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公开(公告)号:US20070269870A1
公开(公告)日:2007-11-22
申请号:US11254250
申请日:2005-10-18
申请人: George Church , Noubar Afeyan , Joseph Jacobson , Brian Baynes , Kenneth Nesmith , Brad Chapman , Bettina Strack-Logue
发明人: George Church , Noubar Afeyan , Joseph Jacobson , Brian Baynes , Kenneth Nesmith , Brad Chapman , Bettina Strack-Logue
IPC分类号: C12P19/34
CPC分类号: C12N15/66 , C12N15/10 , C12Q1/6844 , C12Q2531/113 , C12Q2521/514 , C12Q2533/107 , C12Q2521/307 , C12Q2533/101
摘要: Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of nucleic acid polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or nucleic acid products, and sequence optimization and oligonucleotides design. Also provided are low-purity arrays of nucleic acids and methods for assembling nucleic acids using oligonucleotides obtained from low-purity arrays.
摘要翻译: 公开了制造合成DNA的方法,即通过核酸聚合物的化学合成至少大部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中,所述方法包括使用“通用”引物预先扩增一种或多种寡核苷酸,降低寡核苷酸和/或核酸产物中的错误率,以及序列优化和寡核苷酸设计。 还提供了低纯度核酸阵列和使用从低纯度阵列获得的寡核苷酸组装核酸的方法。