公开(公告)号：US20180071730A1

公开(公告)日：2018-03-15

申请号：US15634097

申请日：2017-06-27

申请人： Keith Joseph Breinlinger ,  Eric D. Hobbs ,  Dorian Liepmann ,  Joshua Tanner Nevill ,  Mark P. White ,  Maria Jimena Loureiro

发明人： Keith Joseph Breinlinger ,  Eric D. Hobbs ,  Dorian Liepmann ,  Joshua Tanner Nevill ,  Mark P. White ,  Maria Jimena Loureiro

IPC分类号： B01L3/00 ,  C12M3/06

CPC分类号： B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/086 ,  C12M23/16 ,  Y10T436/2575

摘要： Some configurations of a microfluidic apparatus can comprise a fluidic circuit of interconnected fluidic structures into which a plurality of different media can be introduced or extracted. A variety of operations can be performed with the different media including isolating with a second medium one or more of the fluidic structures that is filled partially or fully with a first medium. Discrete volumes of a medium can be moved through the isolating second medium to deliver materials or micro-objects to or remove micro-objects or materials from a fluidic structure that is otherwise isolated by the second medium. Some configurations of a microfluidic apparatuses can isolate microfluidic structures in a microfluidic apparatus using flow rates or blocking structures, and some configurations can manage bubbles in fluidic structures.