公开(公告)号：US09290537B2

公开(公告)日：2016-03-22

申请号：US13996583

申请日：2011-12-23

Applicant: Annemieke Madder ,  Kurt Hoogewijs ,  Annelies Deceuninck ,  Lieselot Carrette

Inventor： Annemieke Madder ,  Kurt Hoogewijs ,  Annelies Deceuninck ,  Lieselot Carrette

IPC: A61K38/00 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  C07K1/107 ,  C07K1/00 ,  C07K1/13

CPC classification number: C07K1/113 ,  C07K1/006 ,  C07K1/1075 ,  C07K1/1077 ,  C07K1/13 ,  C12N9/0051 ,  C12Y108/01009

Abstract: The present invention relates to a method for cross-linking peptides using an activated furan-moiety. In particular, the present invention provides a method for cross-linking peptides comprising the steps of: a) providing a composition comprising furan-peptides, said furan-peptides comprising at least one amino acid comprising a furan-moiety; b) contacting said composition comprising furan-peptides with second peptides, thereby obtaining a mixture comprising furan-peptides and second peptides; c) adding an activation signal to said mixture of step b), thereby activating said furan-peptides to activated furan-peptides, and d) reacting said activated furan-peptides with said second peptides, thereby cross-linking said activated furan-peptides with said second peptides.

Abstract translation: 本发明涉及使用活化的呋喃部分交联肽的方法。 特别地，本发明提供了交联肽的方法，包括以下步骤：a）提供包含呋喃肽的组合物，所述呋喃肽包含至少一个包含呋喃部分的氨基酸; b）使包含呋喃肽的所述组合物与第二肽接触，从而获得包含呋喃肽和第二肽的混合物; c）向步骤b）的所述混合物中加入活化信号，从而将所述呋喃肽激活成活化的呋喃肽，和d）使所述活化的呋喃肽与所述第二肽反应，从而将所述活化的呋喃肽与 所述第二肽。

公开(公告)号：US20130289239A1

公开(公告)日：2013-10-31

申请号：US13996583

申请日：2011-12-23

Applicant: Annemieke Madded ,  Kurt Hoogewijs ,  Annelies Deceuninck ,  Lieselot Carrette

Inventor： Annemieke Madded ,  Kurt Hoogewijs ,  Annelies Deceuninck ,  Lieselot Carrette

IPC: C07K1/107

CPC classification number: C07K1/113 ,  C07K1/006 ,  C07K1/1075 ,  C07K1/1077 ,  C07K1/13 ,  C12N9/0051 ,  C12Y108/01009

Abstract: The present invention relates to a method for cross-linking peptides using an activated furan-moiety. In particular, the present invention provides a method for cross-linking peptides comprising the steps of: a) providing a composition comprising furan-peptides, said furan-peptides comprising at least one amino acid comprising a furan-moiety; b) contacting said composition comprising furan-peptides with second peptides, thereby obtaining a mixture comprising furan-peptides and second peptides; c) adding an activation signal to said mixture of step b), thereby activating said furan-peptides to activated furan-peptides, and d) reacting said activated furan-peptides with said second peptides, thereby cross-linking said activated furan-peptides with said second peptides.

Abstract translation: 本发明涉及使用活化的呋喃部分交联肽的方法。 特别地，本发明提供了交联肽的方法，包括以下步骤：a）提供包含呋喃肽的组合物，所述呋喃肽包含至少一个包含呋喃部分的氨基酸; b）使包含呋喃肽的所述组合物与第二肽接触，从而获得包含呋喃肽和第二肽的混合物; c）向步骤b）的所述混合物中加入活化信号，从而将所述呋喃肽激活成活化的呋喃肽，和d）使所述活化的呋喃肽与所述第二肽反应，从而将所述活化的呋喃肽与 所述第二肽。