公开(公告)号：US08008028B2

公开(公告)日：2011-08-30

申请号：US12225477

申请日：2007-03-13

Applicant: Kazuta Yasui ,  Fumiya Hirayama ,  Rika Furuta ,  Nobuki Matsuyama ,  Yoshitaka Kojima ,  Toru Miyazaki ,  Hisami Ikeda ,  Yoshihisa Watanabe

Inventor： Kazuta Yasui ,  Fumiya Hirayama ,  Rika Furuta ,  Nobuki Matsuyama ,  Yoshitaka Kojima ,  Toru Miyazaki ,  Hisami Ikeda ,  Yoshihisa Watanabe

IPC: G01N33/53 ,  C12N15/63

CPC classification number: G01N33/6854 ,  C12N2510/00 ,  G01N33/564 ,  G01N2333/70539 ,  G01N2800/22

Abstract: A panel cell for detecting anti-HNA antibody is disclosed. The panel cell is obtained by introducing a DNA coding for an HNA antigen corresponding to the anti-HNA antibody into a cell so as to enable the expression of the DNA under the condition for use in the detection procedure, wherein the cell for DNA introduction exhibits no detectable reaction with anti-HLA-ABC antibody, anti-HLA-DR antibody, anti-HLA-DQ antibody, anti-HLA-DP antibody, anti-HNA-1 antibody, anti-HNA-2a antibody, anti-HNA-3a antibody, anti-HNA-4 antibody, anti-HNA-5 antibody, and serum from normal subject, in the detection procedure. The panel cell allows accurate and rapid detection of granulocyte antibody.

Abstract translation: 公开了一种用于检测抗-HNA抗体的细胞。 通过将编码对应于抗HNA抗体的HNA抗原的DNA导入细胞，使其能够在用于检测步骤的条件下使DNA表达，其中DNA引入的细胞显示出来 抗HLA-ABC抗体，抗HLA-DR抗体，抗HLA-DQ抗体，抗HLA-DP抗体，抗HNA-1抗体，抗HNA-2a抗体，抗HNA- 3a抗体，抗HNA-4抗体，抗-HNA-5抗体，以及来自正常受试者的血清。 面板细胞允许准确和快速检测粒细胞抗体。

公开(公告)号：US20090208978A1

公开(公告)日：2009-08-20

申请号：US12225477

申请日：2007-03-13

Applicant: Kazuta Yasui ,  Fumiya Hirayama ,  Rika Furuta ,  Nobuki Matsuyama ,  Yoshitaka Kojima ,  Toru Miyazaki ,  Hisami Ikeda ,  Yoshihisa Watanabe

Inventor： Kazuta Yasui ,  Fumiya Hirayama ,  Rika Furuta ,  Nobuki Matsuyama ,  Yoshitaka Kojima ,  Toru Miyazaki ,  Hisami Ikeda ,  Yoshihisa Watanabe

IPC: G01N33/567 ,  C12N15/09 ,  C12N15/07 ,  C12N5/06 ,  C12N5/08

CPC classification number: G01N33/6854 ,  C12N2510/00 ,  G01N33/564 ,  G01N2333/70539 ,  G01N2800/22

Abstract: A panel cell for detecting anti-HNA antibody is disclosed. The panel cell is obtained by introducing a DNA coding for an HNA antigen corresponding to the anti-HNA antibody into a cell so as to enable the expression of the DNA under the condition for use in the detection procedure, wherein the cell for DNA introduction exhibits no detectable reaction with anti-HLA-ABC antibody, anti-HLA-DR antibody, anti-HLA-DQ antibody, anti-HLA-DP antibody, anti-HNA-1 antibody, anti-HNA-2a antibody, anti-HNA-3a antibody, anti-HNA-4 antibody, anti-HNA-5 antibody, and serum from normal subject, in the detection procedure. The panel cell allows accurate and rapid detection of granulocyte antibody.

Abstract translation: 公开了一种用于检测抗-HNA抗体的细胞。 通过将编码对应于抗HNA抗体的HNA抗原的DNA导入细胞，使其能够在用于检测步骤的条件下使DNA表达，其中DNA引入的细胞显示出来 抗HLA-ABC抗体，抗HLA-DR抗体，抗HLA-DQ抗体，抗HLA-DP抗体，抗HNA-1抗体，抗HNA-2a抗体，抗HNA- 3a抗体，抗HNA-4抗体，抗-HNA-5抗体，以及来自正常受试者的血清。 面板细胞允许准确和快速检测粒细胞抗体。