-
公开(公告)号:US08762068B2
公开(公告)日:2014-06-24
申请号:US12607956
申请日:2009-10-28
CPC分类号: G06F19/24 , C12Q1/6813 , C12Q1/6851 , C12Q1/6888 , C12Q2537/143 , C12Q2537/165 , G01N33/53 , G01N33/569 , G01N35/00613 , G01N35/00693 , G06F19/20 , G06K9/6217 , G06K9/626 , C12Q2545/101 , C12Q2545/114
摘要: Methods for determination of threshold values of signatures comprised in an assay are described. Each signature enables detection of a target. The methods determine a probability density function of negative samples and a corresponding false positive rate curve. A false positive criterion is established and a threshold for that signature is determined as a point at which the false positive rate curve intersects the false positive criterion. A method for quantitative analysis and interpretation of assay results together with a method for determination of a desired limit of detection of a signature in an assay are also described.
摘要翻译: 描述了用于确定测定中包含的特征的阈值的方法。 每个签名都可以检测目标。 该方法确定阴性样本的概率密度函数和相应的假阳性率曲线。 建立假阳性标准,并将该签名的阈值确定为假阳性率曲线与假阳性标准相交的点。 还描述了用于定量分析和解释测定结果的方法以及用于测定测定中希望的检测限的检测限的方法。
-
公开(公告)号:US08494780B2
公开(公告)日:2013-07-23
申请号:US12698785
申请日:2010-02-02
IPC分类号: G06F19/24 , G06F19/20 , C12Q1/68 , G01N33/53 , G01N33/569
CPC分类号: G06F19/24 , C12Q1/6813 , C12Q1/6851 , C12Q1/6888 , C12Q2537/143 , C12Q2537/165 , G01N33/53 , G01N33/569 , G01N35/00613 , G01N35/00693 , G06F19/20 , G06K9/6217 , G06K9/626 , C12Q2545/101 , C12Q2545/114
摘要: Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.
摘要翻译: 描述了改善阵列测定性能的方法。 测定荧光强度相关参数与阴性对照值之间的相关性。 然后根据相关性来调整参数。 结果,在不改变其特异性的情况下改进了测定的灵敏度。
-
公开(公告)号:US20110022344A1
公开(公告)日:2011-01-27
申请号:US12698785
申请日:2010-02-02
CPC分类号: G06F19/24 , C12Q1/6813 , C12Q1/6851 , C12Q1/6888 , C12Q2537/143 , C12Q2537/165 , G01N33/53 , G01N33/569 , G01N35/00613 , G01N35/00693 , G06F19/20 , G06K9/6217 , G06K9/626 , C12Q2545/101 , C12Q2545/114
摘要: Methods to improve the performance of an array assay are described. A correlation between fluorescence intensity-related parameters and negative control values of the assay is determined. The parameters are then adjusted as a function of the correlation. As a result, sensitivity of the assay is improved without changes in its specificity.
摘要翻译: 描述了改善阵列测定性能的方法。 测定荧光强度相关参数与阴性对照值之间的相关性。 然后根据相关性来调整参数。 结果,在不改变其特异性的情况下改进了测定的灵敏度。
-
-