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    Methods for assembly of high fidelity synthetic polynucleotides
    2.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20070122817A1

    公开(公告)日：2007-05-31

    申请号：US11067812

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6806 ,  C12Q1/6844 ,  C12Q2537/113 ,  C12Q2527/107 ,  C12Q2521/501

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。

    Methods for assembly of high fidelity synthetic polynucleotides
    3.
    发明申请
    Methods for assembly of high fidelity synthetic polynucleotides 审中-公开
    装配高保真合成多核苷酸的方法

    公开(公告)号：US20060194214A1

    公开(公告)日：2006-08-31

    申请号：US11068321

    申请日：2005-02-28

    申请人： George Church ,  Joseph Jacobson ,  Brian Baynes

    发明人： George Church ,  Joseph Jacobson ,  Brian Baynes

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6813 ,  C12Q2523/305 ,  C12Q2525/131 ,  C12Q2525/155

    摘要： Disclosed are methods of manufacturing synthetic DNAs, that is, DNAs made at least in significant part by chemical synthesis of polynucleotide polymers. Also provided are methods for assembling plural DNAs in the same pool by multiplexed assembly of synthetic oligonucleotides. In exemplary embodiments, the methods involve pre-amplification of one or more oligonucleotides using “universal” primers, reduction of the error rate in oligonucleotide and/or polynucleotide products, and sequence optimization and oligonucleotides design.

    摘要翻译： 公开了制造合成DNA的方法，即通过化学合成多核苷酸聚合物至少部分制备的DNA。 还提供了通过合成寡核苷酸的多重组装在同一池中组装多个DNA的方法。 在示例性实施方案中，所述方法包括使用“通用”引物预扩增一个或多个寡核苷酸，降低寡核苷酸和/或多核苷酸产物中的错误率，以及序列优化和寡核苷酸设计。

    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell
    4.
    发明申请
    Compositions and Methods for the Regulation of Multiple Genes of Interest in a Cell 审中-公开
    用于调节细胞中多种感兴趣基因的组合物和方法

    公开(公告)号：US20120315670A1

    公开(公告)日：2012-12-13

    申请号：US13505376

    申请日：2010-11-02

    申请人： Joseph Jacobson ,  George Church

    发明人： Joseph Jacobson ,  George Church

    IPC分类号： C12P21/00 ,  C12N15/63

    CPC分类号： C12N15/1051 ,  C12N15/63

    摘要： Methods and compositions are provided for manipulating the genome of host cell to produce at least one exogenous gene product. Also provided are methods and composition for producing a programmable cell comprising a plurality of exogenous genes, wherein each exogenous gene is under the control of a disrupted regulatory sequence and wherein the disrupted regulatory sequences are restored by in vivo recombination. Preferably, the gene of interest is under the control of a genetically altered promoter which sequence recombination effects the expression of the exogenous gene(s).

    摘要翻译： 提供了用于操纵宿主细胞基因组以产生至少一种外源基因产物的方法和组合物。 还提供了用于生产包含多个外源基因的可编程细胞的方法和组合物，其中每个外源基因处于破坏的调节序列的控制下，并且其中破坏的调节序列通过体内重组恢复。 优选地，感兴趣的基因在遗传改变的启动子的控制下，该序列重组影响外源基因的表达。

    Methods and devices for nucleic acid synthesis
    7.
    发明授权
    Methods and devices for nucleic acid synthesis 有权
    用于核酸合成的方法和装置

    公开(公告)号：US08716467B2

    公开(公告)日：2014-05-06

    申请号：US13038714

    申请日：2011-03-02

    申请人： Joseph Jacobson

    发明人： Joseph Jacobson

    IPC分类号： C07H21/00

    CPC分类号： C12P19/34 ,  C12M1/00 ,  C12N15/1068

    摘要： Disclosed are devices and methods to synthesize polynucleotides and libraries of polynucleotides such as libraries of oligonucleotides. In exemplary embodiments, the device includes a support having a plurality of features. Each feature contains a plurality of oligonucleotides. Within each feature, each of the plurality of oligonucleotides includes an identical predetermined subunit sequence of X nucleosides and a degenerate sequence of Y nucleosides. A predetermined combination of a subset of the features can be used to produce a polynucleotide having a predetermined sequence of Z nucleosides.

    摘要翻译： 公开了用于合成寡核苷酸文库的多核苷酸和多核苷酸文库的装置和方法。 在示例性实施例中，该装置包括具有多个特征的支撑件。 每个特征包含多个寡核苷酸。 在每个特征中，多个寡核苷酸中的每一个包括X核苷的相同的预定亚基序列和Y核苷的简并序列。 可以使用特征子集的预定组合来产生具有预定的Z核苷序列的多核苷酸。

    Methods and Devices for In Situ Nucleic Acid Synthesis
    8.
    发明申请
    Methods and Devices for In Situ Nucleic Acid Synthesis 有权
    用于原位核酸合成的方法和装置

    公开(公告)号：US20130309725A1

    公开(公告)日：2013-11-21

    申请号：US13700291

    申请日：2011-05-26

    申请人： Joseph Jacobson ,  Senthil Ramu ,  Daniel Schindler

    发明人： Joseph Jacobson ,  Senthil Ramu ,  Daniel Schindler

    IPC分类号： C12P19/34

    CPC分类号： C12N15/1068 ,  C12N15/1093 ,  C12N15/66 ,  C12P19/34

    摘要： Disclosed are compositions, methods and devices for the in situ synthesis of nucleic acids. In an exemplary embodiment, a support-bound oligonucleotide is elongated by addition of one or more nucleotides by hybridization of a partially double-stranded oligonucleotide, ligation and removal of unwanted nucleotides.

    摘要翻译： 公开了用于原位合成核酸的组合物，方法和装置。 在一个示例性实施方案中，通过部分双链寡核苷酸的杂交，连接和去除不需要的核苷酸，加入一个或多个核苷酸来延伸载体结合的寡核苷酸。