公开(公告)号：US07889343B2

公开(公告)日：2011-02-15

申请号：US11631621

申请日：2005-07-01

申请人： Brahim Lounis ,  Laurent Cognet ,  Stephane Berciaud

发明人： Brahim Lounis ,  Laurent Cognet ,  Stephane Berciaud

IPC分类号： G01J4/00 ,  G01N21/41 ,  G30G5/16

CPC分类号： G01N15/1475 ,  G01N21/1717 ,  G01N2021/1725 ,  G01N2021/1731 ,  G01N2021/1757

摘要： Method and system for optical detection of nano-objects in a refracting medium. A nano-object (n_oi) and the refractive medium are illuminated (A) with a periodically amplitude modulated coherent electromagnetic heating wave (HB(Ω)), to generate a specified temperature and refractive index profile in the vicinity of the nano-object, and with a coherent electromagnetic probe wave (PB) to generate an emerging probe wave (EPB(Ω)) having at least one intensity component amplitude modulated by a beat at the modulation frequency of the coherent heating wave. The intensity component amplitude modulated by a beat is detected (C) in the emerging wave (EPB(Ω)), to distinguish and represent this nano-object in the refractive medium. The invention is useful in the detection of nano-objects in an industrial, physiological or intracellular medium.

摘要翻译： 折射介质中纳米物体的光学检测方法和系统。 纳米物体（n_oi）和折射介质用周期性调幅相干电磁加热波（HB（＆OHgr））照射（A），以在纳米物体附近产生规定的温度和折射率分布 并且具有相干的电磁探测波（PB）以产生具有在相干加热波的调制频率处由节拍调制的至少一个强度分量振幅的新兴探测波（EPB（＆OHgr）））。 在出现的波（EPB（＆OHgr））中检测到由拍子调制的强度分量幅度（C），以在折射介质中区分并表示该纳米物体。 本发明可用于检测工业，生理或细胞内介质中的纳米物体。

公开(公告)号：US20070296972A1

公开(公告)日：2007-12-27

申请号：US11631621

申请日：2005-07-01

申请人： Brahim Lounis ,  Laurent Cognet ,  Stephane Berciaud

发明人： Brahim Lounis ,  Laurent Cognet ,  Stephane Berciaud

IPC分类号： G01J4/00 ,  G01N21/41

CPC分类号： G01N15/1475 ,  G01N21/1717 ,  G01N2021/1725 ,  G01N2021/1731 ,  G01N2021/1757

摘要： The invention relates to a method and a system for optical detection of nano-objects in a refracting medium. A nano-object (n_oi) and the refractive medium are illuminated (A) with a periodically amplitude modulated coherent electromagnetic heating wave (HB(Ω)), to generate a specified temperature and refractive index profile in the vicinity of the nano-object, and with a coherent electromagnetic probe wave (PB) to generate an emerging probe wave (EPB(Ω)) having at least one intensity component amplitude modulated by a beat at the modulation frequency of the coherent heating wave. The intensity component amplitude modulated by a beat is detected (C) in the emerging wave (EPB(Ω)), to distinguish and represent this nano-object in the refractive medium. Application to the detection of nano-objects in an industrial, physiological or intracellular medium.

摘要翻译： 本发明涉及一种折射介质中的纳米物体的光学检测方法和系统。 用周期性幅度调制的相干电磁加热波（HB（Omega））照射（A）纳米物体（n_o）和折射介质（A），以产生指定的温度和折射率分布 纳米物体的附近，并具有相干的电磁探测波（PB），以产生具有在相干加热波的调制频率处由节拍调制的至少一个强度分量幅度的新出现的探测波（EPB（Omega） 。 在出现波（EPB（Omega））中检测（C）由拍子调制的强度分量幅度，以区分和表示折射介质中的该纳米物体。 应用于工业，生理或细胞内培养基中纳米物体的检测。

公开(公告)号：US06756591B1

公开(公告)日：2004-06-29

申请号：US10388238

申请日：2003-03-14

申请人： Brahim Lounis ,  Michel Orrit ,  Philippe Tamarat ,  David Boyer ,  Laurent Cognet

发明人： Brahim Lounis ,  Michel Orrit ,  Philippe Tamarat ,  David Boyer ,  Laurent Cognet

IPC分类号： G30G516

CPC分类号： G01N15/1434 ,  G01N21/1717 ,  G01N25/72 ,  G01N2015/0038 ,  G01N2015/144

摘要： A method and device for photothermal imaging tiny metal particles which are immersed in a given medium like a living cell deposited onto a transparent glass slide. The given medium and immersed tiny metal particles are illuminated through separate phase reference laser beam and sensitive probe laser beam, with the sensitive probe laser beam including a heating laser beam undergoing through impingement on the given medium slight phase changes induced by photothermal effect due to a local heating, in the absence of any substantial phase changes to the phase reference laser beam. Illuminating is performed by focusing the separate phase reference and sensitive probe laser beam through the transparent glass slide at a given depth within the given medium and a transmitted phase reference laser beam and a transmitted sensitive probe laser beam undergoing the slight phase changes are generated. An image of the given medium at the given depth is formed through the transmitted phase reference and sensitive probe laser beam and the slight phase changes on the transmitted sensitive probe laser beam with reference to the reference phase laser beam are detected so as to allow each of the tiny metal particles to be imaged as an optical label.

摘要翻译： 一种用于光热成像微小金属颗粒的方法和装置，其浸没在诸如沉积在透明玻璃载片上的活细胞的给定介质中。 给定的介质和浸没的微小金属颗粒通过单独的相位参考激光束和敏感探针激光束照射，灵敏探针激光束包括经受冲击的加热激光束在给定介质上由于光热效应而引起的轻微相变 在相位参考激光束没有任何实质相位变化的情况下进行局部加热。 通过将分离的相位基准和敏感探针激光束聚焦在给定介质内的给定深度处的透明玻璃载玻片上来进行照明，并且产生经历轻微相位变化的透射相位参考激光束和透射敏感探针激光束。 通过透射相位参考和敏感探针激光束形成给定介质在给定深度处的图像，并且检测相对于参考相位激光束在透射的感测探针激光束上的轻微相位变化，以便允许每个 要成像的微小金属颗粒作为光学标签。

公开(公告)号：US20160274107A1

公开(公告)日：2016-09-22

申请号：US15029523

申请日：2014-10-14

申请人： Laurent COGNET ,  Brahim LOUNIS

发明人： Laurent Cognet ,  Brahim Lounis

IPC分类号： G01N33/566 ,  G02B21/16 ,  G01N33/58 ,  G01N21/64

CPC分类号： G01N33/566 ,  G01N21/6428 ,  G01N21/6458 ,  G01N33/582 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/12 ,  G01N2333/71 ,  G02B21/16 ,  G02B21/365

摘要： The present invention is related to a method of studying (i) binding reactions, (ii) dissociation reactions, (iii) competitive binding reactions, or (iv) association reactions between two or more molecules in a biological sample by means of super-resolution imaging, in which method the reaction as such causes an event that forms the basis for the identification and localization-based super-resolution microscopy of the reaction (FIG. 1a).

摘要翻译： 本发明涉及一种研究（i）结合反应，（ii）解离反应，（iii）竞争性结合反应或（iv）通过超分辨率的生物样品中两个或更多个分子之间的缔合反应的方法 在这种方法中，反应如此导致形成反应的鉴定和定位超分辨显微镜的基础的事件（图1a）。