公开(公告)号：US20140271599A1

公开(公告)日：2014-09-18

申请号：US14216568

申请日：2014-03-17

Applicant: OrPro Therapeutics, Inc.

Inventor： Peter B. Heifetz

IPC: C12N9/02 ,  A61K38/44

CPC classification number: C12N9/0051 ,  A61K9/0053 ,  A61K9/12 ,  A61K38/44 ,  A61M11/005 ,  C12Y108/01009 ,  C12Y108/0401

Abstract: Disclosed are compositions and methods for decreasing the viscosity and/or cohesiveness of and/or increasing the liquefaction of excessively or abnormally viscous or cohesive mucus or sputum. The composition contains a protein or peptide containing a thioredoxin monocysteinic active site in a reduced state and optionally further contains a reducing system.

Abstract translation: 公开了用于降低过度或异常粘稠或粘性粘液或痰液的粘度和/或内聚力和/或增加液化的组合物和方法。 该组合物包含含有还原状态的硫氧还蛋白一半胱氨酸活性位点的蛋白质或肽，并且任选地还含有还原体系。

公开(公告)号：US08802394B2

公开(公告)日：2014-08-12

申请号：US13127970

申请日：2009-11-12

Applicant: Radu O. Minea ,  Stephen D. Swenson ,  Francis S. Markland, Jr.

Inventor： Radu O. Minea ,  Stephen D. Swenson ,  Francis S. Markland, Jr.

IPC: C12P21/04 ,  C12N1/21

CPC classification number: C12P21/02 ,  C07K14/46 ,  C07K2319/35 ,  C12N9/0036 ,  C12Y108/01007 ,  C12Y108/01009

Abstract: Provided herein are methods for expressing proteins with disulfide bridges such as Vicrostatin (VCN), a chimeric variant of native snake venom disintegrin Contortrostatin (CN). The methods include what is believed to be a more efficient natural selection process that results in generating increased amounts of correctly-folded active conformers of proteins with disulfide bridges. In an aspect, this is achieved by growing Origami B cells in a more optimal redox environment during the induction of heterologous recombinant protein production.

Abstract translation: 本文提供了用二硫键表达蛋白质的方法，例如Vicrostatin（VCN），天然蛇毒解嵌合素抗旋转蛋白（CN）的嵌合变体。 这些方法包括被认为是更有效的天然选择过程，其导致蛋白质与二硫键正确折叠的活性构象异构体的量增加。 在一方面，这是通过在诱导异源重组蛋白质生产期间在更优化的氧化还原环境中生长折纸B细胞来实现的。

公开(公告)号：US20130209432A1

公开(公告)日：2013-08-15

申请号：US13644099

申请日：2012-10-03

Applicant: National Jewish Health

Inventor： Carl W. WHITE

IPC: A61K38/44

CPC classification number: A61K38/44 ,  A61K9/0053 ,  A61K31/385 ,  A61K31/7084 ,  C12N9/0051 ,  C12Y108/01009 ,  C12Y108/0401 ,  Y10S514/824 ,  Y10S514/826 ,  Y10S514/886 ,  A61K2300/00

Abstract: Disclosed are compositions and methods for decreasing the viscosity and/or cohesiveness of and/or increasing the liquefaction of excessively or abnormally viscous or cohesive mucus or sputum. The composition contains a protein or peptide containing a thioredoxin active-site in reduced state and optionally further contains a reducing system.

公开(公告)号：US07074900B2

公开(公告)日：2006-07-11

申请号：US10422422

申请日：2003-04-23

Applicant: Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

Inventor： Bob B. Buchanan ,  Gregorio del Val ,  Rosa M. Lozano ,  Joshua H. Wong ,  Boihan C. Yee ,  Oscar L. Frick

IPC: C07K14/00 ,  C07K14/415 ,  A61K39/35 ,  A61K39/36

CPC classification number: A21D2/26 ,  A21D2/264 ,  A21D2/265 ,  A21D2/266 ,  A21D2/36 ,  A21D8/04 ,  A21D13/064 ,  A23C9/1209 ,  A23C9/1213 ,  A23C9/152 ,  A23J1/12 ,  A23J1/14 ,  A23J3/08 ,  A23J3/18 ,  A23K20/147 ,  A23L5/27 ,  A23L7/109 ,  A23L11/34 ,  A23L13/48 ,  A23L33/17 ,  A61K39/35 ,  A61K2035/122 ,  C07K1/1133 ,  C07K14/415 ,  C07K14/43504 ,  C07K14/46 ,  C07K14/76 ,  C07K14/811 ,  C12N9/0036 ,  C12N9/20 ,  C12P21/02 ,  C12Q1/26 ,  C12Q1/34 ,  C12Y108/01008 ,  C12Y108/01009 ,  Y10S436/904 ,  Y10T436/182

Abstract: Thioredoxin, a small dithiol protein, is a specific reductant for allergenic proteins and particularly allergenic proteins present in pollen and animal and plant sources. All targeted proteins contain disulfide (S—S) bonds that are reduced to the sulfhydryl (SH) level by thioredoxin. The proteins are allergenically active and less digestible in the oxidized (S—S) state. When reduced (SH state), they lose their allergenicity and/or become more digestible. Thioredoxin achieved this reduction when activated (reduced) either by NADPH via NADP-thioredoxin reductase (physiological conditions) or by lipoic acid chemical reductant. Skin tests carried out with sensitized dogs showed that treatment of the pollens with reduced thioredoxin prior to injection eliminated or decreased the allergenicity of the pollen. Studies showed increased digestion of the pollen proteins by pepsin following reduction by thioredoxin. Pollen proteins that have been reduced by thioredoxin are effective and safe immunotherapeutic agents for decreasing or eliminating an animal's allergic reaction that would otherwise occur upon exposure to the non-reduced pollen protein.

公开(公告)号：US20030211511A1

公开(公告)日：2003-11-13

申请号：US10290072

申请日：2002-11-06

Inventor： Steven P. Briggs ,  Bipin K. Dalmia ,  Greg del Val ,  John R. Desjarlais ,  Peter Heifetz ,  Peter Luginbuhl ,  Umesh Muchhal

IPC: C12Q001/68

CPC classification number: C12N9/0036 ,  C07K19/00 ,  C07K2319/00 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y108/01009

Abstract: The present invention relates to the use of a variety of methods for generating functional thioredoxin reductase variants in which at least one physical, chemical or biological property of the variant is altered in a specific and desired manner when compared to the wild-type protein.

公开(公告)号：US20030100743A1

公开(公告)日：2003-05-29

申请号：US10141531

申请日：2002-05-06

Inventor： Bipin K. Dalmia ,  Steven P. Briggs ,  Greg del Val ,  John R. Desjarlais ,  Peter Heifetz ,  Peter Luginbuhl ,  Umesh Muchhal

IPC: C07H021/00 ,  A61K038/00 ,  C12Q001/00

CPC classification number: C12N9/0036 ,  C07K2319/00 ,  C12N15/8242 ,  C12N15/8257 ,  C12Y108/01009 ,  Y02A90/26

Abstract: The present invention relates to the use of a variety of methods for generating functional thioredoxin reductase variants in which at least one physical, chemical or biological property of the variant is altered in a specific and desired manner when compared to the wild-type protein.

Abstract translation: 本发明涉及用于产生功能性硫氧还蛋白还原酶变体的各种方法的用途，其中与野生型蛋白质相比，变体的至少一种物理，化学或生物学特性以特异性和所需方式改变。

公开(公告)号：US20180346918A1

公开(公告)日：2018-12-06

申请号：US16036314

申请日：2018-07-16

Applicant: KOREA ATOMIC ENERGY RESEARCH INSTITUTE

Inventor： Byung Yeoup Chung ,  Sudhir Singh ,  Seung Sik Lee ,  Hyoung Woo Bai ,  Sung Beom Lee

IPC: C12N15/82 ,  C07K14/21 ,  C12N9/08 ,  C12N9/02

CPC classification number: C12N15/8271 ,  C07K14/21 ,  C12N9/0051 ,  C12N9/0065 ,  C12Y108/01 ,  C12Y108/01009 ,  C12Y111/01 ,  C12Y111/01005

Abstract: An AhpF protein has thioredoxin reductase, peroxidase, and chaperone activities and is derived from Pseudomonas aeruginosa, and a use therefor. By using a novel activity of the AhpF of Pseudomonas aeruginosa according to the present invention, it is possible to produce a plant having strong resistance to various environmental stresses such as oxidative stress or heat stress, thereby making it possible to contribute to increasing crop productivity and mass production of useful constituents. In addition, it is possible to prevent desertification and environmental pollution through the development of transformed plants having resistance to high temperatures and drying.

公开(公告)号：US20180291408A1

公开(公告)日：2018-10-11

申请号：US15763799

申请日：2016-09-29

Applicant: ARKEMA FRANCE

Inventor： Georges FREMY ,  Arnaud MASSELIN

IPC: C12P13/12 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04

CPC classification number: C12P13/12 ,  C12N9/0006 ,  C12N9/0051 ,  C12N9/1085 ,  C12Y101/01047 ,  C12Y108/01007 ,  C12Y108/01009 ,  C12Y205/01049

Abstract: Provided is a process for the preparation of L-methionine in an enzymatic reaction utilizing dimethyl disulfide (DMDS) a precursor of L-methionine, and an organic reducing compound. In the process, methyl mercaptan can be formed by the enzymatic hydrogenolysis of the DMDS.

公开(公告)号：US20150044190A1

公开(公告)日：2015-02-12

申请号：US14254565

申请日：2014-04-16

Applicant: National Jewish Health

Inventor： Carl W. White

IPC: A61K38/44 ,  A61K31/7084 ,  C12N9/02

CPC classification number: A61K38/44 ,  A61K9/0053 ,  A61K31/385 ,  A61K31/7084 ,  C12N9/0051 ,  C12Y108/01009 ,  C12Y108/0401 ,  Y10S514/824 ,  Y10S514/826 ,  Y10S514/886 ,  A61K2300/00

Abstract: Disclosed are compositions and methods for decreasing the viscosity and/or cohesiveness of and/or increasing the liquefaction of excessively or abnormally viscous or cohesive mucus or sputum. The composition contains a protein or peptide containing a thioredoxin active-site in reduced state and optionally further contains a reducing system.

公开(公告)号：US20110300579A1

公开(公告)日：2011-12-08

申请号：US13127970

申请日：2009-11-12

Applicant: Radu O. Minea ,  Stephen D. Swenson ,  Francis S. Markland, JR.

Inventor： Radu O. Minea ,  Stephen D. Swenson ,  Francis S. Markland, JR.

IPC: C12P21/02 ,  C12P21/00

CPC classification number: C12P21/02 ,  C07K14/46 ,  C07K2319/35 ,  C12N9/0036 ,  C12Y108/01007 ,  C12Y108/01009

Abstract: Provided herein are methods for expressing proteins with disulfide bridges such as Vicrostatin (VCN), a chimeric variant of native snake venom disintegrin Contortrostatin (CN). The methods include what is believed to be a more efficient natural selection process that results in generating increased amounts of correctly-folded active conformers of proteins with disulfide bridges. In an aspect, this is achieved by growing Origami B cells in a more optimal redox environment during the induction of heterologous recombinant protein production.

Abstract translation: 本文提供了用二硫键表达蛋白质的方法，例如Vicrostatin（VCN），天然蛇毒解嵌合素抗旋转蛋白（CN）的嵌合变体。 这些方法包括被认为是更有效的天然选择过程，其导致蛋白质与二硫键正确折叠的活性构象异构体的量增加。 在一方面，这是通过在诱导异源重组蛋白质生产期间在更优化的氧化还原环境中生长折纸B细胞来实现的。