公开(公告)号：US09139812B2

公开(公告)日：2015-09-22

申请号：US13638262

申请日：2011-02-18

申请人： Kyung Sun Kang ,  Kyung Rok Yu

发明人： Kyung Sun Kang ,  Kyung Rok Yu

IPC分类号： C12N5/071 ,  C12Q1/24 ,  G01N33/50 ,  C12N5/0775 ,  A61K35/12

CPC分类号： C12N5/0602 ,  A61K2035/124 ,  C12N5/0663 ,  C12N5/0665 ,  C12N5/0667 ,  C12Q1/24 ,  G01N33/5073 ,  G01N2333/7055

摘要： The present invention relates to a method for obtaining adult stem cells, which have a surface antigen of CD49f, excellent formation of spheres due to sphere formation and high expression of OCT4 and SOX2, from a cell source including stem cells, and a cell therapeutic agent containing adult stem cells obtained by the method or cells differentiated therefrom as an active ingredient.According to the present invention, adult stem cells derived from spheres are suitable for mass culture of adult stem cells because of more rapid growth thereof compared with stem cells obtained by a known adhesive culture method, have a specific surface antigen so as to be homogeneously obtained by using the specific surface antigen, and are useful for preparing a cell therapeutic agent using the same because of excellent differentiation thereof.

摘要翻译： 本发明涉及一种获得成体干细胞的方法，所述成体干细胞具有CD49f的表面抗原，由球形成的球形成，球形细胞形成，OCT4和SOX2的高表达，来自包括干细胞的细胞源和细胞治疗剂 含有通过其分化的方法获得的成体干细胞作为活性成分。 根据本发明，与通过已知的粘合剂培养方法获得的干细胞相比，由于与成体干细胞的生长更快速的成体干细胞的成体干细胞相比，适于大规模培养，具有比表面抗原以均匀获得 通过使用比表面抗原，由于其优异的分化，可用于制备使用其的细胞治疗剂。

公开(公告)号：US20130089885A1

公开(公告)日：2013-04-11

申请号：US13638262

申请日：2011-02-18

申请人： Kyung Sun Kang ,  Kyung Rok Yu

发明人： Kyung Sun Kang ,  Kyung Rok Yu

IPC分类号： C12N5/071

CPC分类号： C12N5/0602 ,  A61K2035/124 ,  C12N5/0663 ,  C12N5/0665 ,  C12N5/0667 ,  C12Q1/24 ,  G01N33/5073 ,  G01N2333/7055

摘要： The present invention relates to a method for obtaining adult stem cells, which have a surface antigen of CD49f, excellent formation of spheres due to sphere formation and high expression of OCT4 and SOX2, from a cell source including stem cells, and a cell therapeutic agent containing adult stem cells obtained by the method or cells differentiated therefrom as an active ingredient.According to the present invention, adult stem cells derived from spheres are suitable for mass culture of adult stem cells because of more rapid growth thereof compared with stem cells obtained by a known adhesive culture method, have a specific surface antigen so as to be homogeneously obtained by using the specific surface antigen, and are useful for preparing a cell therapeutic agent using the same because of excellent differentiation thereof.

摘要翻译： 本发明涉及一种获得成体干细胞的方法，所述成体干细胞具有CD49f的表面抗原，由球形成的球形成，球形细胞形成，OCT4和SOX2的高表达，来自包括干细胞的细胞源和细胞治疗剂 含有通过其分化的方法获得的成体干细胞作为活性成分。 根据本发明，与通过已知的粘合剂培养方法获得的干细胞相比，由于与成体干细胞的生长更快速的成体干细胞的成体干细胞相比，适于大规模培养，具有比表面抗原以均匀获得 通过使用比表面抗原，由于其优异的分化，可用于制备使用其的细胞治疗剂。