-
公开(公告)号：US08003378B2
公开(公告)日：2011-08-23
申请号：US12398825
申请日：2009-03-05
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene LeBoeuf , Chang Y. Chung , David E. Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene LeBoeuf , Chang Y. Chung , David E. Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
-
公开(公告)号：US20120003729A1
公开(公告)日：2012-01-05
申请号：US13175659
申请日：2011-07-01
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
-
公开(公告)号：US07534601B2
公开(公告)日：2009-05-19
申请号：US10525559
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
-
公开(公告)号：US07790443B2
公开(公告)日：2010-09-07
申请号：US10525648
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Mark A. Stremler , Alissa Weaver
发明人： John P. Wikswo , Franz J. Baudenbacher , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Mark A. Stremler , Alissa Weaver
CPC分类号： B01L3/502761 , B01L3/502723 , B01L3/502746 , B01L2200/0668 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0887 , B01L2300/10 , B01L2400/0633 , B01L2400/086 , C12M23/16 , C12M25/14 , C12M29/04 , C12M29/10 , C12M35/08 , C12M41/46
摘要： A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber. The stream of substance includes a substance affecting the growth of cells such as chemokine.
摘要翻译： 具有物质注入能力的生物反应器。 在一个实施例中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面被第二基板的第二表面接收以覆盖空腔，以形成用于容纳电池和液体介质的室。 在第二基板的第二基板的底表面和第一表面之间形成端口。 如此形成的，端口与腔室流体连通，以允许物质流被引入腔室。 物质流被控制以便向腔室提供物质的梯度或浓度梯度。 物质流包括影响细胞生长的物质，如趋化因子。
-
公开(公告)号：US20090215654A1
公开(公告)日：2009-08-27
申请号：US12398825
申请日：2009-03-05
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
-
-
-
-
搜索结果
5 条记录 (耗时 0.016 秒)
