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公开(公告)号:US09290735B2
公开(公告)日:2016-03-22
申请号:US12866588
申请日:2009-02-05
申请人: Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
发明人: Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
CPC分类号: C12N5/0631 , C12M23/12 , C12M23/16 , C12M23/22 , C12N5/0062 , C12N2535/10
摘要: To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.
摘要翻译: 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞,并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时,可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器,其具有表面上的多个这种微量容器。
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公开(公告)号:US20100331216A1
公开(公告)日:2010-12-30
申请号:US12866588
申请日:2009-02-05
申请人: Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
发明人: Masahiro Sokabe , Kishio Furuya , Go Tazaki , Hitoshi Tsuruta , Motohiro Fukuda
IPC分类号: C40B50/06
CPC分类号: C12N5/0631 , C12M23/12 , C12M23/16 , C12M23/22 , C12N5/0062 , C12N2535/10
摘要: To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.
摘要翻译: 提供能够通过抑制去分化来长时间维持体内功能的细胞培养方法。 根据本发明的细胞培养方法是在微小分隔空间中以多层状态培养细胞,并获得具有类似于体内功能的功能的组织结构。 当细胞是乳腺上皮细胞时,可以形成中空的类固醇样结构。 微细分隔空间特别优选为细胞培养容器中的微型容器,其具有表面上的多个这种微量容器。
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