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公开(公告)号：US07473769B2
公开(公告)日：2009-01-06
申请号：US11222641
申请日：2005-09-08
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic property as high as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these genes into animal cells successfully conferred desiccation resistance to the cells.
摘要翻译： 本发明的目的是提供编码昆虫耐干燥性蛋白质的多核苷酸及其用途。 在干燥状态下从Polypedilum vanderplanki幼虫生产cDNA文库，构建了P.vanderplanki EST数据库，分离了编码LEA蛋白的基因。 这导致了三种类型的编码LEA样蛋白的新基因的成功分离。 对从每个基因推导的蛋白质进行二级结构预测和基序搜索时，所有三种蛋白质都具有富含α-螺旋的结构和LEA_4基序，这是LEA蛋白质的特征。 此外，由这些基因合成的重组蛋白即使在沸腾时也是热溶性的，因此这些蛋白质具有与植物LEA蛋白一样高的亲水性。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些基因导入动物细胞成功地赋予细胞干燥抗性。
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公开(公告)号：US20090124004A1
公开(公告)日：2009-05-14
申请号：US12313645
申请日：2008-11-21
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3.) When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA 1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.
摘要翻译： 本发明的目的是提供编码昆虫耐干燥性蛋白质的多核苷酸及其用途。 在干燥状态下从Polypedilum vanderplanki幼虫生产cDNA文库，构建了P.vanderplanki EST数据库，分离了编码LEA蛋白的基因。 这导致了三种类型的编码LEA样蛋白的新基因（PvLEA1，PvLEA2和PvLEA3）的成功分离。当对从每个基因推导的蛋白进行二级结构预测和基序搜索时，所有三种蛋白都具有α 富含螺旋的结构和LEA_4基序，是LEA蛋白的特征。 此外，由PvLEA1,2和3基因合成的重组蛋白即使在沸腾时也是热溶性的，因此PvLEA1,2和3蛋白具有高亲水性以及植物LEA蛋白。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些PvLEA 1,2和3基因引入动物细胞成功地赋予细胞干燥抗性。 本发明提供了从昆虫分离的LEA基因的第一个实例。
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公开(公告)号：US20110281349A1
公开(公告)日：2011-11-17
申请号：US13103806
申请日：2011-05-09
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3.) When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA 1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.
摘要翻译： 本发明的目的在于提供编码昆虫抗干扰性蛋白质的多核苷酸及其使用方法，从干燥状态的Polypedilum vanderplanki幼虫生产cDNA文库，构建P.vanderplanki EST数据库，分离编码LEA蛋白质的基因。 这导致了三种类型的编码LEA样蛋白的新基因（PvLEA1，PvLEA2和PvLEA3）的成功分离。当对从每个基因推导的蛋白进行二级结构预测和基序搜索时，所有三种蛋白都具有α 富含螺旋的结构和LEA_4基序，是LEA蛋白的特征。 此外，由PvLEA1,2和3基因合成的重组蛋白即使在沸腾时也是热溶性的，因此PvLEA1,2和3蛋白具有高亲水性以及植物LEA蛋白。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些PvLEA 1,2和3基因引入动物细胞成功地赋予细胞干燥抗性。 本发明提供了从昆虫分离的LEA基因的第一个实例。
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公开(公告)号：US08173790B2
公开(公告)日：2012-05-08
申请号：US13103806
申请日：2011-05-09
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3.) When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA 1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.
摘要翻译： 本发明的目的在于提供编码昆虫抗干扰性蛋白质的多核苷酸及其使用方法，从干燥状态的Polypedilum vanderplanki幼虫生产cDNA文库，构建P.vanderplanki EST数据库，分离编码LEA蛋白质的基因。 这导致了三种类型的编码LEA样蛋白的新基因（PvLEA1，PvLEA2和PvLEA3）的成功分离。当对从每个基因推导的蛋白进行二级结构预测和基序搜索时，所有三种蛋白都具有α 富含螺旋的结构和LEA_4基序，是LEA蛋白的特征。 此外，由PvLEA1,2和3基因合成的重组蛋白即使在沸腾时也是热溶性的，因此PvLEA1,2和3蛋白具有高亲水性以及植物LEA蛋白。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些PvLEA 1,2和3基因引入动物细胞成功地赋予细胞干燥抗性。 本发明提供了从昆虫分离的LEA基因的第一个实例。
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公开(公告)号：US20060059583A1
公开(公告)日：2006-03-16
申请号：US11222641
申请日：2005-09-08
IPC分类号： A01H1/00 , A01N65/00 , C07K14/435 , C07H21/04 , C12N5/04
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins (PvLEA1, PvLEA2, and PvLEA3). When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moerover, the recombinant proteins synthesized from PvLEA1, 2 and 3 genes were heat soluble even when boiling, so that PvLEA1, 2 and 3 proteins have highly hydrophilic property as well as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these PvLEA1, 2 and 3 genes into animal cells successfully conferred desiccation resistance to the cells. The present invention provides the first example of LEA genes isolated from insects.
摘要翻译： 本发明的目的是提供编码昆虫耐干燥性蛋白质的多核苷酸及其用途。
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公开(公告)号：US07939647B2
公开(公告)日：2011-05-10
申请号：US12313645
申请日：2008-11-21
CPC分类号： C07K14/43563
摘要： An objective of the present invention is to provide polynucleotides encoding insect desiccation resistance proteins, and uses thereof. cDNA libraries were produced from Polypedilum vanderplanki larvae in a desiccated state, a P. vanderplanki EST database was constructed, and genes encoding LEA proteins were isolated. This resulted in the successful isolation of three types of novel gene encoding LEA-like proteins. When secondary structure predictions and motif searches were performed on the proteins deduced from each of the genes, all three proteins had α-helix-rich structures and LEA_4 motifs, which are characteristic of LEA proteins. Moreover, the recombinant proteins synthesized from these genes were heat soluble even when boiling, so that these proteins have hydrophilic properties as high as plant LEA proteins. Therefore, the three isolated genes were found to be novel P. vanderplanki-derived LEA genes. Furthermore, introduction of these genes into animal cells successfully conferred desiccation resistance to the cells.
摘要翻译： 本发明的目的是提供编码昆虫耐干燥性蛋白质的多核苷酸及其用途。 在干燥状态下从Polypedilum vanderplanki幼虫生产cDNA文库，构建了P.vanderplanki EST数据库，分离了编码LEA蛋白的基因。 这导致了三种类型的编码LEA样蛋白的新基因的成功分离。 对从每个基因推导的蛋白质进行二级结构预测和基序搜索时，所有三种蛋白质都具有α-螺旋结构和LEA_4基序，这是LEA蛋白质的特征。 此外，由这些基因合成的重组蛋白即使在沸腾时也是热溶性的，因此这些蛋白质具有与植物LEA蛋白一样高的亲水性。 因此，发现三个分离的基因是新的P. vanderplanki衍生的LEA基因。 此外，将这些基因导入动物细胞成功地赋予细胞干燥抗性。
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