公开(公告)号：US09329169B2

公开(公告)日：2016-05-03

申请号：US12734102

申请日：2008-10-29

申请人： Robert Lance Wells ,  Ronald C. Orlando ,  Stephen Dalton ,  Kelley W. Moremen ,  James Michael Pierce ,  James A. Atwood, III ,  Michael Tiemeyer ,  William S. York

发明人： Robert Lance Wells ,  Ronald C. Orlando ,  Stephen Dalton ,  Kelley W. Moremen ,  James Michael Pierce ,  James A. Atwood, III ,  Michael Tiemeyer ,  William S. York

IPC分类号： C12Q1/02 ,  G01N33/50 ,  G01N33/68

CPC分类号： G01N33/502 ,  G01N33/6848 ,  G01N2400/00 ,  G01N2400/12 ,  G01N2400/38 ,  G01N2458/15

摘要： The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, differentiation conditions, disease and/or therapeutic progression, diagnosing disease states, determining drug activity, establishing manufacturing efficiencies and for determining the half-life of glycans in cells.

摘要翻译： 本发明涉及同位素标记聚糖并促进生物细胞的糖组成的高通量定量/比较分析方法。 该方法特别适用于鉴定分化细胞及其糖基特征，分化条件，疾病和/或治疗进展，诊断疾病状态，确定药物活性，建立制造效率和确定细胞中聚糖的半衰期。

公开(公告)号：US06524820B1

公开(公告)日：2003-02-25

申请号：US09709677

申请日：2000-11-09

申请人： J. Michael Pierce ,  Kelley W. Moremen ,  Jin Kyu Lee

发明人： J. Michael Pierce ,  Kelley W. Moremen ,  Jin Kyu Lee

IPC分类号： C12N121

CPC分类号： C07K14/4726 ,  C07K14/463 ,  C07K14/71

摘要： The present disclosure provides a novel family of C-type lectins from vertebrates. As specifically exemplified, this family of lectins includes a melibiose-specific lectin (XL35) from Xenopus laevis oocytes, and two human lectins, HL-3 and HL-13, specifically expressed in endothelial cells such as blood vessels, stomach, small intestine, heart and tissue and in small intestine, respectively. Amino acid sequences of the lectin proteins are provided herein, together with the nucleotide sequences encoding these proteins.

摘要翻译： 本公开提供了来自脊椎动物的新型C型凝集素家族。 如具体示例的那样，这种凝集素家族包括来自非洲爪蟾卵母细胞的蜜二糖特异性凝集素（XL35）和两种人类凝集素HL-3和HL-13，特异性表达在内皮细胞如血管，胃，小肠， 心脏和组织和小肠。 本文提供了凝集素蛋白的氨基酸序列以及编码这些蛋白质的核苷酸序列。

公开(公告)号：US06204431B1

公开(公告)日：2001-03-20

申请号：US08925834

申请日：1997-09-05

申请人： Pedro A. Prieto ,  John J. Kopchick ,  Richard D. Cummings ,  James M. Pierce ,  David F. Smith ,  Kelley W. Moremen

发明人： Pedro A. Prieto ,  John J. Kopchick ,  Richard D. Cummings ,  James M. Pierce ,  David F. Smith ,  Kelley W. Moremen

IPC分类号： A01K67027

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K2217/05 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2227/107 ,  A01K2267/01 ,  A23C9/20 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/1077

摘要： The subject invention relates to methods of producing non-human transgenic mammals which produce various oligosaccharides and glycoconjugates in their milk. Additionally, the subject invention relates to the mammals themselves, the milk which they produce, compositions comprising the milk, fractions of the milk, and the purified oligosaccharides, as well as glyconjugates, present in the milk.

摘要翻译： 本发明涉及生产在其乳中产生各种寡糖和糖缀合物的非人转基因哺乳动物的方法。 此外，本发明涉及哺乳动物本身，它们产生的乳，包含乳的组分，乳的部分和纯化的寡糖以及存在于牛奶中的糖缀合物。

公开(公告)号：US6146849A

公开(公告)日：2000-11-14

申请号：US124758

申请日：1998-06-04

申请人： J. Michael Pierce ,  Kelley W. Moremen ,  Jin Kyu Lee

发明人： J. Michael Pierce ,  Kelley W. Moremen ,  Jin Kyu Lee

IPC分类号： C07K14/46 ,  C07K14/47 ,  C07K14/71 ,  C12N1/21 ,  C12N15/12 ,  C12N5/10 ,  C12N15/63

CPC分类号： C07K14/4726 ,  C07K14/463 ,  C07K14/71

摘要： The present disclosure provides a novel family of C-type lectins from vertebrates. As specifically exemplified, this family of lectins includes a melibiose-specific lectin (XL35) from Xenopus laevis oocytes, and two human lectins, HL-3 and HL-13, specifically expressed in endothelial cells such as blood vessels, stomach, small intestine, heart and tissue and in small intestine, respectively. Amino acid sequences of the lectin proteins are provided herein, together with the nucleotide sequences encoding these proteins.