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公开(公告)号：US07790443B2
公开(公告)日：2010-09-07
申请号：US10525648
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Mark A. Stremler , Alissa Weaver
发明人： John P. Wikswo , Franz J. Baudenbacher , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Mark A. Stremler , Alissa Weaver
CPC分类号： B01L3/502761 , B01L3/502723 , B01L3/502746 , B01L2200/0668 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/0887 , B01L2300/10 , B01L2400/0633 , B01L2400/086 , C12M23/16 , C12M25/14 , C12M29/04 , C12M29/10 , C12M35/08 , C12M41/46
摘要： A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a chamber for receiving cells and a liquid medium. A port is formed in the second substrate between the bottom surface and the first surface of the second substrate. As formed, the port is in fluid communication with the chamber to allow a stream of substance to be introduced into the chamber. The stream of substance is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber. The stream of substance includes a substance affecting the growth of cells such as chemokine.
摘要翻译： 具有物质注入能力的生物反应器。 在一个实施例中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面被第二基板的第二表面接收以覆盖空腔，以形成用于容纳电池和液体介质的室。 在第二基板的第二基板的底表面和第一表面之间形成端口。 如此形成的，端口与腔室流体连通，以允许物质流被引入腔室。 物质流被控制以便向腔室提供物质的梯度或浓度梯度。 物质流包括影响细胞生长的物质，如趋化因子。
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公开(公告)号：US20120003729A1
公开(公告)日：2012-01-05
申请号：US13175659
申请日：2011-07-01
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US07534601B2
公开(公告)日：2009-05-19
申请号：US10525559
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Alex Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US20090215654A1
公开(公告)日：2009-08-27
申请号：US12398825
申请日：2009-03-05
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene J. Leboeuf , Chang Y. Chung , David Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US08003378B2
公开(公告)日：2011-08-23
申请号：US12398825
申请日：2009-03-05
申请人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene LeBoeuf , Chang Y. Chung , David E. Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , Ales Prokop , Eugene LeBoeuf , Chang Y. Chung , David E. Cliffel , Frederick R. Haselton , William H. Hofmeister , Charles P. Lin , Lisa J. McCawley , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/00
CPC分类号： B01L3/502761 , B01L3/502746 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/0877 , B01L2300/10 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12M23/16 , C12M29/10
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the cavity so as to form a channel for receiving cells and a liquid medium. In forming the bioreactor, the channel is sized to allow the growth of a layer of cells on a biocompatible coating layer and a flow of liquid in the channel. The flow of liquid is controlled so as to provide a known shear force to the layer of cells. The flow of liquid can be further controlled so as to provide an environment that simulates a vascular space in the channel.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器包括具有第一表面，相对的第二表面和边缘的第一基底。 生物反应器还包括具有第一表面和相对的第二表面的第二基底，其限定具有底表面的空腔，其中底表面位于第一表面和第二表面之间。 第一基板的第一表面由第二基板的第二表面接收以覆盖空腔，以形成用于接收电池和液体介质的通道。 在形成生物反应器时，通道的大小适于允许生物相容性涂层上的细胞层的生长和通道中的液体流。 控制液体的流动以便向细胞层提供已知的剪切力。 可以进一步控制液体的流动，以便提供模拟通道中血管空间的环境。
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公开(公告)号：US07977089B2
公开(公告)日：2011-07-12
申请号：US10525549
申请日：2003-08-27
申请人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器具有第一基底，其具有第一表面和相对的第二表面，在其间限定用于接收细胞和液体介质的腔室。 所述生物反应器还具有将所述室分成第一子室和第二子室的屏障，其中所述阻挡层具有孔隙率以允许所述第一子室和所述第二子室流体连通，并允许至少一个预定类型的泡孔在所述第一室 子房和第二个子房。
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公开(公告)号：US20110294202A1
公开(公告)日：2011-12-01
申请号：US13116174
申请日：2011-05-26
申请人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
发明人： John P. Wikswo , Franz J. Baudenbacher , David Cliffel , Frederick R. Haselton , Eugene J. Leboeuf , Ales Prokop , Randall S. Reiserer , Mark A. Stremler
IPC分类号： C12M1/34
CPC分类号： C12M23/16 , B01L3/5027 , B01L2200/10 , B01L2300/0645 , B01L2300/0681 , B01L2300/0816 , B01L2300/163 , B01L2400/0472 , C12M23/12 , C12M29/10 , C12M41/00 , Y10S435/808
摘要： A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor has a first substrate having a first surface and an opposite second surface, defining a chamber therebetween for receiving the cells and the liquid medium. The bioreactor further has a barrier dividing the chamber into a first subchamber and a second subchamber, wherein the barrier has a porosity to allow the first subchamber and the second subchamber in fluid communication and allow at least one predetermined type of cells to permeate between the first subchamber and the second subchamber.
摘要翻译： 用于在液体培养基中培养活细胞的生物反应器。 在本发明的一个实施方案中，生物反应器具有第一基底，其具有第一表面和相对的第二表面，在其间限定用于接收细胞和液体介质的腔室。 所述生物反应器还具有将所述室分成第一子室和第二子室的屏障，其中所述阻挡层具有孔隙率以允许所述第一子室和所述第二子室流体连通，并允许至少一个预定类型的泡孔在所述第一室 子房和第二个子房。
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8.发明授权公开(公告)号：US07002341B2
公开(公告)日：2006-02-21
申请号：US10650263
申请日：2003-08-27
IPC分类号： G01R33/02
CPC分类号： G01R33/0354
摘要： A method and apparatus performs high resolution imaging. The disclosed apparatus includes a low temperature SQUID sensor mounted in close proximity to a dewar thin window. A radiation shield has an extension surrounding the detection coil.
摘要翻译： 一种方法和装置执行高分辨率成像。 所公开的设备包括安装在靠近杜瓦瓶薄窗的低温SQUID传感器。 辐射屏蔽具有围绕检测线圈的延伸。
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公开(公告)号：US20090068700A1
公开(公告)日：2009-03-12
申请号：US12229503
申请日：2008-08-22
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： A device and methods for monitoring status of at least one cell, wherein the cell has a membrane forming a substantially enclosed structure and defining an intracellular space therein. In one embodiment of the present invention, the device includes a first substrate having a first surface and an opposite second surface, a second substrate supported by the first substrate, the second substrate having a first surface, an opposite second surface, a body portion between the first surface and the second surface, a first side surface and an opposite second side surface, wherein the body portion defines a first passage between the first side surface and the second side surface and an opening on the first surface of the second substrate and in fluid communication with the first passage, and sidewalls positioned above the first surface of the second substrate. In one operation mode, when a first medium is introduced into the first passage, the intracellular space of the cell is in fluid communication with the first passage with the first medium, a sensor measures the response of the cell to the first medium.
摘要翻译： 一种用于监测至少一个细胞状态的装置和方法，其中所述细胞具有形成基本封闭结构并在其中限定细胞内空间的膜。 在本发明的一个实施例中，该装置包括具有第一表面和相对的第二表面的第一基底，由第一基底支撑的第二基底，第二基底具有第一表面，相对的第二表面， 第一表面和第二表面，第一侧表面和相对的第二侧表面，其中主体部分限定在第一侧表面和第二侧表面之间的第一通道和在第二基板的第一表面上的开口， 与第一通道流体连通，以及位于第二基板的第一表面上方的侧壁。 在一种操作模式中，当第一介质被引入第一通道时，电池的细胞内空间与第一介质与第一通道流体连通，传感器测量电池对第一介质的响应。
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公开(公告)号：US07981649B2
公开(公告)日：2011-07-19
申请号：US12229503
申请日：2008-08-22
CPC分类号： G01N33/5005 , C12M41/32 , G01N33/5023 , G01N33/5038 , G01N33/5041 , G01N2015/1006 , Y10T436/115831
摘要： A device and methods for monitoring status of at least one cell, wherein the cell has a membrane forming a substantially enclosed structure and defining an intracellular space therein. In one embodiment of the present invention, the device includes a first substrate having a first surface and an opposite second surface, a second substrate supported by the first substrate, the second substrate having a first surface, an opposite second surface, a body portion between the first surface and the second surface, a first side surface and an opposite second side surface, wherein the body portion defines a first passage between the first side surface and the second side surface and an opening on the first surface of the second substrate and in fluid communication with the first passage, and sidewalls positioned above the first surface of the second substrate. In one operation mode, when a first medium is introduced into the first passage, the intracellular space of the cell is in fluid communication with the first passage with the first medium, a sensor measures the response of the cell to the first medium.
摘要翻译： 一种用于监测至少一个细胞状态的装置和方法，其中所述细胞具有形成基本封闭结构并在其中限定细胞内空间的膜。 在本发明的一个实施例中，该装置包括具有第一表面和相对的第二表面的第一基底，由第一基底支撑的第二基底，第二基底具有第一表面，相对的第二表面， 第一表面和第二表面，第一侧表面和相对的第二侧表面，其中主体部分限定在第一侧表面和第二侧表面之间的第一通道和在第二基板的第一表面上的开口， 与第一通道流体连通，以及位于第二基板的第一表面上方的侧壁。 在一种操作模式中，当第一介质被引入第一通道时，电池的细胞内空间与第一介质与第一通道流体连通，传感器测量电池对第一介质的响应。
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