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    Oligosaccharides and glycoproteins produced in milk of transgenic non-human mammals
    6.
    发明授权
    Oligosaccharides and glycoproteins produced in milk of transgenic non-human mammals 失效
    在转基因非人哺乳动物的乳中产生的寡糖和糖蛋白

    公开(公告)号：US5891698A

    公开(公告)日：1999-04-06

    申请号：US433271

    申请日：1995-05-02

    申请人： Pedro Antonio Prieto ,  David Fletcher Smith ,  Richard Dale Cummings ,  John Joseph Kopchick ,  Pradip Mukerji ,  Kelley Wilson Moremen ,  James Michael Pierce

    发明人： Pedro Antonio Prieto ,  David Fletcher Smith ,  Richard Dale Cummings ,  John Joseph Kopchick ,  Pradip Mukerji ,  Kelley Wilson Moremen ,  James Michael Pierce

    IPC分类号： A01K67/027 ,  A23C9/20 ,  C12N9/10 ,  C12N15/09 ,  C12N15/85 ,  C12P21/06 ,  C12N5/00 ,  C12N15/00

    CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A23C9/20 ,  C12N9/1048 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/10 ,  A01K2227/105 ,  A01K2267/01

    摘要： The invention relates to humanized milk. The milk is produced by a non-human transgenic mammal wherein the genome of said transgenic non-human mammal contains at least one heterologous gene encoding for a human catalytic entity and wherein the catalytic entity produces oligosaccharides and glycoconjugates that are present in the milk of said transgenic non-human mammal. An especially useful catalytic entity is human glycosyltransferases which produce oligosaccharides and glyconjugates. Specifically exemplified, is the production of 2'-fucosyl-lactose in the milk of transgenic mice which contain and express a transgene encoding .alpha.-1,2-fucosyltransferase operatively linked to a mammary gland specific promoter. A method of obtaining humanized milk is disclosed. The method comprises the steps of (a) inserting into the genome of a non-human mammal a heterologous gene encoding the production of a human catalytic entity wherein said catalytic entity produces a secondary gene product in the milk of said non-human mammal; and (b) milking said non-human mammal. The humanized milk may be used in the preparation of an enteral nutritional product useful in the nutritive maintenance of an animal.

    摘要翻译： 本发明涉及人造牛奶。 所述乳由非人转基因哺乳动物产生，其中所述转基因非人哺乳动物的基因组含有至少一个编码人类催化剂实体的异源基因，并且其中所述催化剂实体产生存在于所述第二种哺乳动物乳的乳中的寡糖和糖缀合物 转基因非人哺乳动物。 特别有用的催化剂实体是产生寡糖和glyconjugates的人类糖基转移酶。 具体举例说明的是在转基因小鼠的乳中产生2'-岩藻糖基 - 乳糖，其含有和表达编码与乳腺特异性启动子有效连接的α-1,2-岩藻糖基转移酶的转基因。 公开了获得人性化牛奶的方法。 该方法包括以下步骤：（a）在非人哺乳动物的基因组中插入编码人催化剂实体的异源基因，其中所述催化剂实体在所述非人哺乳动物的乳中产生次级基因产物; 和（b）挤压所述非人哺乳动物。 人造牛奶可用于制备可用于动物营养维持的肠内营养产品。