公开(公告)号：US20240307554A1

公开(公告)日：2024-09-19

申请号：US18576991

申请日：2022-07-07

申请人： NTRANS TECHNOLOGIES BV ,  Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Marieke Visscher ,  Anna Chatsisvili ,  Axel Beier ,  Niels Geijsen

IPC分类号： A61K48/00 ,  A61K38/46 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC分类号： A61K48/0033 ,  A61K38/465 ,  C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12N2310/20

摘要： The invention relates to transduction buffers and methods for transducing molecules into cells using said buffers. The invention also relates to pharmaceutical compositions comprising said transduction buffers and methods of treatment involving the use of said buffers or pharmaceutical compositions to treat diseases, in particular genetic diseases.

公开(公告)号：US20230287395A1

公开(公告)日：2023-09-14

申请号：US18020391

申请日：2021-08-13

申请人： CAMBRIDGE ENTERPRISE LIMITED ,  KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN

发明人： Florian Hollfelder ,  Tomasz Kaminski ,  Joachim De Jonghe ,  Fredrik Salmen ,  Alexander Van Oudenaarden

IPC分类号： C12N15/10 ,  B01L3/00

CPC分类号： C12N15/1065 ,  B01L3/502761 ,  C12N15/1096 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2400/0487

摘要： Modular microfluidic devices, systems and methods for total RNA analyses The invention relates to microfluidic methods of preparing a sequencing library for analyses of total RNA. The invention also relates to modular microfluidic systems for carrying out these methods.

公开(公告)号：US20230170042A1

公开(公告)日：2023-06-01

申请号：US17919970

申请日：2021-04-23

申请人： KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN

发明人： Wouter Leonard De Laat ,  Amin Allahyar ,  Erik Cornelis Splinter

IPC分类号： G16B20/00 ,  C12Q1/6827

CPC分类号： G16B20/00 ,  C12Q1/6827

摘要： The present invention relates to the field of molecular biology and more in particular to DNA technology. The invention relates to strategies for assessing structural integrity of DNA sequences of a genomic region of interest, which has clinical applications in diagnostics and personalized cancer therapy. In particular, the invention provides a method of detecting a chromosomal rearrangement involving a genomic region of interest.

公开(公告)号：US20220340879A1

公开(公告)日：2022-10-27

申请号：US17611561

申请日：2020-05-18

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Willibrordus Barend Maria De Lau ,  Johannes Carolus Clevers

IPC分类号： C12N5/071

摘要： The invention relates to improved methods for culturing an epithelial stem cell or an organoid comprising epithelial stem cells. The invention also relates to culture media suitable for use with said methods, organoids obtainable or obtained by said methods and uses of said culture methods, media and organoids in drug discovery and validation, toxicity assays, diagnostics and therapy.

公开(公告)号：US20220088052A1

公开(公告)日：2022-03-24

申请号：US17424505

申请日：2020-01-22

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Eva van Rooij ,  Arwa Obada Abdelrahman Reda Kohela

IPC分类号： A61K31/713 ,  A61K31/165 ,  A61K31/055 ,  A61K31/609

摘要： The present invention relates to novel treatments for treating cardiac disease involving fibro-fatty replacement, such as arrhythmogenic cardiomyopathy, atrial fibrillation, myocardial infarction and dilated cardiomyopathy. Such cardiac diseases can e.g. be caused by a mutation in a desmosomal protein such as plakophilin-2 (PKP2). The invention provides for agents for use in the prevention or treatment of such cardiac diseases, wherein the agent is at least one of: a) an agent that causes a reduction in expression in at least one TFAP2 subtype; and, b) an agent that causes a reduction in TFAP2-induced transcription. More preferably the agent is at least one of: a) an inhibitor of TFAP2; and, b) an agent that causes an increase in expression of PKP2.

公开(公告)号：US11035852B2

公开(公告)日：2021-06-15

申请号：US16003293

申请日：2018-06-08

申请人： UMC Utrecht Holding B.V. ,  Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Jeffrey Matthijn Beekman ,  Johanna Florentia Dekkers ,  Cornelis Korstiaan Van Der Ent ,  Johannes C. Clevers

IPC分类号： G01N33/50

摘要： The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.

公开(公告)号：US11034935B2

公开(公告)日：2021-06-15

申请号：US15665363

申请日：2017-07-31

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Meritxell Huch Ortega ,  Johannes Carolus Clevers

IPC分类号： C12N5/071 ,  A61K35/407

摘要： The invention relates to a liver organoid, uses thereof and method for obtaining them.

公开(公告)号：US10940180B2

公开(公告)日：2021-03-09

申请号：US15813863

申请日：2017-11-15

申请人： Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Johannes C. Clevers ,  Nicholas Barker ,  Andrea Haegebarth ,  Marcus Lambertus Van de Wetering

IPC分类号： G01N33/50 ,  A61K38/17 ,  C12N5/071 ,  A61K45/06

摘要： The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue.
In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound.
The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.

公开(公告)号：US20210040454A1

公开(公告)日：2021-02-11

申请号：US16078354

申请日：2017-03-01

申请人： Koninklijke Nederlandse Akademie Van Wetenschappen

发明人： Johannes Carolus Clevers ,  Helmuth Gehart

IPC分类号： C12N5/071 ,  G01N33/50 ,  A61K35/407

摘要： The invention relates to differentiation methods for progenitor cells, e.g. mammalian epithelial stem cells, differentiation media for use in said methods, organoids and cells obtainable by said methods and uses, including therapeutic uses, thereof.

公开(公告)号：US20200045978A1

公开(公告)日：2020-02-13

申请号：US16341734

申请日：2017-10-23

申请人： DCM De Ceuster Meststoffen NV ,  Nederlands Instituut voor Ecologie van de Koninklijke Nederlandse Akademie van Wetenschappen

发明人： Viviane Cordovez da Cunha ,  Inge Hanssen ,  Tom Jozef Justine De Ceuster ,  Victor Jose Carrion-Bravo ,  Josephus Maria Raaijmakers

IPC分类号： A01N63/02 ,  A01C1/06

摘要： Use of a bacterial composition comprising at least one plant growth-promoting bacterium from the Microbacterium genus, for producing biostimulant effects in seeds, seedlings and/or plants, comprising the step of priming said seeds, or seedlings or plants. The bacterial composition particularly comprises Microbacterium strains EC8 and/or all strains sharing >98% nucleotide sequence homology with EC8 at the 16S rRNA gene level (in particular strains EC19 and W283), and/or strains EC6, W211, W236, W219 and W269. The bacterial composition is particularly applied to a plant growth medium, for instance before seedling transplantation. Very good biostimulant effects have been obtained for lettuce, tomato and basil plants.