公开(公告)号：US07309595B2

公开(公告)日：2007-12-18

申请号：US10450185

申请日：2001-12-06

申请人： Petrus Jacobus Theodorus Dekker ,  Luppo Edens ,  Robertus Antonius Mijndert Van Der Hoeven ,  Linda De Lange

发明人： Petrus Jacobus Theodorus Dekker ,  Luppo Edens ,  Robertus Antonius Mijndert Van Der Hoeven ,  Linda De Lange

IPC分类号： C12N9/48

CPC分类号： G01N33/573 ,  A23J3/16 ,  A23J3/343 ,  A23J3/344 ,  A23J3/346 ,  A23L2/02 ,  A23L2/84 ,  A23L33/18 ,  A23L33/40 ,  C12H1/003 ,  C12Q1/37 ,  G01N33/56961 ,  G01N2333/38

摘要： The present invention provides An isolated polypeptide which has proline specific endoprotease activity, selected from the group consisting of:(a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 526 of SEQ ID NO:2 or a fragment thereof;(b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO:1 or a fragment thereof which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO:1.

摘要翻译： 本发明提供了具有脯氨酸特异性内切蛋白酶活性的分离多肽，其选自：（a）多肽，其具有与SEQ ID NO：1至526的氨基酸至少具有40％氨基酸序列同一性的氨基酸序列 或其片段;（b）由在低严格条件下与（i）SEQ ID NO：1的核酸序列或其片段至少80％的杂交的多核苷酸编码的多肽， 或超过200个核苷酸的90％以上，优选超过100个核苷酸，更优选至少90％相同，或（ii）与SEQ ID NO：1的核酸序列互补的核酸序列。

公开(公告)号：US20090004693A1

公开(公告)日：2009-01-01

申请号：US11629882

申请日：2005-06-16

申请人： Cornelis Maria Sagt ,  Noel Nicolaas Maria Elisabeth Peij ,  Linda De Lange ,  Martina Beishuizen ,  Serge Petrus Donkers

发明人： Cornelis Maria Sagt ,  Noel Nicolaas Maria Elisabeth Peij ,  Linda De Lange ,  Martina Beishuizen ,  Serge Petrus Donkers

IPC分类号： C12P21/00 ,  C07K14/38 ,  C07H21/04 ,  C12N1/15

CPC分类号： C07K14/395

摘要： The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest.

摘要翻译： 本发明涉及具有对应于SEC61多肽的至少一种活性的活性的多肽，编码这些多肽的多核苷酸及其在制备适合于产生感兴趣多肽的宿主细胞中的用途。 这样的宿主细胞可能具有增加的分泌感兴趣的多肽的能力。

公开(公告)号：US07968312B2

公开(公告)日：2011-06-28

申请号：US11629882

申请日：2005-06-16

申请人： Cornelis Maria Jacobus Sagt ,  Nöel Nicolaas Maria Elisabeth Van Peij ,  Linda De Lange ,  Martina Beishuizen ,  Serge Petrus Donkers

发明人： Cornelis Maria Jacobus Sagt ,  Nöel Nicolaas Maria Elisabeth Van Peij ,  Linda De Lange ,  Martina Beishuizen ,  Serge Petrus Donkers

IPC分类号： C12P21/06 ,  C12N15/00 ,  C12N1/00 ,  C07H21/04

CPC分类号： C07K14/395

摘要： The present invention relates to polypeptides that have an activity corresponding to at least one activity of the SEC61 polypeptide, polynucleotides encoding these polypeptides and the use thereof in the preparation of host cells suitable for production of a polypeptide of interest. Such host cells may have an increased capacity to secrete a polypeptide of interest.

摘要翻译： 本发明涉及具有对应于SEC61多肽的至少一种活性的活性的多肽，编码这些多肽的多核苷酸及其在制备适合于产生感兴趣多肽的宿主细胞中的用途。 这样的宿主细胞可能具有增加的分泌感兴趣的多肽的能力。