公开(公告)号：US20080241866A1

公开(公告)日：2008-10-02

申请号：US12079944

申请日：2008-03-27

Applicant: Jonas Korlach ,  Kevan Samiee ,  Stephen W. Turner ,  Jake Freudenthal

Inventor： Jonas Korlach ,  Kevan Samiee ,  Stephen W. Turner ,  Jake Freudenthal

IPC: C12Q1/66 ,  C12Q1/00 ,  C12Q1/48 ,  C12Q1/37 ,  C07H21/00 ,  G01N21/64 ,  C12M1/00 ,  C12Q1/34 ,  C12Q1/42

CPC classification number: G01N21/6452 ,  G01N21/648

Abstract: Composition, systems, apparatus and methods of enhancing fluorescent signals in biochemical are described. Metal particle proximity to enzymes that produce fluorescent products provide enhanced fluorescence of the product and plasmon resonance of the metal particle. Multi-labeled nucleotides enhance signal production. Reflectance of illumination light and emitted fluorescence increase signal strength for a given illumination light.

Abstract translation: 描述了增强生物化学荧光信号的组成，系统，装置和方法。 与产生荧光产物的酶接近的金属颗粒提供产物的增强的荧光和金属颗粒的等离子体共振。 多标记核苷酸增强信号产生。 照明光的反射和发出的荧光增加给定照明光的信号强度。

公开(公告)号：US20070020779A1

公开(公告)日：2007-01-25

申请号：US11280941

申请日：2005-11-16

Applicant: Samuel Stavis ,  Joshua Edel ,  Kevan Samiee ,  Harold Craighead

Inventor： Samuel Stavis ,  Joshua Edel ,  Kevan Samiee ,  Harold Craighead

IPC: H01L21/66

CPC classification number: A61K49/0067 ,  A61K49/0056 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y15/00 ,  B82Y20/00 ,  B82Y30/00 ,  G01N33/588 ,  Y10S438/962 ,  Y10S977/70 ,  Y10S977/774

Abstract: A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

Abstract translation: 使用在约500nm正方形横截面的熔融二氧化硅中制造的纳米流体通道来分离，检测和识别各种量子点缀合物。 该通道可以快速检测溶液中的每个荧光实体。 使用选定波长的激光来激发多种量子点和有机分子，并且发射光谱被解析而没有显着的信号抑制。 然后将量子点与有机分子缀合并检测以证明有效的多色检测。 PCH用于分析重合检测并表征结合程度。 使用小的流体通道检测量子点作为荧光标记被证明是多重单分子研究的有效技术。 单分子结合事件的检测具有多种应用，包括高通量免疫测定。