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公开(公告)号：US20110209251A1
公开(公告)日：2011-08-25
申请号：US13099917
申请日：2011-05-03
申请人： Yukoh HIEI , Keisuke Kasaoka , Yuji Ishida
发明人： Yukoh HIEI , Keisuke Kasaoka , Yuji Ishida
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8207
摘要： A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.
摘要翻译： 公开了一种用于基因导入的方法，其基因导入的效率比常规农杆菌法更高，而且不会损伤组织。 根据本发明的方法，通过伴随的植物细胞或植物组织的离心来促进由属于农杆菌属的细菌引入植物细胞的基因的效率。
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公开(公告)号：US20110030100A1
公开(公告)日：2011-02-03
申请号：US12902631
申请日：2010-10-12
申请人： Yukoh HIEI , Keisuke Kasaoka , Yuji Ishida
发明人： Yukoh HIEI , Keisuke Kasaoka , Yuji Ishida
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8207
摘要： A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.
摘要翻译： 公开了一种用于基因导入的方法，其基因导入的效率比常规农杆菌法更高，而且不会损伤组织。 根据本发明的方法，通过伴随的植物细胞或植物组织的离心来促进由属于农杆菌属的细菌引入植物细胞的基因的效率。
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3.发明授权Recombinant vector, method for giving immunity against PVY-T to potato plant, and potato plant having immunity against PVY-T 失效重组载体，对PVY-T免疫马铃薯植物的方法和具有抗PVY-T免疫力的马铃薯植株公开(公告)号：US06228637B1
公开(公告)日：2001-05-08
申请号：US08558935
申请日：1995-11-13
IPC分类号： C12N1500
CPC分类号： C07K14/005 , A01H5/04 , C12N15/8283 , C12N2770/40022
摘要： A recombinant vector which can transform potato plants so as to confer upon the potato plant immunity against potato virus Y necrosis line is disclosed. The recombinant vector according to the present invention comprises a promoter which functions in a potato plant cell; an operably linked leader sequence of RNA4 of cucumber mosaic virus, which is located downstream of the promoter; and an operably linked sequence which encodes a coat protein of potato virus Y necrosis line, which is located downstream of said leader sequence; and the recombinant vector is capable of transforming potato plants.
摘要翻译： 公开了能够转化马铃薯植物以赋予马铃薯植株免疫马铃薯病毒Y坏死系的重组载体。 根据本发明的重组载体包含在马铃薯植物细胞中起作用的启动子; 位于启动子下游的黄瓜花叶病毒RNA4的可操作连接的前导序列; 和可操作地连接的序列，其编码位于所述前导序列下游的马铃薯病毒Y坏死系的外壳蛋白; 重组载体能够转化马铃薯植物。
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公开(公告)号：US6084089A
公开(公告)日：2000-07-04
申请号：US894731
申请日：1997-10-27
申请人： Toshiki Mine , Akio Ohyama , Toru Hiyoshi , Keisuke Kasaoka
发明人： Toshiki Mine , Akio Ohyama , Toru Hiyoshi , Keisuke Kasaoka
CPC分类号： C07K14/415 , C12N15/8226 , C12N15/8237
摘要： This invention discloses a novel cold-inducible promoter which induces gene expression at low temperatures in potato tubers but which is scarcely induced in organs other than tuber or at normal temperature, which induces gene expression for a long time not less than five months. The promoters of this invention are the DNA sequence having a nucleotide sequence from first to 3546th nucleotide in the nucleotide sequence shown in SEQ ID NO: 1, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity and the DNA sequence having a nucleotide sequence from first to 4120th nucleotide in the nucleotide sequence shown in SEQ ID NO: 2, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as the said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity.
摘要翻译： PCT No.PCT / JP96 / 03822 Sec。 371日期1997年10月27日第 102（e）日期1997年10月27日PCT 1996年12月26日PCT公布。 第WO97 / 24449号公报 日本1997年7月10日本发明公开了一种在马铃薯块茎中诱导低温基因表达的新型冷诱导型启动子，但在块茎以外的器官或常温下几乎不诱导基因表达，其诱导基因表达长时间不小于 五个月。 本发明的启动子是具有SEQ ID NO：1所示核苷酸序列的第一至第3546位核苷酸序列的DNA序列或其具有冷诱导型启动子活性的部分的DNA序列，或具有相同核苷酸的DNA序列 序列作为所述DNA序列，除了一个或多个核苷酸被缺失或取代，或插入或添加一个或多个核苷酸，哪个DNA序列具有冷诱导启动子活性，并且DNA序列具有从第一至第4120位核苷酸的核苷酸序列 SEQ ID NO：2所示的核苷酸序列或其具有冷诱导型启动子活性的部分，或具有与所述DNA序列相同的核苷酸序列的DNA序列，除了一个或多个核苷酸被缺失或取代，或一个 或更多个核苷酸插入或添加，哪个DNA序列具有冷诱导型启动子活性。
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5.发明授权Method of improving gene transfer efficiency into plant cells utilizing heat and centrifugation 有权使用热和离心法将基因转移效率提高到植物细胞的方法公开(公告)号：US07902426B1
公开(公告)日：2011-03-08
申请号：US10089695
申请日：2000-08-03
申请人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
发明人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
IPC分类号： C12N15/82
CPC分类号： C12N15/8201 , C12N15/8205 , C12N15/8206
摘要： A method for gene transfer by which higher efficiency for gene transfer than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene transfer into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying heat treatment and centrifugation treatment of the plant cells or plant tissue.
摘要翻译： 公开了一种用于基因转移的方法，其通过简单地实现基因转移的效率高于常规农杆菌法，而不损伤组织。 根据本发明的方法，通过伴随对植物细胞或植物组织的热处理和离心处理，促进了属于农杆菌属的细菌的基因转移到植物细胞中的效率。
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公开(公告)号：US20050132440A1
公开(公告)日：2005-06-16
申请号：US10992670
申请日：2004-11-22
申请人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
发明人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8283
摘要： A gene silencing vector according to the present invention comprises a vector which includes a promoter, an enhancer sequence in the downstream of the promoter, and a gene encoding a potyvirus-origin coat protein in the downstream of the enhancer sequence, wherein in order to cause gene silencing of the specific target gene in a host plant, the vector is used with a specific target gene or a gene that is homologous to the target gene inserted in the vicinity of the gene encoding the coat protein.
摘要翻译： 根据本发明的基因沉默载体包含载体，其包含启动子，启动子下游的增强子序列和编码加强子序列下游的病毒源外壳蛋白的基因，其中为了引起 在宿主植物中特异性靶基因的基因沉默，该载体与特异性靶基因或与插入编码外壳蛋白的基因附近的靶基因同源的基因使用。
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公开(公告)号：US09840714B2
公开(公告)日：2017-12-12
申请号：US13099917
申请日：2011-05-03
申请人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
发明人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8207
摘要： A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.
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公开(公告)号：US07960611B2
公开(公告)日：2011-06-14
申请号：US12902631
申请日：2010-10-12
申请人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
发明人： Yukoh Hiei , Keisuke Kasaoka , Yuji Ishida
IPC分类号： C12N15/82
CPC分类号： C12N15/8205 , C12N15/8201 , C12N15/8207
摘要： A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene introduction into plant cells by a bacterium belonging to genus Agrobacterium is promoted by accompanying centrifugation of the plant cells or plant tissue.
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公开(公告)号：US20080044897A1
公开(公告)日：2008-02-21
申请号：US11874829
申请日：2007-10-18
申请人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
发明人： Keisuke Kasaoka , Yasuhito Saito , Shigeru Kuwata
IPC分类号： C12N15/00
CPC分类号： C12N15/8216 , C12N15/8218 , C12N15/8283
摘要： A gene silencing vector according to the present invention comprises a vector which includes a promoter, an enhancer sequence in the downstream of the promoter, and a gene encoding a potyvirus-origin coat protein in the downstream of the enhancer sequence, wherein in order to cause gene silencing of the specific target gene in a host plant, the vector is used with a specific target gene or a gene that is homologous to the target gene inserted in the vicinity of the gene encoding the coat protein.
摘要翻译： 根据本发明的基因沉默载体包含载体，其包含启动子，启动子下游的增强子序列和编码加强子序列下游的病毒源外壳蛋白的基因，其中为了引起 在宿主植物中特异性靶基因的基因沉默，该载体与特异性靶基因或与插入编码外壳蛋白的基因附近的靶基因同源的基因使用。
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10.发明授权DNA encoding ATP-dependent fructose 6-phosphate 1-phosphotransferase originating from plant, recombinant vector containing the same and method for changing sugar content in plant cells under low temperature 失效编码源自植物的ATP依赖性果糖6-磷酸1-磷酸转移酶的DNA，含有相同的重组载体和在低温下改变植物细胞中的糖含量的方法
公开(公告)号：US5824862A
公开(公告)日：1998-10-20
申请号：US416870
申请日：1995-06-09
CPC分类号： C12N9/1205 , C12N15/8245 , C12N9/12
摘要： A DNA encoding cold stable PFK, a recombinant vector which can express a cold stable PFK in a host cell, and a method for changing sugar content in plant cells under low temperature using the recombinant vector are disclosed. The present invention provides a DNA encoding ATP dependent fructose 6-phosphate 1-phosphotransferase originating from a plant, as well as a recombinant vector comprising the DNA and a plant transformed with the DNA.
摘要翻译： PCT No.PCT / JP94 / 01352 Sec。 371日期：1995年6月9日 102（e）1995年6月9日PCT PCT 1994年8月16日PCT公布。 公开号WO95 / 05457 日期：1995年2月23日公开了一种编码冷稳定性PFK的DNA，其是能够在宿主细胞中表达冷稳定性PFK的重组载体，以及使用重组载体在低温下改变植物细胞中的糖含量的方法。 本发明提供了编码源自植物的ATP依赖性果糖-6-磷酸1-磷酸转移酶的DNA，以及包含DNA和用该DNA转化的植物的重组载体。
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