公开(公告)号：US07081342B2

公开(公告)日：2006-07-25

申请号：US10654545

申请日：2003-09-03

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Kelly Burkhart ,  David E. Gorcyca ,  Mike Roof

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Kelly Burkhart ,  David E. Gorcyca ,  Mike Roof

IPC分类号： C12Q1/68

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

公开(公告)号：US06660513B2

公开(公告)日：2003-12-09

申请号：US10143186

申请日：2002-05-09

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

IPC分类号： C12N700

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation.

公开(公告)号：US20110104201A1

公开(公告)日：2011-05-05

申请号：US12389558

申请日：2009-02-20

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N7/00 ,  A61P37/02 ,  A61P31/12

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

摘要翻译： 提供基本上无毒的形式的非典型猪繁殖与呼吸综合征（PRRS）病毒和相应的疫苗，其由PRRS的毒性形式的细胞培养传代产生。 所得到的无毒非典型PRRS病毒可用作疫苗，因为通过接种宿主动物引起PRRS特异性抗体应答，从而赋予先前已知的PRRS病毒株和新分离的非典型PRRS病毒株的有效免疫。 优选的传代技术确保病毒在整个过程中基本上保持在对数生长阶段，这使得实现衰减所需的时间最小化。 本发明还提供可区分感染野外毒株的动物和PRRSV减毒菌株的诊断测试方法。

公开(公告)号：US20090047286A1

公开(公告)日：2009-02-19

申请号：US12171992

申请日：2008-07-11

申请人： MARIE RENE GRAMER ,  KELLY LAGER ,  WENJUN MA ,  JUERGEN RICHT ,  AMY VINCENT

发明人： MARIE RENE GRAMER ,  KELLY LAGER ,  WENJUN MA ,  JUERGEN RICHT ,  AMY VINCENT

IPC分类号： A61K39/395 ,  C12N7/00 ,  A61K35/76 ,  C07K14/00 ,  A61K38/00 ,  C12N9/14 ,  A01K67/027 ,  A61K39/00 ,  A61P31/00 ,  A61K31/7088 ,  C12Q1/70 ,  A61K38/43 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C07K16/18

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N9/2402 ,  C12N2760/16122 ,  C12Y302/01018 ,  G01N33/5088 ,  G01N33/56983 ,  G01N33/6854 ,  G01N2333/11

摘要： The present invention provides influenza A viruses that include a hemagglutinin subtype H2, a neuraminidase subtype N3, or the combination thereof. Included in the present invention are H2 hemagglutinins and N3 neuraminidases, and the polynucleotides encoding the polypeptides. Antibody to the polypeptides, and methods of using the viruses, polypeptides, polynucleotides, and antibodies are also provided.

摘要翻译： 本发明提供了包含血凝素亚型H2，神经氨酸酶亚型N3或其组合的甲型流感病毒。 本发明中包括H2血细胞凝集素和N3神经氨酸酶，以及编码多肽的多核苷酸。 还提供了多肽的抗体，以及使用病毒，多肽，多核苷酸和抗体的方法。

公开(公告)号：US08747859B2

公开(公告)日：2014-06-10

申请号：US12389558

申请日：2009-02-20

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N7/00 ,  A61P37/02 ,  A61P31/12

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

公开(公告)号：US08741309B2

公开(公告)日：2014-06-03

申请号：US11459542

申请日：2006-07-24

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N7/00 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

摘要翻译： 提供基本上无毒的形式的非典型猪繁殖与呼吸综合征（PRRS）病毒和相应的疫苗，其由PRRS的毒性形式的细胞培养传代产生。 所得到的无毒非典型PRRS病毒可用作疫苗，因为通过接种宿主动物引起PRRS特异性抗体应答，从而赋予先前已知的PRRS病毒株和新分离的非典型PRRS病毒株的有效免疫。 优选的传代技术确保病毒在整个过程中基本上保持在对数生长阶段，这使得实现衰减所需的时间最小化。 本发明还提供可区分感染野外毒株的动物和PRRSV减毒菌株的诊断测试方法。

公开(公告)号：US08491916B2

公开(公告)日：2013-07-23

申请号：US11459542

申请日：2006-07-24

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David E. Gorcyca

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N7/00 ,  C12N15/86

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

公开(公告)号：US08084594B2

公开(公告)日：2011-12-27

申请号：US12171992

申请日：2008-07-11

申请人： Marie Rene Gramer ,  Kelly Lager ,  Wenjun Ma ,  Juergen Richt ,  Amy Vincent

发明人： Marie Rene Gramer ,  Kelly Lager ,  Wenjun Ma ,  Juergen Richt ,  Amy Vincent

IPC分类号： C07H21/02 ,  C12N15/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  C12N9/2402 ,  C12N2760/16122 ,  C12Y302/01018 ,  G01N33/5088 ,  G01N33/56983 ,  G01N33/6854 ,  G01N2333/11

摘要： The present invention provides influenza A viruses that include a hemagglutinin subtype H2, a neuraminidase subtype N3, or the combination thereof. Included in the present invention are H2 hemagglutinins and N3 neuraminidases, and the polynucleotides encoding the polypeptides. Antibody to the polypeptides, and methods of using the viruses, polypeptides, polynucleotides, and antibodies are also provided.

摘要翻译： 本发明提供了包含血凝素亚型H2，神经氨酸酶亚型N3或其组合的甲型流感病毒。 本发明中包括H2血细胞凝集素和N3神经氨酸酶，以及编码多肽的多核苷酸。 还提供了多肽的抗体，以及使用病毒，多肽，多核苷酸和抗体的方法。

公开(公告)号：US20070042000A1

公开(公告)日：2007-02-22

申请号：US11459542

申请日：2006-07-24

申请人： William Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David Gorcyca

发明人： William Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Mike Roof ,  Kelly Burkhart ,  David Gorcyca

IPC分类号： A61K39/12 ,  C07H21/04 ,  C12N7/00 ,  C12N15/86

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.

摘要翻译： 提供基本上无毒的形式的非典型猪繁殖与呼吸综合征（PRRS）病毒和相应的疫苗，其由PRRS的毒性形式的细胞培养传代产生。 所得到的无毒非典型PRRS病毒可用作疫苗，因为通过接种宿主动物引起PRRS特异性抗体应答，从而赋予先前已知的PRRS病毒株和新分离的非典型PRRS病毒株的有效免疫。 优选的传代技术确保病毒在整个过程中基本上保持在对数生长阶段，这使得实现衰减所需的时间最小化。 本发明还提供可区分感染野外毒株的动物和PRRSV减毒菌株的诊断测试方法。

公开(公告)号：US06641819B2

公开(公告)日：2003-11-04

申请号：US09981282

申请日：2001-10-18

申请人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Kelly Burkhart ,  David E. Gorcyca ,  Mike Roof

发明人： William L. Mengeling ,  Ann Vorwald ,  Kelly Lager ,  Kelly Burkhart ,  David E. Gorcyca ,  Mike Roof

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N7/00 ,  C12N2770/10022 ,  C12N2770/10034 ,  C12N2770/10061 ,  C12N2770/10064 ,  Y10S424/815

摘要： Substantially avirulent forms of atypical porcine reproductive and respiratory syndrome (PRRS) virus and corresponding vaccines are provided which result from cell culture passaging of virulent forms of PRRS. The resultant avirulent atypical PRRS virus is useful as a vaccine in that PRRS specific antibody response is elicited by inoculation of host animals, thereby conferring effective immunity against both previously known strains of PRRS virus and newly isolated atypical PRRS virus strains. The preferred passaging technique ensures that the virus remains in a logarithmic growth phase substantially throughout the process, which minimizes the time required to achieve attenuation. The present invention also provides diagnostic testing methods which can differentiate between animals infected with field strains and attenuated strains of PRRSV.