号：US10996218

申请日：2004-11-23

申请人： Yuanfeng Luo ,  Renwei Yuan ,  Kelvin Junwen Wang ,  Bin Wei ,  Michael Scott Lamphere

发明人： Yuanfeng Luo ,  Renwei Yuan ,  Kelvin Junwen Wang ,  Bin Wei ,  Michael Scott Lamphere

IPC分类号： B23H5/00 ,  B23H7/06 ,  B23H7/18

CPC分类号： B23H7/16 ,  B23H1/02

摘要： A method for monitoring machining in an electroerosion assembly having a power supply and an electrode arranged across a gap from a workpiece, includes measuring a voltage at a point in a voltage waveform after a time delay td of one half of a pulse width of the voltage waveform. The measurements are repeated for multiple pulses of the voltage waveform to obtain multiple voltages, each of the voltages corresponding to a point in respective pulses. The voltages are averaged to obtain an average voltage, which is compared with at least one threshold voltage, to determine whether the machining is in control. A control signal is generated if the comparison indicates that the process is not in control, the control signal being configured to regulate an operating parameter of the power supply, and the control signal is supplied to the power supply, if generated.

摘要翻译： 一种用于在具有电源和跨工件间隙布置的电极的电腐蚀组件中监测加工的方法，包括在所述电压的脉冲宽度的一半的时间延迟td之后测量电压波形中的点处的电压 波形 对电压波形的多个脉冲重复测量以获得多个电压，每个电压对应于各个脉冲中的点。 将电压平均以获得与至少一个阈值电压进行比较的平均电压，以确定加工是否处于控制状态。 如果比较指示处理不在控制中，则控制信号被产生，控制信号被配置为调节电源的操作参数，并且如果产生，则控制信号被提供给电源。

公开(公告)号：US09133513B2

公开(公告)日：2015-09-15

申请号：US14119266

申请日：2012-05-22

申请人： Huijuan Luo ,  Guanyu Ji ,  Hui Jiang ,  Mingzhi Wu ,  Jihua Sun ,  Renhua Wu ,  Junwen Wang ,  Fei Gao

发明人： Huijuan Luo ,  Guanyu Ji ,  Hui Jiang ,  Mingzhi Wu ,  Jihua Sun ,  Renhua Wu ,  Junwen Wang ,  Fei Gao

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C40B50/06

CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6869 ,  C40B50/06 ,  C12Q2523/125 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/159

摘要： Provided is a high throughput methylation detection method, particularly a combined sequence capture and bisulfite sequencing method. The method accurately and effectively analyzes the methylation status of the target area in several samples simultaneously, lowers the difficulty of probe design, enhances operation and application feasibility, and enables high throughput methylation detection of high accuracy on interested target sequences and areas in a complete genome. The method is targeted and conserves energy and time.

摘要翻译： 提供了高通量甲基化检测方法，特别是组合的序列捕获和亚硫酸氢盐测序方法。 该方法同时精确有效地分析了几个样本中目标区域的甲基化状态，降低了探针设计的难度，提高了操作和应用的可行性，并且使得在完整基因组中有兴趣的靶序列和区域具有高准确度的高通量甲基化检测 。 该方法是针对性的，节约能源和时间。